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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) antibody
|Synonyms||cb633, dnmt3b, MGC152725, DNMT3B, LOC100218113, ICF, M.HsaIIIB|
Alternatives Immunoprecipitation (IP), Western Blotting (WB)
|7 references available|
|Quantity||25 µl (Variants)|
|Price||Product not available in this region.|
|Immunogen||This antibody was raised against residues 4-101 of the human DNMT3b protein.|
|Cross-Reactivity||Human, Mouse (Murine)|
|Description||Inactivation of tumor suppressor genes is a key component of the development of allcommon forms of human cancers. Aberrant methylation may contribute to many of thechanges, both physiological and pathological, associated with aging, including tumordevelopment. Hypermethylation of CpG island-containing promoters is associated withthe inactivation of genes important to tumor angiogenesis and progression. DNAmethytransferase (DNMT3b) is necessary for the maintenance of the methylationprocess. Alternate Names: anti-ICF antibody, anti-M.HsaIIIB antibody.|
|Specificity||This antibody is specific for DNMT3b. Species Reactivity: ABIN151734 reacts with human and mouse proteins. Other species have not been tested.|
|Application Notes||Suggested working dilutions: immunoprecipitation 1:1,000,Western Blot 1:1,000. Positive Controls: HCT116 cell lysates.|
|Buffer||Preservative: No preservative.|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Restrictions||For Research Use only|
Rhee, Bachman, Park et al.: "DNMT1 and DNMT3b cooperate to silence genes in human cancer cells." in: Nature, Vol. 416, Issue 6880, pp. 552-6, 2002 (PubMed).
Beaulieu, Morin, Chute et al.: "An essential role for DNA methyltransferase DNMT3B in cancer cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28176-81, 2002 (PubMed).
Weisenberger, Velicescu, Preciado-Lopez et al.: "Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells." in: Gene, Vol. 298, Issue 1, pp. 91-9, 2002 (PubMed).
Robert, Morin, Beaulieu et al.: "DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells." in: Nature genetics, Vol. 33, Issue 1, pp. 61-5, 2003 (PubMed).
Xiong, Dowdy, Podratz et al.: "Histone deacetylase inhibitors decrease DNA methyltransferase-3B messenger RNA stability and down-regulate de novo DNA methyltransferase activity in human endometrial cells." in: Cancer research, Vol. 65, Issue 7, pp. 2684-9, 2005 (PubMed).
Morey, Smiraglia, James et al.: "DNA methylation pathway alterations in an autochthonous murine model of prostate cancer." in: Cancer research, Vol. 66, Issue 24, pp. 11659-67, 2006 (PubMed).
Morey Kinney, Smiraglia, James et al.: "Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model." in: Molecular cancer research : MCR, Vol. 6, Issue 8, pp. 1365-74, 2008 (PubMed).
|Hosts||Rabbit (17), Mouse (7), Chicken (2)|
|Reactivities||Human (22), Mouse (Murine) (6), Rat (Rattus) (2), Chicken (1), Cow (Bovine) (1), Pig (Porcine) (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (25), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunocytochemistry (ICC) (6), Immunofluorescence (IF) (6), Immunohistochemistry (IHC) (6), Immunoprecipitation (IP) (6), Chromatin Immunoprecipitation (ChiP) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunoassay (IA) (1)|
|Epitopes||Internal Region (2), N-Term (2), AA 1-14 (1), AA 271-446 (1), AA 403-417 (1)|