Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (HRP)

Details for Product No. ABIN250721, Supplier: Log in to see
Antigen
  • MGC69006
  • MGC89867
  • MAP1LC3A
  • map1lc3a
  • Anx3
  • LC3
  • LRRGT00047
  • ATG8E
  • LC3A
  • MAP1ALC3
  • MAP1BLC3
  • 1010001H21Rik
  • 4922501H04Rik
  • LC3a
  • zgc:77094
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • Lc3
  • b3gnt5
  • fb12f12
  • wu:fb12f12
  • wu:fe50g08
  • microtubule-associated protein 1 light chain 3 alpha
  • annexin A3
  • microtubule-associated protein 1 light chain 3 beta
  • UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5a
  • map1lc3a
  • MAP1LC3A
  • Anxa3
  • Map1lc3a
  • map1lc3b
  • b3gnt5a
Alternatives
anti-Human Microtubule-Associated Protein 1 Light Chain 3 alpha antibody for Immunofluorescence (fixed cells)
Epitope
AA 25-121, Internal Region
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Reactivity
Dog (Canine), Fish, Human, Mouse (Murine), Plant, Rat (Rattus), Zebrafish
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
HRP
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Application
ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
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Immunogen A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [UniProt# Q9GZQ8].
Specificity This antibody detects both LC3A and LC3B.
Purification Immunogen affinity purified
Alternative Name LC3/MAP1LC3A (MAP1LC3A Antibody Abstract)
Background Gene Symbol: MAP1LC3A
Gene ID 84557
UniProt Q9GZQ8
Pathways Autophagy
Application Notes Western Blot, ELISA, Immunohistochemistry-Paraffin, Immunohistochemistry-FrozenThis LC3 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence (PMID 21545732), Immunoprecipitation and Immunohistochemistry paraffin embedded sections. By Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. In ICC/IF, cytoplasmic staining was observed in HeLa cells.Use in FLOW cytometry reported in scientific literature ( PMID 24419333)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer PBS
Buffer contains: No Preservative
Preservative Without preservative
Storage 4 °C
Storage Comment Store at 4°C in the dark.
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (HRP) (ABIN250721) anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (HRP)
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (HRP) (ABIN250721) Staining of human brain, cerebral cortex, cell crocesses in gray matter using ABIN250...
Product cited in: Lee, Cao, Mostoslavsky, Lombard, Liu, Bruns, Tsokos, Alt, Finkel: "A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3374-9, 2008 (PubMed).

Swerdlow, McColl, Rong, Lam, Gupta, Distelhorst: "Apoptosis inhibition by Bcl-2 gives way to autophagy in glucocorticoid-treated lymphocytes." in: Autophagy, Vol. 4, Issue 5, pp. 612-20, 2008 (PubMed).

Zhang, Yu, Pan, Hu, Hao, Cai, Zhu, Yu, Xie, Ma, Yuan: "Small molecule regulators of autophagy identified by an image-based high-throughput screen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 48, pp. 19023-8, 2007 (PubMed).

Kabeya, Mizushima, Ueno, Yamamoto, Kirisako, Noda, Kominami, Ohsumi, Yoshimori: "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing." in: The EMBO journal, Vol. 19, Issue 21, pp. 5720-8, 2000 (PubMed).

Background publications Mizushima, Yamamoto, Hatano, Kobayashi, Kabeya, Suzuki, Tokuhisa, Ohsumi, Yoshimori: "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 657-68, 2001 (PubMed).