PIP2 antibody

Details for Product No. ABIN251196
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Antigen
Reactivity
Human
(1), (1)
Host
Mouse
(3)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN251196
Quantity 0.1 mg
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Immunogen Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2.
Clone 2C11
Isotype IgM
Specificity Reacts with PtdIns(4,5)P2, Ptd(4)P and Ptd(3,4,5)P3.
Purification Protein A purified
Background Phosphatidylinositol 4,5-biphosphate (PIP2) has been implicated in a variety of cellularprocesses, including synaptic vesicle recycling. Alternate Names: anti-Phosphatidylinositol biphosphate antibody, anti-Phosphatidylinositol diphosphateantibody, anti-PIP 2 antibody.
Gene Symbol: PIP
Gene ID 5304
Research Area Signaling
Application Notes ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Neut: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Recommended dilutions: ELISA 1:100-1:2000, Immunoprecipitation 1:10-1:500, Western Blot 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:2000
Restrictions For Research Use only
Concentration 1.6 mg/mL
Buffer PBS
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
General Thomas, Steel, Prestwich et al.: "Generation of phosphatidylinositol-specific antibodies and their characterization." in: Biochemical Society transactions, Vol. 27, Issue 4, pp. 648-52, 2000 (PubMed).

Osborne, Thomas, Gschmeissner et al.: "Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing." in: Journal of cell science, Vol. 114, Issue Pt 13, pp. 2501-11, 2001 (PubMed).

Evans, Walker: "Endothelin-1 mobilizes profilin-1-bound PIP2 in cardiac muscle." in: Experimental biology and medicine (Maywood, N.J.), Vol. 231, Issue 6, pp. 882-7, 2006 (PubMed).

Hosts (3)
Reactivities (1), (1)
Applications (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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