Tripartite Motif Containing 63 (TRIM63) (Internal Region) antibody

Details for Product No. ABIN250793
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Internal Region
(8), (6), (4), (3), (2), (2), (1), (1), (1), (1)
(60), (32), (29), (19), (12), (9)
(41), (15), (6), (1)
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Immunohistochemistry (IHC), ELISA
(31), (20), (17), (15), (9), (4), (3), (2), (2), (2), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN250793
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Immunogen Synthetic peptide: C-QQYQEQLDKSTKLVE, representing the internal region of the human protein according to NP_115977.
Isotype IgG
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Human, Mouse, Rat, Dog, Cow
Purification affinity purified
Alternative Name TRIM63 / MURF1 / RNF28
Background Gene ID(s): 84676 (human). Alternate Names: anti-TRIM63 antibody, anti-tripartite motif-containing 63antibody, anti-HGNC:16007antibody, anti-FLJ32380 antibody, anti-IRF antibody, anti-MURF1 antibody, anti-MURF2antibody, anti-RNF28 antibody, anti-SMRZantibody, anti-iris ring finger protein antibody,anti-muscle specific ring finger protein 1 antibody, anti-muscle specific ring finger protein2 antibody, anti-ring finger protein 28 antibody, anti-striated muscle RING zinc fingerprotein antibody.
Gene Symbol: TRIM63
Gene ID 84676
NCBI Accession NP_115977
Application Notes Preliminary experiments gave an approx 75kDa band in human heart and skeletal muscle lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Recommended dilutions: Immunohistochemistry 1:10-1:500, Peptide ELISA Detection Limit 1:32000
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris-saline (pH 7.3) with 0.5 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
General Willis, Rojas, Li et al.: "Muscle ring finger 1 mediates cardiac atrophy in vivo." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 4, pp. H997-H1006, 2009 (PubMed).

Cao, Kim, Lecker: "Ubiquitin-protein ligases in muscle wasting." in: The international journal of biochemistry & cell biology, Vol. 37, Issue 10, pp. 2088-97, 2005 (PubMed).

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