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Crystallin, alpha B (CRYAB) (AA 100-175), (C-Term) antibody

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Synonyms CRYAB, CMD1II, CRYA2, CTPP2, CTRCT16, HSPB5, MFM2, Crya-2, Crya2, HspB5, AACRYA
C-Term, AA 100-175
(35), (33), (28), (21), (19), (13), (12), (11), (9), (7), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Cow (Bovine), Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(375), (173), (149), (70), (21), (20), (2), (2), (2), (1), (1), (1)
(205), (159), (16), (1)
(16), (15), (13), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Western Blotting (WB)
(290), (175), (126), (69), (66), (42), (31), (17), (17), (7), (4), (2), (1)
Pubmed 4 references available
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Quantity 25 μL
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Immunogen A synthetic peptide made to a C-terminal region of the human Crystallin AB protein (within residues 100-175). Immunogen sequence has 92% homology to mouse.
Cross-Reactivity (Details) Predicted to react with rat, sheep, rabbit, monkey, hamster, cow,pig based on 100% sequence homology.
Purification affinity purified
Alternative Name Alpha-crystallin B chain (CRYAB Antibody Abstract)
Background Crystallin AB (CryAB) is a developmentally regulated small heat shock protein known toprotect cells from stress damage. It may also play an important role in the Golgireorganization during cell division. CryAB is known to be associated with a number ofneurodegenerative pathologies such as Alzheimer disease amd multiple sclerosis. Aswell, it has been found to be expressed in breast tumors of patients with shorter survival,suggesting that it may be a useful marker in identifying aggressive breast cancer. Alternate Names: anti-Alpha crystallin B chain antibody, anti-CryAB antibody, anti-Alpha (B)-crystallinantibody, anti-Rosenthal fiber componet antibody, anti-Heat-shock protein beta-5antibody, anti-HspB5 antibody, anti-CryA2 antibody, anti-AACRYA antibody, anti-Alphacrystallin B chain antibody, anti-CRYA2 antibody, anti-Crystallin alpha polypeptide 2antibody, anti-CTPP 2 antibody, anti-CTPP2 antibody, anti-Heat shock 20 kD likeprotein antibody, anti-Heat shock protein beta 5 antibody, anti-HSPB5 antibody,anti-Rosenthal fiber component antibody.
Gene Symbol: CRYAB
Gene ID 1410, 15507
UniProt P02511
Research Area Neurology, Enzymes
Application Notes This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. **CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.
Recommended dilutions: Western Blot 0.5-1 µg/mL
Protocol Protocol specific for Crystallin AB Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %, Bis-Tris) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS, overnight at 4 °C.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-crystallin AB primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Pierce's ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.9 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Crystallin, alpha B (CRYAB) (AA 100-175), (C-Term) antibody (ABIN250949) anti-Crystallin, alpha B (CRYAB) (AA 100-175), (C-Term) antibody
Product cited in: Wu, Glickstein, Liu et al.: "The anaphase-promoting complex coordinates initiation of lens differentiation." in: Molecular biology of the cell, Vol. 18, Issue 3, pp. 1018-29, 2007 (PubMed).

Sanbe, Osinska, Villa et al.: "Reversal of amyloid-induced heart disease in desmin-related cardiomyopathy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 38, pp. 13592-7, 2005 (PubMed).

Liu, Du, Wang et al.: "Crystallin {gamma}B-I4F mutant protein binds to {alpha}-crystallin and affects lens transparency." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 25071-8, 2005 (PubMed).

Gangalum, Schibler, Bhat: "Small heat shock protein alphaB-crystallin is part of cell cycle-dependent Golgi reorganization." in: The Journal of biological chemistry, Vol. 279, Issue 42, pp. 43374-7, 2004 (PubMed).

Catalog No. ABIN250949
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