Zinc Metalloproteinase, Ste24 (Zmpste24) (AA 1-100), (N-Term) antibody

Details for Product No. ABIN250782, Supplier: Log in to see
Antigen
  • DDBDRAFT_0189115
  • DDBDRAFT_0237846
  • DDB_0189115
  • DDB_0237846
  • F2N1.21
  • F2N1_21
  • STE24
  • FACE-1
  • FACE1
  • HGPS
  • PRO1
  • Ste24p
  • A530043O15Rik
  • D030046F19
  • Face-1
  • MADB
  • fi45a02
  • wu:fi45a02
  • zgc:55655
  • zinc metallopeptidase (STE24 homolog, S. cerevisiae)
  • zinc metallopeptidase STE24
  • zinc metallopeptidase STE24 homolog (S. cerevisiae)
  • CAAX prenyl protease
  • peptidase family M48 family protein
  • zinc metallopeptidase, STE24
  • zinc metallopeptidase, STE24 homolog
  • ZMPSTE24
  • zmpste24
  • ATSTE24
  • Zmpste24
Epitope
N-Term, AA 1-100
57
19
15
13
13
12
8
4
3
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Zebrafish
123
34
12
12
2
2
2
2
1
1
1
1
Host
Rabbit
109
16
Clonality
Polyclonal
Conjugate
Un-conjugated
11
11
9
7
7
7
5
3
3
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
120
59
27
12
12
9
2
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen A synthetic peptide made to an N-terminal portion of the human ZMPSTE24 protein sequence (between residues 1-100). [Swiss-Prot O75844]
Purification Immunogen affinity purified
Alternative Name ZMPSTE24 (Zmpste24 Antibody Abstract)
Background Gene Symbol: ZMPSTE24
Molecular Weight Theoretical MW: 54 kDa
Gene ID 10269
UniProt O75844
Research Area Proteolysis / Ubiquitin, Cytoskeleton
Application Notes Western Blot 1.0-2.0 μg/mL, Immunocytochemistry/ImmunofluorescenceThis ZMPSTE24 antibody is useful for Western Blot, where a band is seen at ~54 kDa representing ZMPSTE24 protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for ZMPSTE24 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS overnight at 4 degrees Celcius.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-ZMPSTE24 primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Zinc Metalloproteinase, Ste24 (Zmpste24) (AA 1-100), (N-Term) antibody (ABIN250782) Detection of ZMPSTE24 in human testis using ABIN250782.
Immunofluorescence (IF) image for anti-Zinc Metalloproteinase, Ste24 (Zmpste24) (AA 1-100), (N-Term) antibody (ABIN250782) Immunocytochemistry/Immunofluorescence: ZMPSTE24 Antibody [ABIN250782] - analysis of ...
Product cited in: Ponnusamy, Zhang, Kadam, Lin, Lim, Sandhu, Kothandaraman, Mahyuddin, Biswas, Venkat, Hew, Joshi, Chung, Choolani: "Membrane proteins of human fetal primitive nucleated red blood cells." in: Journal of proteomics, Vol. 75, Issue 18, pp. 5762-73, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Coffinier, Hudon, Farber, Chang, Hrycyna, Young, Fong: "HIV protease inhibitors block the zinc metalloproteinase ZMPSTE24 and lead to an accumulation of prelamin A in cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 33, pp. 13432-7, 2007 (PubMed).

Young, Fong, Michaelis: "Prelamin A, Zmpste24, misshapen cell nuclei, and progeria--new evidence suggesting that protein farnesylation could be important for disease pathogenesis." in: Journal of lipid research, Vol. 46, Issue 12, pp. 2531-58, 2005 (PubMed).

Background publications Corrigan, Kuszczak, Rusinol, Thewke, Hrycyna, Michaelis, Sinensky: "Prelamin A endoproteolytic processing in vitro by recombinant Zmpste24." in: The Biochemical journal, Vol. 387, Issue Pt 1, pp. 129-38, 2005 (PubMed).