Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Details for Product No. ABIN251414, Supplier: Log in to see
Antigen
  • hif1a
  • AA959795
  • HIF1alpha
  • MOP1
  • bHLHe78
  • HIF-1A
  • HIF-1alpha
  • HIF1
  • HIF1-ALPHA
  • PASD8
  • HIF1-alpha
  • hif-1a
  • hypoxia inducible factor 1 alpha
  • hypoxia inducible factor 1, alpha subunit
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • hypoxia-inducible factor 1 alpha
  • hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • Protein HIF-1
  • hif-1a
  • Hif1a
  • HIF1A
  • hif-1
Alternatives
anti-Human Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) antibody for ChIP DNA-Sequencing
Reactivity
Human
608
304
251
145
91
76
66
64
58
54
44
36
28
21
20
19
19
18
17
9
3
2
2
2
1
1
1
1
1
Host
Mouse
382
278
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
52
33
33
26
25
25
18
18
15
14
12
12
6
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
510
224
204
181
171
169
114
91
63
16
16
13
13
13
10
7
6
5
4
3
2
2
2
1
1
Options
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Immunogen Human HIF-1 alpha, corresponding to amino acids 329 - 530 [UniProt# Q16665].
Clone HA111
Isotype IgG2a
Purification Protein G purified
Alternative Name HIF-1 alpha (HIF1A Antibody Abstract)
Background Gene Symbol: HIF1A
Molecular Weight Theoretical MW: 93 kDa
Gene ID 3091
UniProt Q16665
Pathways Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Application Notes Western Blot 0.5 - 1.0 mg/mL, Immunohistochemistry 1:100, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:100IP usage was reported in scientific literature (PMID: 18222538). In IHC-P, staining was observed in the nucleus of human ovarian cancer tumor. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. **Nuclear extracts should be used for WB analysis.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-Paraffin Embedded Sections Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 degrees C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody (ABIN251414) Figure 1 illustrates Westerm blot detection of HIF-1 alpha (125-130 kDa) from human ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody (ABIN251414) Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [ABIN151762] - HIF-1 anti...
Product cited in: Lee, Spata, Bayne, Buza, Durham, Allman, Vonderheide, Simon: "Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia." in: Cancer discovery, Vol. 6, Issue 3, pp. 256-69, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Lauzier, Robitaille, Chan, Giaccia, Richard: "(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors." in: Molecular pharmacology, Vol. 74, Issue 1, pp. 282-8, 2008 (PubMed).

Rajakumar, Michael, Daftary, Jeyabalan, Gilmour, Conrad: "Proteasomal activity in placentas from women with preeclampsia and intrauterine growth restriction: implications for expression of HIF-alpha proteins." in: Placenta, Vol. 29, Issue 3, pp. 290-9, 2008 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Gillespie, Whang, Ragel, Flynn, Kelly, Jensen: "Silencing of hypoxia inducible factor-1alpha by RNA interference attenuates human glioma cell growth in vivo." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 8, pp. 2441-8, 2007 (PubMed).

Zheng, Ruas, Cao, Salomons, Cao, Poellinger, Pereira: "Cell-type-specific regulation of degradation of hypoxia-inducible factor 1 alpha: role of subcellular compartmentalization." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4628-41, 2006 (PubMed). (Sample species: Rabbit). Further details: Western Blotting,Immunoprecipitation

Jensen, Ragel, Whang, Gillespie: "Inhibition of hypoxia inducible factor-1alpha (HIF-1alpha) decreases vascular endothelial growth factor (VEGF) secretion and tumor growth in malignant gliomas." in: Journal of neuro-oncology, Vol. 78, Issue 3, pp. 233-47, 2006 (PubMed).