Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB)

Synonyms: IKK2, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801, ikk2, ikkb, ikkbeta, MGC80376, ikk-beta, IKBKB, DIK, DLAK, DmIKK, DmIKKBeta, DmIKKb, IK, IKK, IKKbeta, Ird-5, Ird5, LRK, Lak, anon-89Bd, dIKK, dIKK-beta, ird, ird5/DmIkk[beta], DmelCG4201, CG4201

Clonality: Monoclonal (10AG2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN252473

Additional Information

Alternative name: IKBKB / IKKB

Gene ID: 3551

Immunogen: A full-length human IKKb recombinant protein was used to immunize mice.

Cross-Reactivity: Human

Isotype: IgG1

Clone: 10AG2

Description: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitoryproteins that mask the nuclear localization signal of NF-kB thereby preventingtranslocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factoror other cytokines results in phosphorylation and degradation of IkB releasing NF-kBdimers. NF-kB dimer subsequently translocates to the nucleus and activates targetgenes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkBkinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5).IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAPkinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown thatIKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption ofIKK2/b gene in mice also results in extensive liver damage from apotopsis and death asembryo (9). Alternate Names: anti-I kappa B kinase 2 antibody, anti-I kappa B kinase beta antibody, anti-IkBKBantibody, anti-IKK 2 antibody, anti-IKK B antibody, anti-IKK2 antibody, anti-IKKBantibody, anti-Inhibitor of kappa light chain gene enhancer in B cells antibody,anti-Inhibitor of kappa light polypeptide gene enhancer in B cells antibody, anti-Inhibitorof kappa light polypeptide gene enhancer in B cells kinase beta antibody, anti-Inhibitorof nuclear factor kappa B kinase beta subunit antibody, anti-Inhibitor of nuclear factorkappa B kinase subunit beta antibody, anti-MGC131801 antibody, anti-NFKBIKBantibody, anti-Nuclear factor NF kappa B inhibitor kinase beta antibody, anti-Nuclearfactor of kappa light chain gene enhancer in B cells inhibitor antibody.

Specificity: An approx. 87 kDa band should be observed. Species Reactivity: Cross reacts with Human.

Synonyms: IKK2, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801, ikk2, ikkb, ikkbeta, MGC80376, ikk-beta, IKBKB, DIK, DLAK, DmIKK, DmIKKBeta, DmIKKb, IK, IKK, IKKbeta, Ird-5, Ird5, LRK, Lak, anon-89Bd, dIKK, dIKK-beta, ird, ird5/DmIkk[beta], DmelCG4201, CG4201

Application Details

Application Notes: Suggested working dilutions: Western Blot 2-4 ug/ml. Positive Controls: Daudi, HeLa, NIH 3T3

Purification: Aff - Purified

Buffer: PBS containing 0.2% gelatin. Preservative: 0.05 % Sodium Azide

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Images

Western blot analysis of 30 µg of total cell lysate from A) Daudi, B) HeLa, and C) mouse NIH 3T3 cells using ABIN252473 at 2 ug/ml dilution.

Publications

Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Lee, Hagler, Chen et al.: "Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway." in: Cell, Vol. 88, Issue 2, pp. 213-22, 1997 (PubMed).

Malinin, Boldin, Kovalenko et al.: "MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1." in: Nature, Vol. 385, Issue 6616, pp. 540-4, 1997 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Delhase, Hayakawa, Chen et al.: "Positive and negative regulation of IkappaB kinase activity through IKKbeta subunit phosphorylation." in: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 309-13, 1999 (PubMed).

Li, Van Antwerp, Mercurio et al.: "Severe liver degeneration in mice lacking the IkappaB kinase 2 gene." in: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 321-5, 1999 (PubMed).

Majumdar, Aggarwal: "Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2911-20, 2001 (PubMed).

Anto, Mukhopadhyay, Shishodia et al.: "Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2." in: Carcinogenesis, Vol. 23, Issue 9, pp. 1511-8, 2002 (PubMed).

Ashikawa, Majumdar, Banerjee et al.: "Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 11, pp. 6490-7, 2002 (PubMed).

Santos, Perez, Segrelles et al.: "Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 13422-30, 2003 (PubMed).