You are viewing an incomplete version of our website. Please click to reload the website as full version.

CCR5 antibody (C-C Chemokine Receptor Type 5)

Details for Product anti-CCR5 Antibody No. ABIN251663, Supplier: Log in to see
Antigen
  • AM4-7
  • CC-CKR-5
  • CCCKR5
  • CCR-5
  • CCR5
  • CD195
  • CKR-5
  • CKR5
  • Ckr5
  • CMKBR5
  • Cmkbr5
  • GPCR
  • IDDM22
Alternatives
anti-Human CCR5 antibody for Flow Cytometry
Reactivity
Human, Primate
428
76
50
13
8
2
1
1
1
Host
Rabbit
197
177
63
17
13
2
Clonality
Polyclonal
Conjugate
This CCR5 antibody is un-conjugated
43
28
15
14
14
12
11
8
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), In vitro Assay (in vitro), ELISA, Western Blotting (WB)
270
211
150
64
53
52
46
33
31
17
12
11
8
5
4
4
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen A synthetic peptide demonstrating a high affinity towards the monoclonal CCR5 2D7. This peptide is known as the 2D7-2SK peptide and consists of the following residues: EWQKEGLVTLWLGGGGSC.
Specificity This antibody is specific for the linear epitope detected by the monoclonal antibody 2D7.
Purification Immunogen affinity purified
Alternative Name CCR5 (CCR5 Antibody Abstract)
Background Gene Symbol: CCR5
Gene ID 1234
Research Area Signaling, Receptors, Cardiovascular, Alzheimer's Disease, Atherosclerosis, Innate Immunity, CD Antigens, Chemokines
Pathways TCR Signaling
Application Notes Western Blot 1.0 μg/mL, Flow Cytometry 25 μg/mL, ELISA 1 μg/mL, Immunocytochemistry/Immunofluorescence 1:100, Functional, In vitro assay
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Flow Cytometry protocol specific for CCR5 Antibody Flow Cytometry
1. CEM.NKR.CCR5 cells were stained with 25 µg/ml anti-CCR5 or with preimmune rabbit IgG for 60 minutes on ice.
. This was followed by extensive washing and staining with a FITC-conjugated goat anti-rabbit secondary antibody.
. Flow cytometry was performed and analyzed with Cell Quest Software on FACSCalibur (BD Biosciences).Western blot protocol specific for CCR5 Antibody Western Blot Protocol
. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunocytochemistry (ICC) image for anti-CCR5 antibody (C-C Chemokine Receptor Type 5) (ABIN251663) anti-C-C Chemokine Receptor Type 5 (CCR5) antibody
Product cited in: Khurana, Kennedy, King, Golding: "Identification of a linear peptide recognized by monoclonal antibody 2D7 capable of generating CCR5-specific antibodies with human immunodeficiency virus-neutralizing activity." in: Journal of virology, Vol. 79, Issue 11, pp. 6791-800, 2005 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Background publications Trkola, Matthews, Gordon, Ketas, Moore: "A cell line-based neutralization assay for primary human immunodeficiency virus type 1 isolates that use either the CCR5 or the CXCR4 coreceptor." in: Journal of virology, Vol. 73, Issue 11, pp. 8966-74, 1999 (PubMed).