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PIP2 antibody

Details for Product No. ABIN251196, Supplier: Log in to see
Antigen
Reactivity
Human
2
Host
Mouse
2
Clonality (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
2
1
1
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Immunogen Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2.
Clone 2C11
Isotype IgM
Specificity Reacts with PtdIns(4,5)P2, Ptd(4)P and Ptd(3,4,5)P3.
Purification Protein A purified
Background Phosphatidylinositol 4,5-biphosphate (PIP2) has been implicated in a variety of cellularprocesses, including synaptic vesicle recycling. Alternate Names: anti-Phosphatidylinositol biphosphate antibody, anti-Phosphatidylinositol diphosphateantibody, anti-PIP 2 antibody.
Gene Symbol: PIP
Gene ID 5304
Research Area Signaling
Application Notes ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Neut: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Recommended dilutions: ELISA 1:100-1:2000, Immunoprecipitation 1:10-1:500, Western Blot 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:2000
Restrictions For Research Use only
Concentration 1.6 mg/mL
Buffer PBS
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Product cited in: Evans, Walker: "Endothelin-1 mobilizes profilin-1-bound PIP2 in cardiac muscle." in: Experimental biology and medicine (Maywood, N.J.), Vol. 231, Issue 6, pp. 882-7, 2006 (PubMed).

Thomas, Steel, Prestwich et al.: "Generation of phosphatidylinositol-specific antibodies and their characterization." in: Biochemical Society transactions, Vol. 27, Issue 4, pp. 648-52, 2000 (PubMed).

Background publications Osborne, Thomas, Gschmeissner et al.: "Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing." in: Journal of cell science, Vol. 114, Issue Pt 13, pp. 2501-11, 2001 (PubMed).