DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) antibody

Antigen: DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B)

Synonyms: cb633, dnmt3b, MGC152725, DNMT3B, LOC100218113, ICF, M.HsaIIIB

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN251114

Additional Information

Alternative name: DNMT3B

Gene ID: 1789

Immunogen: This antibody was raised against residues 4-101 of the human DNMT3b protein.

Cross-Reactivity: Human, Mouse (Murine)

Format: Serum

Description: Inactivation of tumor suppressor genes is a key component of the development of allcommon forms of human cancers. Aberrant methylation may contribute to many of thechanges, both physiological and pathological, associated with aging, including tumordevelopment. Hypermethylation of CpG island-containing promoters is associated withthe inactivation of genes important to tumor angiogenesis and progression. DNAmethytransferase (DNMT3b) is necessary for the maintenance of the methylationprocess. Alternate Names: anti-ICF antibody, anti-M.HsaIIIB antibody.

Specificity: This antibody is specific for DNMT3b. Species Reactivity: ABIN151734 reacts with human and mouse proteins. Other species have not been tested.

Application Details

Application Notes: Suggested working dilutions: immunoprecipitation 1:1,000,Western Blot 1:1,000. Positive Controls: HCT116 cell lysates.

Purification: Serum

Buffer: Preservative: No preservative.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Images

Western blot analysis using ABIN151734 to detect overexpressed DNMT-38 in HCT116 cells. Lane 1: 40 ug of nuclear extract from untransfected T24 cells. Lane 2-3: 40 ug of nuclear extracts transiently transfected to overexpress DNMT3B2 and DNMT3B3, respectively.

Publications

Rhee, Bachman, Park et al.: "DNMT1 and DNMT3b cooperate to silence genes in human cancer cells." in: Nature, Vol. 416, Issue 6880, pp. 552-6, 2002 (PubMed).

Beaulieu, Morin, Chute et al.: "An essential role for DNA methyltransferase DNMT3B in cancer cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28176-81, 2002 (PubMed).

Weisenberger, Velicescu, Preciado-Lopez et al.: "Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells." in: Gene, Vol. 298, Issue 1, pp. 91-9, 2002 (PubMed).

Robert, Morin, Beaulieu et al.: "DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells." in: Nature genetics, Vol. 33, Issue 1, pp. 61-5, 2003 (PubMed).

Xiong, Dowdy, Podratz et al.: "Histone deacetylase inhibitors decrease DNA methyltransferase-3B messenger RNA stability and down-regulate de novo DNA methyltransferase activity in human endometrial cells." in: Cancer research, Vol. 65, Issue 7, pp. 2684-9, 2005 (PubMed).

Morey, Smiraglia, James et al.: "DNA methylation pathway alterations in an autochthonous murine model of prostate cancer." in: Cancer research, Vol. 66, Issue 24, pp. 11659-67, 2006 (PubMed).

Morey Kinney, Smiraglia, James et al.: "Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model." in: Molecular cancer research : MCR, Vol. 6, Issue 8, pp. 1365-74, 2008 (PubMed).