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BUB1B antibody (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast)) (N-Term)

Details for Product anti-BUB1B Antibody No. ABIN251432, Supplier: Log in to see
Antigen
  • 31/13
  • AU045529
  • BcDNA:LD23835
  • Bub1
  • bub1
  • BUB1
  • BUB1-like
  • Bub1A
  • bub1b
  • BUB1beta
  • bubr1
  • bubR1
  • BUBR1
  • BubR1
  • BubRI
  • CG7838
  • CG 7838
  • CG14030
  • CG14030/Bub1
  • dBUB1
  • DmBUB1
  • Dmbub1
  • Dmel\\CG7838
  • Dmel\\CG14030
  • hBUBR1
  • l(2)k03113
  • l(2)k06109
  • MAD3L
  • mcl(2)Z1525
  • MVA1
  • SSK1
Epitope
N-Term, AA 1-350
46
25
13
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
163
60
8
6
2
1
Host
Mouse
94
69
6
Clonality (Clone)
Monoclonal ()
Conjugate
This BUB1B antibody is un-conjugated
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
146
73
54
45
33
26
24
13
11
5
4
3
3
3
2
1
Supplier
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Immunogen A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350).
Clone 8G1
Isotype IgG1 kappa
Specificity NB100-353 is specific for hBUBR1. There should be no cross-reactivity with hBUB1.
Purification Protein G purified
Alternative Name BubR1 (BUB1B Antibody Abstract)
Background Compromised spindle checkpoints are thought to play an important role in geneticinstability that predisposes cells to malignant transformations. Human cells contain tworelated checkpoint protein kinases, evolved from the BUB1 gene, hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotickinetochore-microtubule interactions. hBUBR1 is essential for normal mitoticprogression as it prevents cells from entering into anaphase too early. BUBR1 hasbeen detected in human cancers. It has been identified as a novel binding partner ofBCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate theprogression of breast cancer by compromising the mitotic checkpoint control throughinactivation of BUBR1. Alternate Names: anti-BubR1 antibody, anti-budding uninhibited by benzimidazoles related 1 antibody,anti-Bub1b antibody, anti-budding uninhibited by benzimidazoles 1 homolog betaantibody, anti-MAD3L antibody, anti-mitotic arrest deficient-like 3 like antibody.
Gene Symbol: BUB1B
Gene ID 701, 12236
UniProt O60566
Research Area Cell Cycle
Application Notes This BubR1 (8G1) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot. By WB, this antibody recognizes a band at ~120 kDa.
Recommended dilutions: Flow Cytometry 1 µg per million cells, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Western Blot 1:2000-1:5000
Restrictions For Research Use only
Format Liquid
Concentration 0.6 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Western Blotting (WB) image for anti-BUB1B antibody (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast)) (N-Term) (ABIN251432) BUBR1 detected in HeLa cell lysate using ABIN151818.
Western Blotting (WB) image for anti-BUB1B antibody (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast)) (N-Term) (ABIN251432) Detection of BUBR1 in HeLa WCE using ABIN151818.
Immunofluorescence (IF) image for anti-BUB1B antibody (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast)) (N-Term) (ABIN251432) anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-350), (N-Term) antibody (Image 3)
Product cited in: Trachana, van Wely, Guerrero, Fütterer, Martínez-A: "Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 8, pp. 2691-6, 2007

Background publications Johnson, Scott, Holt, Hussein, Taylor: "Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression." in: Journal of cell science, Vol. 117, Issue Pt 8, pp. 1577-89, 2004

Chan, Jablonski, Sudakin, Hittle, Yen: "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC." in: The Journal of cell biology, Vol. 146, Issue 5, pp. 941-54, 1999