Did you know that you can buy products from over 140 different suppliers from us?

Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody

Details for Product No. ABIN252473
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
AI132552, IKK-2, IKK-beta, IKK2, IKK[b], IKKbeta, AIM-1, IKKB, NFKBIKB, ikk-beta, ikk2, ikkb, ikkbeta, IKBKB, CG4201, DIK, DLAK, DmIKK, DmIKKBeta, DmIKKb, DmIKKbeta, DmIkkbeta, Dmel\\CG4201, Dmikkb, D ... show more
AI132552, IKK-2, IKK-beta, IKK2, IKK[b], IKKbeta, AIM-1, IKKB, NFKBIKB, ikk-beta, ikk2, ikkb, ikkbeta, IKBKB, CG4201, DIK, DLAK, DmIKK, DmIKKBeta, DmIKKb, DmIKKbeta, DmIkkbeta, Dmel\\CG4201, Dmikkb, Dmikkbeta, IK, IKK, IKKb, IRD-5, IRD5, Ik, Ikkb, Ikkbeta, Ird-5, Ird5, LRK, Lak, anon-89Bd, dIKK, dIKK-beta, dIKKbeta, dLak, dmIKKbeta, ikk, ird, ird5/DmIkk[beta] show less
Reactivity
Human, Mouse (Murine)
(420), (151), (139), (98), (98), (86), (49)
Host
Mouse
(376), (50), (6), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(26), (26), (26), (16), (16), (16), (10), (10), (10), (10), (10), (10), (10), (10)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(196), (179), (126), (100), (55), (51), (33), (20), (17), (4), (3), (2), (1)
Pubmed 13 references available
Quantity 0.1 mg
Shipping to United States (Change)
Catalog No. ABIN252473
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
Clone 10AG2
Isotype IgG1
Specificity An approx. 87 kDa band should be observed.
Purification Protein G purified
Alternative Name IKBKB / IKKB
Background NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitoryproteins that mask the nuclear localization signal of NF-kB thereby preventingtranslocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factoror other cytokines results in phosphorylation and degradation of IkB releasing NF-kBdimers. NF-kB dimer subsequently translocates to the nucleus and activates targetgenes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkBkinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5).IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAPkinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown thatIKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption ofIKK2/b gene in mice also results in extensive liver damage from apotopsis and death asembryo (9). Alternate Names: anti-I kappa B kinase 2 antibody, anti-I kappa B kinase beta antibody, anti-IkBKBantibody, anti-IKK 2 antibody, anti-IKK B antibody, anti-IKK2 antibody, anti-IKKBantibody, anti-Inhibitor of kappa light chain gene enhancer in B cells antibody,anti-Inhibitor of kappa light polypeptide gene enhancer in B cells antibody, anti-Inhibitorof kappa light polypeptide gene enhancer in B cells kinase beta antibody, anti-Inhibitorof nuclear factor kappa B kinase beta subunit antibody, anti-Inhibitor of nuclear factorkappa B kinase subunit beta antibody, anti-MGC131801 antibody, anti-NFKBIKBantibody, anti-Nuclear factor NF kappa B inhibitor kinase beta antibody, anti-Nuclearfactor of kappa light chain gene enhancer in B cells inhibitor antibody.
Gene Symbol: IKBKB
Gene ID 3551
NCBI Accession NP_001547
Application Notes Useful in Immunohistochemistry-Paraffin See Page et al.
Recommended dilutions: Flow Cytometry 0.25-0.5 µg/ 10^6 cells, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunoprecipitation 1:10-1:500, Western Blot 2-4 µg/mL
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody Western blot analysis of 30 µg of total cell lysate from A) Daudi, B) HeLa, and C) mouse NIH 3T3 cells using ABIN252473 at 2 ug/ml dilution.
General Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Lee, Hagler, Chen et al.: "Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway." in: Cell, Vol. 88, Issue 2, pp. 213-22, 1997 (PubMed).

Malinin, Boldin, Kovalenko et al.: "MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1." in: Nature, Vol. 385, Issue 6616, pp. 540-4, 1997 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Delhase, Hayakawa, Chen et al.: "Positive and negative regulation of IkappaB kinase activity through IKKbeta subunit phosphorylation." in: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 309-13, 1999 (PubMed).

Li, Van Antwerp, Mercurio et al.: "Severe liver degeneration in mice lacking the IkappaB kinase 2 gene." in: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 321-5, 1999 (PubMed).

Majumdar, Aggarwal: "Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2911-20, 2001 (PubMed).

Anto, Mukhopadhyay, Shishodia et al.: "Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2." in: Carcinogenesis, Vol. 23, Issue 9, pp. 1511-8, 2002 (PubMed).

Ashikawa, Majumdar, Banerjee et al.: "Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 11, pp. 6490-7, 2002 (PubMed).

Santos, Perez, Segrelles et al.: "Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 13422-30, 2003 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN252473
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
Did you look for something else?
back to top