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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (HRP)

Details for Product No. ABIN250721, Supplier: Log in to see
Antigen
  • 1010001H21Rik
  • 4922501H04Rik
  • Anx3
  • ATG8E
  • b3gnt5
  • fb12f12
  • Lc3
  • LC3
  • LC3a
  • LC3A
  • LRRGT00047
  • MAP1ALC3
  • MAP1BLC3
  • Map1lc3
  • map1lc3a
  • MAP1LC3A
  • MGC69006
  • MGC89867
  • wu:fb12f12
  • wu:fb60g11
  • wu:fe50g08
  • zgc:56434
  • zgc:77094
Epitope
AA 25-121, Internal Region
43
38
28
26
24
23
14
14
14
14
13
13
12
12
11
10
8
8
7
7
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Fish, Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
318
110
104
32
30
16
14
10
9
6
6
5
3
1
1
Host
Rabbit
279
33
5
4
Clonality
Polyclonal
Conjugate
HRP
23
21
19
18
14
14
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
259
145
138
81
65
33
27
24
20
12
10
10
6
1
Supplier
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Immunogen A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [UniProt# Q9GZQ8].
Specificity This antibody detects both LC3A and LC3B.
Cross-Reactivity (Details) Predicted to react with Xenopus,bovine based on 100% sequence homology.
Purification Immunogen affinity purified
Alternative Name LC3/MAP1LC3A (MAP1LC3A Antibody Abstract)
Background Gene Symbol: MAP1LC3A
UniProt Q9GZQ8
Application Notes Western Blot 2 μg/mL, Immunohistochemistry 1:200-1:400, Immunoprecipitation 20 μg/ 500 μg of lysate, Immunohistochemistry-Paraffin 1:200-1:400
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer PBS
Buffer contains: No Preservative
Preservative Without preservative
Storage 4 °C
Storage Comment Store at 4°C in the dark.
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (HRP) (ABIN250721) anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (HRP)
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 25-121), (Internal Region) antibody (HRP) (ABIN250721) Staining of human brain, cerebral cortex, cell crocesses in gray matter using ABIN250...
Product cited in: Lee, Cao, Mostoslavsky, Lombard, Liu, Bruns, Tsokos, Alt, Finkel: "A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3374-9, 2008 (PubMed).

Swerdlow, McColl, Rong, Lam, Gupta, Distelhorst: "Apoptosis inhibition by Bcl-2 gives way to autophagy in glucocorticoid-treated lymphocytes." in: Autophagy, Vol. 4, Issue 5, pp. 612-20, 2008 (PubMed).

Zhang, Yu, Pan, Hu, Hao, Cai, Zhu, Yu, Xie, Ma, Yuan: "Small molecule regulators of autophagy identified by an image-based high-throughput screen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 48, pp. 19023-8, 2007 (PubMed).

Kabeya, Mizushima, Ueno, Yamamoto, Kirisako, Noda, Kominami, Ohsumi, Yoshimori: "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing." in: The EMBO journal, Vol. 19, Issue 21, pp. 5720-8, 2000 (PubMed).

Background publications Mizushima, Yamamoto, Hatano, Kobayashi, Kabeya, Suzuki, Tokuhisa, Ohsumi, Yoshimori: "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 657-68, 2001 (PubMed).