Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibody

Details for Product No. ABIN251432
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Antigen
Synonyms
BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1, AU045529, bub1b, BUB1, BUB1-like, BubR1, BubRI, CG14030, CG14030/Bub1, Dmel\\CG14030, bub1, 31/13, BcDNA:LD23835, Bub1, CG 7838, CG7838, DmBUB1, Dmbu ... show more
BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1, AU045529, bub1b, BUB1, BUB1-like, BubR1, BubRI, CG14030, CG14030/Bub1, Dmel\\CG14030, bub1, 31/13, BcDNA:LD23835, Bub1, CG 7838, CG7838, DmBUB1, Dmbub1, Dmel\\CG7838, bubR1, bubr1, dBUB1, l(2)k03113, l(2)k06109, mcl(2)Z1525 show less
Reactivity
Human, Mouse (Murine)
(88), (43), (12), (6), (3), (1)
Host
Mouse
(49), (39), (9)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(82), (37), (26), (25), (18), (10), (10), (7), (7), (6), (2), (2), (2), (1)
Pubmed 3 references available
Catalog no. ABIN251432
Quantity 25 µL
Price
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Immunogen A portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1-350 of humanBUBR1).
Clone 8G1
Isotype IgG1
Specificity ABIN151818 is specific for hBUBR1. There should be no cross-reactivity with hBUB1. Species Reactivity: Human and mouse. Other species have not been tested. Localization: Nuclear.
Cross-Reactivity Human, Mouse (Murine)
Purification Purified
Alternative Name BUB1B
Background Compromised spindle checkpoints are thought to play an important role in geneticinstability that predisposes cells to malignant transformations. Human cells contain tworelated checkpoint protein kinases, evolved from the BUB1 gene, hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotickinetochore-microtubule interactions. hBUBR1 is essential for normal mitoticprogression as it prevents cells from entering into anaphase too early. BUBR1 hasbeen detected in human cancers. It has been identified as a novel binding partner ofBCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate theprogression of breast cancer by compromising the mitotic checkpoint control throughinactivation of BUBR1. Alternate Names: anti-BubR1 antibody, anti-budding uninhibited by benzimidazoles related 1 antibody,anti-Bub1b antibody, anti-budding uninhibited by benzimidazoles 1 homolog betaantibody, anti-MAD3L antibody, anti-mitotic arrest deficient-like 3 like antibody.
Gene ID 701
Research Area Cell Cycle
Application Notes Suggested working dilutions: immunofluorescence 1:100,Western Blot 1:2,000-1:5,000
Restrictions For Research Use only
Format Liquid
Concentration 0.6 mg/ml
Buffer Preservative: Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibody BUBR1 detected in HeLa cell lysate using ABIN151818.
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibody (2) Detection of BUBR1 in HeLa WCE using ABIN151818.
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibody (3) Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with ABIN151818. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK
General Chan, Jablonski, Sudakin et al.: "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC." in: The Journal of cell biology, Vol. 146, Issue 5, pp. 941-54, 1999 (PubMed).

Johnson, Scott, Holt et al.: "Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression." in: Journal of cell science, Vol. 117, Issue Pt 8, pp. 1577-89, 2004 (PubMed).

Trachana, van Wely, Guerrero et al.: "Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 8, pp. 2691-6, 2007 (PubMed).

Hosts (49), (39), (9)
Reactivities (88), (43), (12), (6), (3), (1)
Applications (82), (37), (26), (25), (18), (10), (10), (7), (7), (6), (2), (2), (2), (1)
Conjugates (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (14), (9), (7), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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