ATM antibody (Ataxia Telangiectasia Mutated) (pSer1981)

Details for Product anti-ATM Antibody No. ABIN251422, Supplier: Log in to see
Antigen
  • ATM
  • Atm
  • CG6535
  • Dmel\\CG6535
  • Tefu
  • atm
  • atm/tefu
  • dATM
  • tef
  • Xatm
  • at1
  • atdc
  • tel1
  • telo1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • ATE
  • TEL1
  • TELO1
  • AI256621
  • C030026E19Rik
  • telomere fusion
  • ataxia telangiectasia mutated
  • ataxia telangiectasia mutated (atm)
  • ataxia telangiectasia mutated homolog (human)
  • tefu
  • atm
  • ATM
  • EDI_100660
  • CpipJ_CPIJ001772
  • BDBG_08252
  • PAAG_02532
  • MCYG_05088
  • VDBG_06833
  • ACLA_015700
  • AaeL_AAEL014900
  • MGYG_07634
  • PGTG_14279
  • Atm
  • LOC100349299
Alternatives
anti-Rat (Rattus) ATM antibody for Chromatin Immunoprecipitation
Epitope
pSer1981
88
40
17
16
16
15
8
8
6
6
6
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
353
157
77
27
8
1
1
1
1
Host
Mouse
229
97
17
9
3
Clonality (Clone)
Monoclonal ()
Conjugate
This ATM antibody is un-conjugated
12
11
10
9
6
6
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
214
125
109
88
86
71
59
45
27
21
13
6
4
4
2
2
2
1
1
Options
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Immunogen Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]
Clone 10H11-E12
Isotype IgG1 kappa
No Cross-Reactivity Dog (Canine)
Purification Protein G purified
Alternative Name ATM (ATM Antibody Abstract)
Background Gene Symbol: ATM
Molecular Weight Theoretical MW: 370 kDa
Gene ID 472
UniProt Q13315
Research Area DNA/RNA, Cancer
Pathways p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
Application Notes Western Blot 1:1000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200This ATM [p Ser1981] (10H11.E12) antibody useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band at ~370 kDa can be seen. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse spleen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for ATM Kinase Antibody Western Blot Procedure1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute primary antibody to 1:1,000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted anti-ATM-kinase.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.
Restrictions For Research Use only
Format Liquid
Concentration 0.97 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (pSer1981) (ABIN251422) Western Blot analysis of ATM-kinase, using ABIN151772. Sample: Irradiated or peroxida...
Immunohistochemistry (IHC) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (pSer1981) (ABIN251422) Immunohistochemistry: ATM [p Ser1981] Antibody (10H11.E12) [ABIN151772] - ATM antibod...
Product cited in: Liu, Liu, Gao, Reske, Li, Wu, Yang, Chen, Luo, Xiao: "A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation." in: Cancer letters, Vol. 391, pp. 152-161, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." in: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Takai, Jenkinson, Kabir, Babul-Hirji, Najm-Tehrani, Chitayat, Crow, de Lange: "A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus." in: Genes & development, Vol. 30, Issue 7, pp. 812-26, 2016 (PubMed). (Sample species: Human).

Banerjee, Banerjee, Mandal: "Enhanced chemotherapeutic efficacy of apigenin liposomes in colorectal cancer based on flavone-membrane interactions." in: Journal of colloid and interface science, Vol. 491, pp. 98-110, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Yang, Shen, Oishi, Matteson, Tian, Goronzy, Weyand: "Restoring oxidant signaling suppresses proarthritogenic T cell effector functions in rheumatoid arthritis." in: Science translational medicine, Vol. 8, Issue 331, pp. 331ra38, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Liedtke, Biebernick, Radke, Stapelkamp, Coenen, Zaehres, Fritz, Kogler: "DNA damage response in neonatal and adult stromal cells compared with induced pluripotent stem cells." in: Stem cells translational medicine, Vol. 4, Issue 6, pp. 576-89, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Caserta, Egriboz, Wang, Martin, Koivisto, Pecót, Kladney, Shen, Shim, Pham, Karikomi, Mauntel, Majumder, Cuitino, Tang, Srivastava, Yu, Wallace, Mo, Park, Fernandez, Pilarski, La Perle, Rosol et al.: "Noncatalytic PTEN missense mutation predisposes to organ-selective cancer development in vivo. ..." in: Genes & development, Vol. 29, Issue 16, pp. 1707-20, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Sayin, Ibrahim, Larsson, Nilsson, Lindahl, Bergo: "Antioxidants accelerate lung cancer progression in mice." in: Science translational medicine, Vol. 6, Issue 221, pp. 221ra15, 2014 (PubMed). Further details: Western Blotting

Park, Jeon, Kim: "Selenoprotein W promotes cell cycle recovery from G2 arrest through the activation of CDC25B." in: Biochimica et biophysica acta, Vol. 1823, Issue 12, pp. 2217-26, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Someya, Sakata, Matsumoto, Tauchi, Kai, Hareyama, Fukushima: "Effects of depletion of dihydropyrimidine dehydrogenase on focus formation and RPA phosphorylation." in: Journal of radiation research, Vol. 53, Issue 2, pp. 250-6, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Guerra, Albihn, Tronnersjö, Yan, Guidi, Stenerlöw, Sterzenbach, Josenhans, Fox, Schauer, Thelestam, Larsson, Henriksson, Frisan: "Myc is required for activation of the ATM-dependent checkpoints in response to DNA damage." in: PLoS ONE, Vol. 5, Issue 1, pp. e8924, 2010 (PubMed). Further details: Western Blotting

Gastaldello, Hildebrand, Faridani, Callegari, Palmkvist, Di Guglielmo, Masucci: "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases." in: Nature cell biology, Vol. 12, Issue 4, pp. 351-61, 2010 (PubMed).

Caslini, Connelly, Serna, Broccoli, Hess: "MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription." in: Molecular and cellular biology, Vol. 29, Issue 16, pp. 4519-26, 2009 (PubMed).

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009 (PubMed).

Yalcin, Zhang, Luciano, Mungamuri, Marinkovic, Vercherat, Sarkar, Grisotto, Taneja, Ghaffari: "Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25692-705, 2008 (PubMed).

Spardy, Duensing, Hoskins, Wells, Duensing: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008 (PubMed).

Luo, Rosenke, Czornak, Fortunato: "Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection." in: Journal of virology, Vol. 81, Issue 4, pp. 1934-50, 2007 (PubMed).

Bakkenist, Drissi, Wu, Kastan, Dome: "Disappearance of the telomere dysfunction-induced stress response in fully senescent cells." in: Cancer research, Vol. 64, Issue 11, pp. 3748-52, 2004 (PubMed).

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003 (PubMed).