VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 1300-1367)

Details for Product anti-KDR Antibody No. ABIN250774, Supplier: Log in to see
Antigen
  • vegfr-2
  • KDR
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • Vegfr-2
  • CD309
  • FLK1
  • VEGFR
  • FLK-1
  • flk-1
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • fk52c05
  • flk
  • kdr
  • kdra
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fk52c05
  • vascular endothelial growth factor receptor 2
  • kinase insert domain protein receptor
  • kinase insert domain receptor
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • kinase insert domain receptor like
  • vascular endothelial growth factor receptor-2
  • vegfr-2
  • Kdr
  • KDR
  • kdr
  • kdrl
Alternatives
anti-Human VEGFR2 antibody for Blocking Antibody
Epitope
AA 1300-1367, C-Term, Native Form
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Reactivity
Hamster, Human
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
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Options
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Immunogen A synthetic peptide made to a C-terminal region of the mouse VEGF Receptor 2 protein (between residues 1300-1367). [Swiss-Prot# P35918]
Specificity This has only been shown to recognize the native form (180-190 kDa protein) of VEGFR-2, known as Flk-1/ KDR/ VEGFR 2 (Vascular Endothelial Growth Factor Receptor 2), though it was raised against the precursor form.
Purification Immunogen affinity purified
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background Gene Symbol: KDR
Molecular Weight Theoretical MW: 150 kDa
Gene ID 3791
UniProt P35918
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity
Application Notes Western Blot 1 μg/mL, Immunocytochemistry/Immunofluorescence 1:10This VEGF Receptor 2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen at ~150 kDa with CSF-1R/VEGFR2 chimera transfected lysates representing VEGFR-2. A non-specific band is also seen at ~85 kDa. In ICC/IF punctate membrane staining was observed in Hela cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot protocol specific for VEGF Receptor 2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS overnight at 4 °C.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 1300-1367) (ABIN250774) Detection of VEGFR2 doublet in CSF-IR/VEGFR2 chimera transfected lysate using ABIN250...
Immunofluorescence (IF) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (AA 1300-1367) (ABIN250774) Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody [ABIN250774] - VEG...
Product cited in: Zhou, Rogers, Lee, Sun, Yao, Mao, Kong: "Oral Mucosa Harbors a High Frequency of Endothelial Cells: A Novel Postnatal Cell Source for Angiogenic Regeneration." in: Stem cells and development, Vol. 26, Issue 2, pp. 91-101, 2016 (PubMed). Method employed by authors: Flow Cytometry (FACS) (Sample species: Rat (Rattus)).

Lim, Yap, Lim, Goh, Ng: "Identification of autocrine growth factors secreted by CHO cells for applications in single-cell cloning media." in: Journal of proteome research, Vol. 12, Issue 7, pp. 3496-510, 2013 (PubMed). (Sample species: Hamster). Further details: Western Blotting

Roan, Fang, Chang, Hsu, Huang, Chiou, Tsai, Lam: "Rosuvastatin improves vascular function of arteriovenous fistula in a diabetic rat model." in: Journal of vascular surgery, Vol. 56, Issue 5, pp. 1381-9.e1, 2012 (PubMed). (Sample species: Rat (Rattus)). Further details: Flow Cytometry

Hu-Lowe, Zou, Grazzini, Hallin, Wickman, Amundson, Chen, Rewolinski, Yamazaki, Wu, McTigue, Murray, Kania, OConnor, Shalinsky, Bender: "Nonclinical antiangiogenesis and antitumor activities of axitinib (AG-013736), an oral, potent, and selective inhibitor of vascular endothelial growth factor receptor tyrosine kinases 1, 2, 3." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 22, pp. 7272-83, 2008 (PubMed).

Takahashi, Hattori, Iwamatsu, Takizawa, Shibuya: "A novel snake venom vascular endothelial growth factor (VEGF) predominantly induces vascular permeability through preferential signaling via VEGF receptor-1." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46304-14, 2004 (PubMed).

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000 (PubMed).

Background publications Gluzman-Poltorak, Cohen, Shibuya, Neufeld: "Vascular endothelial growth factor receptor-1 and neuropilin-2 form complexes." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18688-94, 2001 (PubMed).