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Tumor Protein P53 Binding Protein 1 (TP53BP1) (C-Term) antibody

Details for Product No. ABIN251421, Supplier: Login to see
Antigen
Alternatives
anti-Human Tumor Protein P53 Binding Protein 1 antibody for Western Blotting
Epitope
C-Term
24
14
13
10
8
8
7
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
140
57
37
1
Host
Rabbit
112
24
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
87
50
49
38
24
23
14
11
7
6
3
2
1
1
Supplier
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Immunogen The epitope recognized by this antibody maps to a region between residues 1925 and the C-terminus (residue 1972) of human 53BP1 (NP_005648.1)
Cross-Reactivity (Details) Human has been tested in both WB,ICC/IF, mouse has only been tested in ICC/IF. rat,reactivity reported in scientific literature (PMID: 24244353)
Purification affinity purified
Alternative Name TP53BP1 (TP53BP1 Antibody Abstract)
Background P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putativesubstrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes tothe presumptive sites of damage, specifically, double-strand breaks. This also suggestsa role in DNA repair, maintaining genomic stability. Alternate Names: anti-53BP1 antibody, anti-53BP-1 antibody, anti-TP53BP1 antibody, anti-Tumor Proteinp53 Binding Protein 1 antibody.
Gene Symbol: TP53BP1
Gene ID 7158
NCBI Accession NP_005648
Research Area Transcription Factors
Pathways DNA Damage Repair
Application Notes This 53BP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western Blot. Chromatin Immunoprecipitation and Immunohistochemistry-Frozen were reported in scientific literature.
Recommended dilutions: Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:10-1:500, Western Blot 1:2000-1:10000
Protocol Protocol specific for 53BP1 Antibody IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changesII. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation styleB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (C-Term) antibody (ABIN251421) Western Blot analysis of human 53BP1, using ABIN151771. Sample: Whole cell lysate (20...
Immunohistochemistry (IHC) image for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (C-Term) antibody (ABIN251421) Placenta, Villi 40X
Product cited in: Spardy, Duensing, Hoskins et al.: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008 (PubMed).

Solier, Sordet, Kohn et al.: "Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways." in: Molecular and cellular biology, Vol. 29, Issue 1, pp. 68-82, 2008 (PubMed).

Palm, Hockemeyer, Kibe et al.: "Functional dissection of human and mouse POT1 proteins." in: Molecular and cellular biology, Vol. 29, Issue 2, pp. 471-82, 2008 (PubMed).

Wirthner, Wrann, Balamurugan et al.: "Impaired DNA double-strand break repair contributes to chemoresistance in HIF-1 alpha-deficient mouse embryonic fibroblasts." in: Carcinogenesis, Vol. 29, Issue 12, pp. 2306-16, 2008 (PubMed).

Guirouilh-Barbat, Redon, Pommier: "Transcription-coupled DNA double-strand breaks are mediated via the nucleotide excision repair and the Mre11-Rad50-Nbs1 complex." in: Molecular biology of the cell, Vol. 19, Issue 9, pp. 3969-81, 2008 (PubMed).

Ibarra, Schwob, Muendez: "Excess MCM proteins protect human cells from replicative stress by licensing backup origins of replication." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 26, pp. 8956-61, 2008 (PubMed).

Rothkamm, Balroop, Shekhdar et al.: "Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure." in: Radiology, Vol. 242, Issue 1, pp. 244-51, 2006 (PubMed).

Silverman, Takai, Buonomo et al.: "Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint." in: Genes & development, Vol. 18, Issue 17, pp. 2108-19, 2004 (PubMed).