Toll-Like Receptor 4 (TLR4) (AA 400-450) antibody

Details for Product No. ABIN252535
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms ARMD10, CD284, TLR-4, TOLL, Lps, Ly87, Ran/M1, Rasl2-8
Epitope
AA 400-450
(25), (22), (16), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(228), (70), (25), (20), (20), (16), (11), (9), (1), (1)
Host
Rabbit
(148), (121), (9), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(25), (23), (16), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(154), (149), (67), (57), (52), (51), (37), (14), (14), (13), (10), (7), (3), (2), (1)
Pubmed 6 references available
Quantity 0.1 mL
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN252535
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide made to an internal portion of the mouse TLR4 protein (between residues 400-450)
Isotype IgG
Purification affinity purified
Alternative Name CD284 / TLR4
Background The Toll-like receptor (TLR) family in mammal comprises a family of transmembraneproteins characterized by multiple copies of leucine rich repeats in the extracellulardomain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts inDrosophila, TLRs signal through adaptor molecules (1). The TLR family is aphylogenetically conserved mediator of innate immunity that is essential for microbialrecognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3).Among this family of receptors, TLR2 and TLR4 have been most studied. These studieshave suggested that TLR2 and TLR4 may serve as potential main mediators of LPSsignaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 aminoacids with approximate molecular weight of 90 kDa (6). Alternate Names: anti-TLR-4 antibody, anti-Toll-like Receptor 4 antibody.
Gene Symbol: TLR4
Gene ID 7099, 21898
Research Area Signaling, Immunology, Inflammation
Application Notes This TLR4 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Western Blot where a band is seen ~75 kDa.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:25-1:75, Immunohistochemistry 1:50, Immunohistochemistry-Paraffin 1:50, Western Blot 1-3 µg/mL
Protocol Western Blot protocol specific for TLR4 antibody
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry-Paraffin Embedded Sections Protocol Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.12 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-Toll-Like Receptor 4 (TLR4) (AA 400-450) antibody Western Blot: TLR4 Antibody [ABIN252535] - Western blot analysis of TLR4 using ABIN252535 at 2 ug/ml on partial recombinant mouse TLR4 protein.
anti-Toll-Like Receptor 4 (TLR4) (AA 400-450) antibody (2) Immunohistochemistry: TLR4 Antibody [ABIN252535] - IHC analysis of TLR4 in formalin-fixed, paraffin-embedded normal (a) and tumor (b) prostate sections using ABIN252535 at 1:50 dilution. Data courtesy of Dr. Regina Gandour-Edwards
anti-Toll-Like Receptor 4 (TLR4) (AA 400-450) antibody (3) Immunocytochemistry: TLR4 Antibody [ABIN252535] - ICC analysis of TLR4 in formalin-fixed Ramos (a) and 293 (b) cells using ABIN252535 at 1:10 dilution. Data courtesy of Dr. Regina Gandour-Edwards
General Medzhitov, Preston-Hurlburt, Janeway: "A human homologue of the Drosophila Toll protein signals activation of adaptive immunity." in: Nature, Vol. 388, Issue 6640, pp. 394-7, 1997 (PubMed).

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Takeuchi, Hoshino, Kawai et al.: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).

Carrithers, Tandon, Canosa et al.: "Enhanced susceptibility to endotoxic shock and impaired STAT3 signaling in CD31-deficient mice." in: The American journal of pathology, Vol. 166, Issue 1, pp. 185-96, 2005 (PubMed).

Validation Images
Did you look for something else?
back to top