You are viewing an incomplete version of our website. Please click to reload the website as full version.

IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta)

Details for Product anti-IKBKB Antibody No. ABIN252473, Supplier: Log in to see
Antigen
  • ikk-beta
  • ikk2
  • ikkb
  • ikkbeta
  • IKBKB
  • IKKB
  • CG4201
  • DIK
  • DLAK
  • DmIKK
  • DmIKKBeta
  • DmIKKb
  • DmIKKbeta
  • DmIkkbeta
  • Dmel\\CG4201
  • Dmikkb
  • Dmikkbeta
  • IK
  • IKK
  • IKK-beta
  • IKKb
  • IKKbeta
  • IRD-5
  • IRD5
  • Ik
  • Ikkb
  • Ikkbeta
  • Ird-5
  • Ird5
  • LRK
  • Lak
  • anon-89Bd
  • dIKK
  • dIKK-beta
  • dIKKbeta
  • dLak
  • dmIKKbeta
  • ikk
  • ird
  • ird5/DmIkk[beta]
  • IKK2
  • NFKBIKB
  • AIM-1
  • AI132552
  • IKK-2
  • IKK[b]
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • immune response deficient 5
  • inhibitor of kappaB kinase beta
  • ikbkb
  • IKBKB
  • ird5
  • Ikbkb
Alternatives
anti-Human IKBKB antibody for Western Blotting
Reactivity
Human, Mouse (Murine)
657
229
161
22
13
7
7
6
5
4
1
1
Host
Mouse
557
114
9
2
Clonality (Clone)
Monoclonal ()
Conjugate
This IKBKB antibody is un-conjugated
38
37
37
33
31
31
9
7
5
5
5
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
311
279
264
99
74
72
68
40
36
33
6
6
6
4
3
3
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
Clone 10AG2
Isotype IgG1
Specificity An approx. 87 kDa band should be observed.
Purification Protein G purified
Alternative Name IKK beta (IKBKB Antibody Abstract)
Background Gene Symbol: IKBKB
Molecular Weight Theoretical MW: 86.564 kDa
Gene ID 3551
UniProt O14920
Research Area Immunology, Innate Immunity, Atherosclerosis, Transcription Factors, Apoptosis/Necrosis
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades
Application Notes Western Blot 2 - 4 μg/mL, Simple Western 1:12.5, Flow Cytometry 0.25 - 0.5 μg/ 10^6 cells, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, CyTOF-readyUseful in Immunohistochemistry-Paraffin See Page et al. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24825920) In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Western blot analysis of 30 µg of total cell lysate from A) Daudi, B) HeLa, and C) mo...
Simple Western (SimWes) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Simple Western: IKK beta Antibody (10AG2) [ABIN2524739] - Simple Western lane view sh...
Flow Cytometry (FACS) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Flow Cytometry: IKK beta Antibody (10AG2) [ABIN2524739] - Analysis using the Alexa Fl...
Flow Cytometry (FACS) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Flow Cytometry: IKK beta Antibody (10AG2) [ABIN2524739] - Analysis of PE conjugate of...
Flow Cytometry (FACS) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Flow Cytometry: IKK beta Antibody (10AG2) [ABIN2524739] - Analysis using Alexa Fluor ...
Flow Cytometry (FACS) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Flow Cytometry: IKK beta Antibody (10AG2) [ABIN2524739] - Intracellular staining of H...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (ABIN252473) Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [ABIN2524739] - Formalin-fix...
Product cited in: Liu, Ibi, Taniguchi, Lee, Herrema, Akosman, Mucka, Salazar Hernandez, Uyar, Park, Karin, Ozcan: "Inflammation Improves Glucose Homeostasis through IKKβ-XBP1s Interaction." in: Cell, Vol. 167, Issue 4, pp. 1052-1066.e18, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Ren, Takahashi, Liu, Loughran, Sun, Wang, Cheng: "HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains." in: Oncogene, Vol. 34, Issue 3, pp. 334-45, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Gray, Remouchamps, McCorkell, Solt, Dejardin, Orange, May: "Noncanonical NF-κB signaling is limited by classical NF-κB activity." in: Science signaling, Vol. 7, Issue 311, pp. ra13, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Breuer, Becker, Brechmann, Mock, Arnold, Krammer: "The protein phosphatase 2A regulatory subunit B56γ mediates suppression of T cell receptor (TCR)-induced nuclear factor-κB (NF-κB) activity." in: The Journal of biological chemistry, Vol. 289, Issue 21, pp. 14996-5004, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Paul, Traver, Kashyap, Washington, Latoche, Schaefer: "T cell receptor signals to NF-κB are transmitted by a cytosolic p62-Bcl10-Malt1-IKK signalosome." in: Science signaling, Vol. 7, Issue 325, pp. ra45, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Buhrmann, Busch, Shayan, Shakibaei: "Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells." in: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22048-62, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Das, Manna, Sinha, Datta, Das, Chakraborty, Ghosh, Saha, Dey: "Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model." in: PLoS ONE, Vol. 9, Issue 5, pp. e97599, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Bimonte, Barbieri, Palma, Luciano, Rea, Arra: "Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer." in: BioMed research international, Vol. 2013, pp. 810423, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012 (PubMed).

