Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) antibody

Antigen: Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B)

Synonyms: SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta, Bub1b, BUB1B, bub1b, Bub1a, C80208, D2Xrf87, AL022991, AU045529, MGC36092, BUB1, BUB1-like, BubR1, CG14030/Bub1, DmelCG14030, CG14030, 31/13, BcDNA:LD23835, CG 7838, DmBUB1, bubR1, dBUB1, l(2)k03113, l(2)k06109, DmelCG7838, CG7838

Clonality: Monoclonal (8G1)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN251432

Additional Information

Alternative name: BUB1B

Gene ID: 701

Immunogen: A portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1-350 of humanBUBR1).

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1

Clone: 8G1

Description: Compromised spindle checkpoints are thought to play an important role in geneticinstability that predisposes cells to malignant transformations. Human cells contain tworelated checkpoint protein kinases, evolved from the BUB1 gene, hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotickinetochore-microtubule interactions. hBUBR1 is essential for normal mitoticprogression as it prevents cells from entering into anaphase too early. BUBR1 hasbeen detected in human cancers. It has been identified as a novel binding partner ofBCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate theprogression of breast cancer by compromising the mitotic checkpoint control throughinactivation of BUBR1. Alternate Names: anti-BubR1 antibody, anti-budding uninhibited by benzimidazoles related 1 antibody,anti-Bub1b antibody, anti-budding uninhibited by benzimidazoles 1 homolog betaantibody, anti-MAD3L antibody, anti-mitotic arrest deficient-like 3 like antibody.

Specificity: ABIN151818 is specific for hBUBR1. There should be no cross-reactivity with hBUB1. Species Reactivity: Human and mouse. Other species have not been tested. Localization: Nuclear.

Synonyms: SSK1, BUBR1, Bub1A, MAD3L, hBUBR1, BUB1beta, Bub1b, BUB1B, bub1b, Bub1a, C80208, D2Xrf87, AL022991, AU045529, MGC36092, BUB1, BUB1-like, BubR1, CG14030/Bub1, DmelCG14030, CG14030, 31/13, BcDNA:LD23835, CG 7838, DmBUB1, bubR1, dBUB1, l(2)k03113, l(2)k06109, DmelCG7838, CG7838

Application Details

Application Notes: Suggested working dilutions: immunofluorescence 1:100,Western Blot 1:2,000-1:5,000

Concentration: 0.6 mg/ml

Purification: Purified

Buffer: Preservative: Sodium Azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Cell Cycle

Restrictions: For Research Use only

Images

BUBR1 detected in HeLa cell lysate using ABIN151818.

Detection of BUBR1 in HeLa WCE using ABIN151818.

Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with ABIN151818. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK

Publications

Chan, Jablonski, Sudakin et al.: "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC." in: The Journal of cell biology, Vol. 146, Issue 5, pp. 941-54, 1999 (PubMed).

Johnson, Scott, Holt et al.: "Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression." in: Journal of cell science, Vol. 117, Issue Pt 8, pp. 1577-89, 2004 (PubMed).

Trachana, van Wely, Guerrero et al.: "Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 8, pp. 2691-6, 2007 (PubMed).