TLR4 antibody (Toll-Like Receptor 4) (AA 400-450)

Details for Product anti-TLR4 Antibody No. ABIN252535, Supplier: Log in to see
Antigen
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll-like receptor 4
  • TLR4
  • Tlr4
Alternatives
anti-Mouse (Murine) TLR4 antibody for Blocking Peptide
Epitope
AA 400-450, Internal Region
41
34
29
27
16
16
16
15
13
13
13
11
11
10
8
6
6
5
5
5
5
5
5
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
500
257
142
75
51
51
43
26
12
10
4
2
2
1
1
Host
Rabbit
291
252
60
9
Clonality
Polyclonal
Conjugate
This TLR4 antibody is un-conjugated
47
42
40
19
15
13
12
11
10
8
8
8
8
7
7
6
6
6
6
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
365
292
135
133
122
106
98
87
34
27
26
24
21
16
12
8
8
4
4
3
3
2
2
1
1
1
Options
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Immunogen A synthetic peptide made to an internal portion of the mouse TLR4 protein (between residues 400-450) [NP_612564]
Purification Immunogen affinity purified
Alternative Name TLR4 (TLR4 Antibody Abstract)
Background Gene Symbol: TLR4
Molecular Weight Theoretical MW: 75 kDa
Gene ID 7099
UniProt Q9QUK6
Research Area Adaptive Immunity, Signaling, Immunology, Innate Immunity, Atherosclerosis, Inflammation
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
Application Notes Western Blot 1-3 μg/mL, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:50, Immunocytochemistry/Immunofluorescence 1:25-1:75, Immunohistochemistry-Paraffin 1:50This TLR4 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Western Blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for TLR4 antibody
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry-Paraffin Embedded Sections Protocol Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.12 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-TLR4 antibody (Toll-Like Receptor 4) (AA 400-450) (ABIN252535) Western Blot: TLR4 Antibody [ABIN252535] - Western blot analysis of TLR4 using ABIN25...
Immunohistochemistry (IHC) image for anti-TLR4 antibody (Toll-Like Receptor 4) (AA 400-450) (ABIN252535) Immunohistochemistry: TLR4 Antibody [ABIN252535] - IHC analysis of TLR4 in formalin-f...
Immunohistochemistry (IHC) image for anti-TLR4 antibody (Toll-Like Receptor 4) (AA 400-450) (ABIN252535) Immunocytochemistry: TLR4 Antibody [ABIN252535] - ICC analysis of TLR4 in formalin-fi...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TLR4 antibody (Toll-Like Receptor 4) (AA 400-450) (ABIN252535) Immunohistochemistry-Paraffin: TLR4 Antibody [ABIN2525351] - Analysis of TLR4 in form...
Immunofluorescence (IF) image for anti-TLR4 antibody (Toll-Like Receptor 4) (AA 400-450) (ABIN252535) Immunocytochemistry/Immunofluorescence: TLR4 Antibody [ABIN2525351] - TLR4 antibody w...
Product cited in: Li, Kroin, Kc, Gibson, Chen, Corbett, Pahan, Fayyaz, Kim, van Wijnen, Suh, Kim, Im: "Altered spinal microRNA-146a and the microRNA-183 cluster contribute to osteoarthritic pain in knee joints." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 28, Issue 12, pp. 2512-22, 2013 (PubMed).

Lu, Li, Samuvel, Jin, Zhang, Lopes-Virella, Huang et al.: "MD-2 is involved in the stimulation of matrix metalloproteinase-1 expression by interferon-γ and high glucose in mononuclear cells - a potential role of MD-2 in Toll-like receptor 4-independent ..." in: Immunology, Vol. 140, Issue 3, pp. 301-13, 2013 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Flores-Espinosa, Pineda-Torres, Vega-Sánchez, Estrada-Gutiérrez, Espejel-Nuñez, Flores-Pliego, Maida-Claros, Paredes-Vivas, Morales-Méndez, Sosa-González, Chávez-Mendoza, Zaga-Clavellina: "Progesterone elicits an inhibitory effect upon LPS-induced innate immune response in pre-labor human amniotic epithelium." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 71, Issue 1, pp. 61-72, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Cammarota, Bertolini, Pennesi, Bucci, Gottardi, Garlanda, Laghi, Barberis, Sessa, Noonan, Albini: "The tumor microenvironment of colorectal cancer: stromal TLR-4 expression as a potential prognostic marker." in: Journal of translational medicine, Vol. 8, pp. 112, 2010 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Barajon, Serrao, Arnaboldi, Opizzi, Ripamonti, Balsari, Rumio: "Toll-like receptors 3, 4, and 7 are expressed in the enteric nervous system and dorsal root ganglia." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 11, pp. 1013-23, 2009 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry

El Shikh, El Sayed, Wu, Szakal, Tew: "TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 7, pp. 4444-50, 2007 (PubMed).

Herber, Maloney, Roth, Freeman, Morgan, Gordon: "Diverse microglial responses after intrahippocampal administration of lipopolysaccharide." in: Glia, Vol. 53, Issue 4, pp. 382-91, 2006 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Carrithers, Tandon, Canosa, Michaud, Graesser, Madri: "Enhanced susceptibility to endotoxic shock and impaired STAT3 signaling in CD31-deficient mice." in: The American journal of pathology, Vol. 166, Issue 1, pp. 185-96, 2005 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Flow Cytometry

Background publications Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).

Takeuchi, Hoshino, Kawai, Sanjo, Takada, Ogawa, Takeda, Akira: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).

Muzio, Natoli, Saccani, Levrero, Mantovani: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Medzhitov, Preston-Hurlburt, Janeway: "A human homologue of the Drosophila Toll protein signals activation of adaptive immunity." in: Nature, Vol. 388, Issue 6640, pp. 394-7, 1997 (PubMed).