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CRYAB antibody (Crystallin, alpha B) (C-Term)

Details for Product anti-CRYAB Antibody No. ABIN250949, Supplier: Log in to see
Antigen
  • CRYAB
  • CMD1II
  • CRYA2
  • CTPP2
  • CTRCT16
  • HSPB5
  • MFM2
  • Crya-2
  • Crya2
  • HspB5
  • AACRYA
  • crystallin, alpha B
  • hypothetical protein
  • CRYAB
  • alpha-B-crystallin
  • Cryab
Epitope
C-Term, AA 100-175
46
35
33
26
24
22
20
13
12
12
11
9
7
6
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
444
197
192
91
29
23
3
2
2
1
1
1
Host
Rabbit
251
182
17
1
Clonality
Polyclonal
Conjugate
This CRYAB antibody is un-conjugated
20
19
18
9
9
9
9
9
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
5
4
4
4
3
3
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
354
195
144
76
76
66
58
25
19
7
5
2
1
Options
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Immunogen A synthetic peptide made to a C-terminal region of the human Crystallin AB protein (within residues 100-175). [Swiss-Prot P02511]
Cross-Reactivity (Details) Predicted to react with rat, sheep, rabbit, monkey, hamster, cow,pig based on 100% sequence homology.
Purification Immunogen affinity purified
Alternative Name AlphaB Crystallin/CRYAB (CRYAB Antibody Abstract)
Background Gene Symbol: CRYAB
Gene ID 1410
UniProt P02511
Application Notes Western Blot 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1: 50 - 1:150This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. **CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Crystallin AB Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %, Bis-Tris) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS, overnight at 4 °C.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-crystallin AB primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Pierce's ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.9 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-CRYAB antibody (Crystallin, alpha B) (C-Term) (ABIN250949) anti-Crystallin, alpha B (CRYAB) (AA 100-175), (C-Term) antibody
Western Blotting (WB) image for anti-CRYAB antibody (Crystallin, alpha B) (C-Term) (ABIN250949) Western Blot: AlphaB Crystallin/CRYAB Antibody [ABIN250947] - Analysis of Lane 1: hum...
Immunofluorescence (IF) image for anti-CRYAB antibody (Crystallin, alpha B) (C-Term) (ABIN250949) Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [ABIN250947]...
Product cited in: Chauhan, Nelson, Marriott, Bost: "Alpha beta-crystallin expression and presentation following infection with murine gammaherpesvirus 68." in: Autoimmunity, Vol. 46, Issue 6, pp. 399-408, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: ELISA,Flow Cytometry

Wu, Glickstein, Liu, Fujita, Li, Yang, Duvoisin, Wan: "The anaphase-promoting complex coordinates initiation of lens differentiation." in: Molecular biology of the cell, Vol. 18, Issue 3, pp. 1018-29, 2007 (PubMed).

Liu, Du, Wang, Huang, Ding, McDonald, Yates, Beutler, Horwitz, Gong: "Crystallin {gamma}B-I4F mutant protein binds to {alpha}-crystallin and affects lens transparency." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 25071-8, 2005 (PubMed).

Sanbe, Osinska, Villa, Gulick, Klevitsky, Glabe, Kayed, Robbins: "Reversal of amyloid-induced heart disease in desmin-related cardiomyopathy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 38, pp. 13592-7, 2005 (PubMed).

Gangalum, Schibler, Bhat: "Small heat shock protein alphaB-crystallin is part of cell cycle-dependent Golgi reorganization." in: The Journal of biological chemistry, Vol. 279, Issue 42, pp. 43374-7, 2004 (PubMed).