anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (C-Term)

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C-Term, AA 1300-1367
(77), (53), (52), (38), (24), (19), (18), (18), (16), (14), (14), (14), (12), (12), (12), (12), (10), (9), (8), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(553), (295), (165), (6), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
(514), (99), (39), (3), (2), (2), (1)
This VEGFR2 antibody is un-conjugated
(39), (22), (19), (16), (12), (11), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
(442), (255), (227), (124), (113), (110), (40), (29), (24), (20), (16), (16), (8), (6), (6), (2), (1), (1)
Pubmed 4 references available
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Quantity 0.1 mL
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Immunogen A synthetic peptide made to a C-terminal region of the mouse VEGF Receptor 2 protein (between residues 1300-1367). Immunogen sequence has 79% homology to rat. Hamster reactivity reported in scientific literature (PMID: 23763710)Marker Endothelial Cell Marke
Specificity This has only been shown to recognize the native form (180-190 kDa protein) of VEGFR-2, known as Flk-1 / KDR / VEGFR 2 (Vascular Endothelial Growth Factor Receptor 2), though it was raised against the precursor form.
Cross-Reactivity (Details) Predicted to react with mouse based on 100% sequence homology.
Purification affinity purified
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background Receptors for VEGF, VEGFB and PGF have a tyrosine-protein kinase activity. TheVEGF-kinase ligand/receptor signaling system plays a key role in vascular developmentand regulation of vascular permeability. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, arethought to be important for the development of embryonic vasculature. It has beenshown that an alternately spliced form of FLT1 produces a soluble protein, termedsFLT1, which binds vascular endothelial growth factor with high affinity, playing aninhibitory role in angiogenesis. Alternate Names: anti-VEGFR-2 antibody, anti-VEGFR 2 antibody, anti-Protein-tyrosine kinase receptorflk-1 antibody, anti-Fetal liver kinase 1 antibody, anti-Kinase NYK antibody, anti-FLK1antibody, anti-KDR antibody, anti-Kinase insert domain receptor antibody, anti-KRD1antibody, anti-Ly73 antibody, anti-Protein tyrosine kinase receptor FLK1 antibody,anti-Vascular endothelial growth factor receptor 2 antibody.
Gene Symbol: KDR
Gene ID 3791
UniProt P35918
Research Area Cytokines, Receptors
Pathways RTK Signaling
Application Notes This VEGF Receptor 2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen at ~150 kDa with CSF-1R/VEGFR2 chimera transfected lysates representing VEGFR-2. A non-specific band is also seen at ~85 kDa. In ICC/IF punctate membrane staining was observed in Hela cells.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10, Western Blot 1 µg/mL
Protocol Western blot protocol specific for VEGF Receptor 2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS overnight at 4 °C.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (C-Term) (ABIN250774) Detection of VEGFR2 doublet in CSF-IR/VEGFR2 chimera transfected lysate using ABIN250...
Product cited in: Hu-Lowe, Zou, Grazzini et al.: "Nonclinical antiangiogenesis and antitumor activities of axitinib (AG-013736), an oral, potent, and selective inhibitor of vascular endothelial growth factor receptor tyrosine kinases 1, 2, 3." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 22, pp. 7272-83, 2008 (PubMed).

Takahashi, Hattori, Iwamatsu et al.: "A novel snake venom vascular endothelial growth factor (VEGF) predominantly induces vascular permeability through preferential signaling via VEGF receptor-1." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46304-14, 2004 (PubMed).

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000 (PubMed).

Background publications Gluzman-Poltorak, Cohen, Shibuya et al.: "Vascular endothelial growth factor receptor-1 and neuropilin-2 form complexes." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18688-94, 2001 (PubMed).