Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (N-Term), (AA 1-350) antibody

Details for Product No. ABIN251432
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Antigen
Synonyms
bub1b, BUB1, BUB1-like, BubR1, BubRI, CG14030, CG14030/Bub1, Dmel\\CG14030, bub1, 31/13, BUBR1, BcDNA:LD23835, Bub1, CG 7838, CG7838, DmBUB1, Dmbub1, Dmel\\CG7838, bubR1, bubr1, dBUB1, l(2)k03113, l(2 ... show more
bub1b, BUB1, BUB1-like, BubR1, BubRI, CG14030, CG14030/Bub1, Dmel\\CG14030, bub1, 31/13, BUBR1, BcDNA:LD23835, Bub1, CG 7838, CG7838, DmBUB1, Dmbub1, Dmel\\CG7838, bubR1, bubr1, dBUB1, l(2)k03113, l(2)k06109, mcl(2)Z1525, BUB1beta, Bub1A, MAD3L, MVA1, SSK1, hBUBR1, AU045529 show less
Epitope
N-Term, AA 1-350
(20), (9), (8), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(100), (46), (12), (7), (3), (1)
Host
Mouse
(51), (49), (9)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(93), (39), (30), (27), (25), (12), (10), (7), (7), (6), (2), (2), (2), (1)
Pubmed 3 references available
Quantity 0.025 mL
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Catalog No. ABIN251432
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Immunogen A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350).
Clone 8G1
Isotype IgG1 kappa
Specificity NB100-353 is specific for hBUBR1. There should be no cross-reactivity with hBUB1.
Purification Protein G purified
Alternative Name BUB1B
Background Compromised spindle checkpoints are thought to play an important role in geneticinstability that predisposes cells to malignant transformations. Human cells contain tworelated checkpoint protein kinases, evolved from the BUB1 gene, hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotickinetochore-microtubule interactions. hBUBR1 is essential for normal mitoticprogression as it prevents cells from entering into anaphase too early. BUBR1 hasbeen detected in human cancers. It has been identified as a novel binding partner ofBCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate theprogression of breast cancer by compromising the mitotic checkpoint control throughinactivation of BUBR1. Alternate Names: anti-BubR1 antibody, anti-budding uninhibited by benzimidazoles related 1 antibody,anti-Bub1b antibody, anti-budding uninhibited by benzimidazoles 1 homolog betaantibody, anti-MAD3L antibody, anti-mitotic arrest deficient-like 3 like antibody.
Gene Symbol: BUB1B
Gene ID 701
UniProt O60566
Research Area Cell Cycle
Application Notes This BubR1 (8G1) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot. By WB, this antibody recognizes a band at ~120 kDa.
Recommended dilutions: Flow Cytometry 1 µg per million cells, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Western Blot 1:2000-1:5000
Restrictions For Research Use only
Format Liquid
Concentration 0.6 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (N-Term), (AA 1-350) antibody BUBR1 detected in HeLa cell lysate using ABIN151818.
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (N-Term), (AA 1-350) antibody (2) Detection of BUBR1 in HeLa WCE using ABIN151818.
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (N-Term), (AA 1-350) antibody (3) Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with ABIN151818. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK
General Chan, Jablonski, Sudakin et al.: "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC." in: The Journal of cell biology, Vol. 146, Issue 5, pp. 941-54, 1999 (PubMed).

Johnson, Scott, Holt et al.: "Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression." in: Journal of cell science, Vol. 117, Issue Pt 8, pp. 1577-89, 2004 (PubMed).

Trachana, van Wely, Guerrero et al.: "Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 8, pp. 2691-6, 2007 (PubMed).

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