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IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta)

Details for Product anti-IKBKB Antibody No. ABIN252473, Supplier: Login to see New
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Human, Mouse (Murine)
(608), (152), (122), (14), (8), (2), (2), (1), (1)
(550), (72), (9), (2)
Clonality (Clone)
Monoclonal ()
This IKBKB antibody is un-conjugated
(36), (36), (36), (31), (31), (31), (9), (5), (5), (5), (5), (5), (5), (5), (5)
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(297), (265), (238), (83), (62), (46), (45), (24), (15), (6), (5), (4), (3), (3), (1), (1)
Pubmed 13 references available
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Quantity 0.1 mg
Shipping to United States ( )
Immunogen A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
Clone 10AG2
Isotype IgG1
Specificity An approx. 87 kDa band should be observed.
Purification Protein G purified
Alternative Name IKBKB / IKKB (IKBKB Antibody Abstract)
Background NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitoryproteins that mask the nuclear localization signal of NF-kB thereby preventingtranslocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factoror other cytokines results in phosphorylation and degradation of IkB releasing NF-kBdimers. NF-kB dimer subsequently translocates to the nucleus and activates targetgenes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkBkinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5).IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAPkinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown thatIKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption ofIKK2/b gene in mice also results in extensive liver damage from apotopsis and death asembryo (9). Alternate Names: anti-I kappa B kinase 2 antibody, anti-I kappa B kinase beta antibody, anti-IkBKBantibody, anti-IKK 2 antibody, anti-IKK B antibody, anti-IKK2 antibody, anti-IKKBantibody, anti-Inhibitor of kappa light chain gene enhancer in B cells antibody,anti-Inhibitor of kappa light polypeptide gene enhancer in B cells antibody, anti-Inhibitorof kappa light polypeptide gene enhancer in B cells kinase beta antibody, anti-Inhibitorof nuclear factor kappa B kinase beta subunit antibody, anti-Inhibitor of nuclear factorkappa B kinase subunit beta antibody, anti-MGC131801 antibody, anti-NFKBIKBantibody, anti-Nuclear factor NF kappa B inhibitor kinase beta antibody, anti-Nuclearfactor of kappa light chain gene enhancer in B cells inhibitor antibody.
Gene Symbol: IKBKB
Gene ID 3551
NCBI Accession NP_001547
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Useful in Immunohistochemistry-Paraffin See Page et al.
Recommended dilutions: Flow Cytometry 0.25-0.5 µg/ 10^6 cells, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunoprecipitation 1:10-1:500, Western Blot 2-4 µg/mL
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.05 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
Western Blotting (WB) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Western blot analysis of 30 µg of total cell lysate from A) Daudi, B) HeLa, and C) mo...
Product cited in: Santos, Perez, Segrelles et al.: "Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis." in: The Journal of biological chemistry, Vol. 278, Issue 15, pp. 13422-30, 2003 (PubMed).

Ashikawa, Majumdar, Banerjee et al.: "Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 11, pp. 6490-7, 2002 (PubMed).

Anto, Mukhopadhyay, Shishodia et al.: "Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2." in: Carcinogenesis, Vol. 23, Issue 9, pp. 1511-8, 2002 (PubMed).

Background publications Majumdar, Aggarwal: "Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2911-20, 2001 (PubMed).

Li, Van Antwerp, Mercurio et al.: "Severe liver degeneration in mice lacking the IkappaB kinase 2 gene." in: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 321-5, 1999 (PubMed).

Delhase, Hayakawa, Chen et al.: "Positive and negative regulation of IkappaB kinase activity through IKKbeta subunit phosphorylation." in: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 309-13, 1999 (PubMed).

Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).

Verma, Stevenson: "IkappaB kinase: beginning, not the end." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 11758-60, 1997 (PubMed).

DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).

Malinin, Boldin, Kovalenko et al.: "MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1." in: Nature, Vol. 385, Issue 6616, pp. 540-4, 1997 (PubMed).

Lee, Hagler, Chen et al.: "Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway." in: Cell, Vol. 88, Issue 2, pp. 213-22, 1997 (PubMed).

Verma, Stevenson, Schwarz et al.: "Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation." in: Genes & development, Vol. 9, Issue 22, pp. 2723-35, 1995 (PubMed).

Catalog No. ABIN252473

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