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Solute Carrier Family 31 (Copper Transporters), Member 1 (SLC31A1) (C-Term) antibody

Details for Product No. ABIN251444, Supplier: Log in to see
Antigen
  • 4930445G01Rik
  • AI787263
  • AU016967
  • copper transporter 1
  • COPT1
  • copt1
  • ctr-1
  • ctr1
  • CTR1
  • Ctr1
  • hctr1
  • K19M22.24
  • LRRGT00200
  • Slc31a1
  • SLC31A1
  • Xctr1
  • zgc:76839
Epitope
C-Term
21
4
4
2
2
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
65
19
16
15
15
14
4
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
38
32
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
60
52
50
6
6
6
4
Supplier
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Immunogen A synthetic peptide derived from a C-terminal sequence of human SLC31A1/CTR1 [UniProt# O15431]
Purification Immunogen affinity purified
Alternative Name SLC31A1/CTR1 (SLC31A1 Antibody Abstract)
Background Gene Symbol: SLC31A1
UniProt O15431
Application Notes Western Blot, Immunohistochemistry 1:250, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry-Paraffin 1:250, Immunohistochemistry-Frozen
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-paraffin protocol specific for SLC31A1 / CTR1 Antibody Immunohistochemistry Procedure
1. Cut 5 mm tissue sections and mount on a slide.
. Deparaffinize formalin-fixed and paraffin-embedded tissue sections with three 10 min soaks in xylene, and then rehydrate with two 5 min soaks in 100 % ethanol, two 5 minute soaks in 95 % ethanol and two 5 minute soaks in 70 % ethanol followed by a rinse (10 dips) in 1X PBS.
. Block endogenous peroxidases with 0.3 % H2O2 in PBS for 30 minutes followed by 3 rinses in 1X PBS.Perform antigen retrieval:
. Microwave in citrate buffer pH 5 for 5 minutes. Check buffer level and microwave again in citrate buffer pH 5 for another 5 minutes. Allow the slides to cool for 20 min and rinse 3X in 0.1 % Trition-X in PBS.
. Citrate buffer formula: 0.1M citric acid stock (21.01 g of citric acid in 1 L water) and 0.1M sodium citrate stock (29.41g of sodium citrate in 1 L water). Mix 9 mLs of citric acid stock with 41 mLs of sodium citrate stock and complete to 500 mL with distilled water.(Note: citrate buffer is now available for purchase from several companies including BD Pharmingen and Biogenx).Perform immunohistochemical staining according to the guidelines of the Catalyzed Signal Amplification System (DAKO, Carpinteria, CA, cat# K1500):
. Block non-specific protein binding by setting slides in chamber with 1 % BSA in PBS and incubating for 20 minutes.
. For amplification, incubate with CSA kit Protein block (solution #2) for 5 minutes.
. Incubate the slides with a 1:250 dilution of anti-hCTR1 antibody , diluted in 1 % BSA in PBS and incubate overnight at 4C.
. Wash slides with 3X TBS/0.1 % Tween.
. Apply Rabbit Link antibody (from DAKO, Carpinteria, CA, Cat # K1498 ) for 1 hour, followed by 3X wash with TBS/0.1 % Tween.
. Apply prepared streptavidin complex (from DAKO CSA kit, solutions #5,6,7) for 15 minutes, followed by 3X wash with TBS/0.1 % Tween.(Note: the prepared streptavidin complex needs to be made fresh and needs to sit for 30 minutes prior to use).
. Apply amplification reagent (from DAKO CSA kit, solution #8) for 15 minutes, followed by 3X wash with TBS/0.1 % Tween.
. Apply streptavidin peroxidase (from DAKO CSA kit, solution #9) for 15 minutes, followed by 3X wash with TBS/0.1 % Tween.
. Incubate with substrate: prepare fresh AEC (3-amino-9ethylcarbazol) (Vector Laboratories, Burlingame, California) and apply for 10-30 minutes followed by 3X wash with PBS.
. Counterstain with Mayers Hematoxylin for 2 minutes followed by 3X wash with water.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/phosphate, pH 7-8
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Immunohistochemistry (IHC) image for anti-Solute Carrier Family 31 (Copper Transporters), Member 1 (SLC31A1) (C-Term) antibody (ABIN251444) The photo on the left is antibody staining of hCTR1 overexpressing cell line. The ph...
Immunohistochemistry (IHC) image for anti-Solute Carrier Family 31 (Copper Transporters), Member 1 (SLC31A1) (C-Term) antibody (ABIN251444) Panel 1: human CTR1 staining of breast cancer tissue. Panel 2: human CTR1-antigen com...
Product cited in: Doherty, Lawlor, Gillet, Gottesman, OLeary, Stordal: "Collateral sensitivity to cisplatin in KB-8-5-11 drug-resistant cancer cells." in: Anticancer research, Vol. 34, Issue 1, pp. 503-7, 2014 (PubMed). (Sample species: Human).