Phromnoi, Reuter, Sung, Prasad, Kannappan, Yadav, Chanmahasathien, Limtrakul, Aggarwal et al.: "A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IκB kinase activation and sensitizes tumor cells to apoptosis by ..." in: Molecular pharmacology, Vol. 79, Issue 2, pp. 279-89, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Page, Cascallana, Casanova, Navarro, Alameda, Pérez, Bravo, Ramírez: "IKKβ overexpression leads to pathologic lesions in stratified epithelia and exocrine glands and to tumoral transformation of oral epithelia." in: Molecular cancer research : MCR, Vol. 9, Issue 10, pp. 1329-38, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Harikumar, Sung, Pandey, Guha, Krishnan, Aggarwal: "Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway." in: Molecular pharmacology, Vol. 77, Issue 5, pp. 818-27, 2010 (PubMed).

Huang, Ren, Guan, Jiang, Cheng: "HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB." in: The Journal of biological chemistry, Vol. 284, Issue 10, pp. 6208-17, 2009 (PubMed). (Sample species: Human). Further details: Western Blotting

Koch, Maeda, He, Karin, Leffert: "Targeted deletion of hepatocyte Ikkbeta confers growth advantages." in: Biochemical and biophysical research communications, Vol. 380, Issue 2, pp. 349-54, 2009 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Jin, Lee, Kim, Chung, Lee, Lee: "cIAP1, cIAP2, and XIAP act cooperatively via nonredundant pathways to regulate genotoxic stress-induced nuclear factor-kappaB activation." in: Cancer research, Vol. 69, Issue 5, pp. 1782-91, 2009 (PubMed). Further details: Immunoprecipitation

Kasinski, Du, Thomas, Zhao, Sun, Khuri, Wang, Shoji, Sun, Snyder, Liotta, Fu: "Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin." in: Molecular pharmacology, Vol. 74, Issue 3, pp. 654-61, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting

Pandey, Sung, Kunnumakkara, Sethi, Chaturvedi, Aggarwal: "Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis." in: Cancer research, Vol. 68, Issue 13, pp. 5370-9, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting

Kunnumakkara, Ichikawa, Anand, Mohankumar, Hema, Nair, Aggarwal et al.: "Coronarin D, a labdane diterpene, inhibits both constitutive and inducible nuclear factor-kappa B pathway activation, leading to potentiation of apoptosis, inhibition of invasion, and suppression of ..." in: Molecular cancer therapeutics, Vol. 7, Issue 10, pp. 3306-17, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting

Leis, Sanchis, Pérez: "Deletion of the N-terminus of IKKgamma induces apoptosis in keratinocytes and impairs the AKT/PTEN signaling pathway." in: Experimental cell research, Vol. 313, Issue 4, pp. 742-52, 2007 (PubMed). (Sample species: Mouse (Murine)).

Jackson-Bernitsas, Ichikawa, Takada, Myers, Lin, Darnay, Chaturvedi, Aggarwal: "Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma." in: Oncogene, Vol. 26, Issue 10, pp. 1385-97, 2007 (PubMed). Further details: Western Blotting