Liu, Kim, Yan, Ding, Ip, Jong, Mercer, McKeage: "Contributions of rat Ctr1 to the uptake and toxicity of copper and platinum anticancer drugs in dorsal root ganglion neurons." in: Biochemical pharmacology, Vol. 85, Issue 2, pp. 207-15, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Ip, Liu, McKeage: "Evaluation of effects of copper histidine on copper transporter 1-mediated accumulation of platinum and oxaliplatin-induced neurotoxicity in vitro and in vivo." in: Clinical and experimental pharmacology & physiology, Vol. 40, Issue 6, pp. 371-8, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Yoshida, Teramae, Yamauchi, Fukuda, Yasui, Sumi, Honda, Ishiko: "Association of copper transporter expression with platinum resistance in epithelial ovarian cancer." in: Anticancer research, Vol. 33, Issue 4, pp. 1409-14, 2013 (PubMed).

Stordal, Hamon, McEneaney, Roche, Gillet, OLeary, Gottesman, Clynes: "Resistance to paclitaxel in a cisplatin-resistant ovarian cancer cell line is mediated by P-glycoprotein." in: PLoS ONE, Vol. 7, Issue 7, pp. e40717, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Chen, Feng, Lin, Zhang, Li, Liang, Li: "Penicillamine increases free copper and enhances oxidative stress in the brain of toxic milk mice." in: PLoS ONE, Vol. 7, Issue 5, pp. e37709, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry (Frozen Sections),Immunohistochemistry

Pourvali, Matak, Latunde-Dada, Solomou, Mastrogiannaki, Peyssonnaux, Sharp: "Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2α." in: FEBS letters, Vol. 586, Issue 16, pp. 2423-7, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

More, Akil, Ianculescu, Geier, Lustig, Giacomini: "Role of the copper transporter, CTR1, in platinum-induced ototoxicity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 28, pp. 9500-9, 2010 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry (Frozen Sections)

Jandial, Farshchi-Heydari, Larson, Elliott, Wrasidlo, Howell: "Enhanced delivery of cisplatin to intraperitoneal ovarian carcinomas mediated by the effects of bortezomib on the human copper transporter 1." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 2, pp. 553-60, 2009 (PubMed).

Holzer, Howell: "The internalization and degradation of human copper transporter 1 following cisplatin exposure." in: Cancer research, Vol. 66, Issue 22, pp. 10944-52, 2006 (PubMed).

Holzer, Varki, Le, Gibson, Naredi, Howell: "Expression of the human copper influx transporter 1 in normal and malignant human tissues." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 9, pp. 1041-9, 2006 (PubMed).

Peng, Lu, Janisse, Muzik, Shields: "PET of human prostate cancer xenografts in mice with increased uptake of 64CuCl2." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 47, Issue 10, pp. 1649-52, 2006 (PubMed).

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