Toll-Like Receptor 4 (TLR4) antibody

Antigen: Toll-Like Receptor 4 (TLR4)

Synonyms: TOLL, CD284, hToll, ARMD10, Lps, Ly87, Ran/M1, Rasl2-8

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN252535

Additional Information

Alternative name: CD284 / TLR4

Gene ID: 7099, 21898

Immunogen: This antibody was developed against a sythetic peptide corresponding to amino acids418-435 of mouse TLR4.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG

Description: The Toll-like receptor (TLR) family in mammal comprises a family of transmembraneproteins characterized by multiple copies of leucine rich repeats in the extracellulardomain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts inDrosophila, TLRs signal through adaptor molecules (1). The TLR family is aphylogenetically conserved mediator of innate immunity that is essential for microbialrecognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3).Among this family of receptors, TLR2 and TLR4 have been most studied. These studieshave suggested that TLR2 and TLR4 may serve as potential main mediators of LPSsignaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 aminoacids with approximate molecular weight of 90 kDa (6). Alternate Names: anti-TLR-4 antibody, anti-Toll-like Receptor 4 antibody.

Specificity: This antibody was tested against partial recombinant mouse TLR4 (extracellular portionplus His-tag), and a 75-80 kDa band was observed. ICC/IHC analysis has beenperformed by collaborator to validate usage in cell lines and human tissue samples. Atthis time data appears to be encouraging, but has yet to be duplicated and/or optimized. Testing is on-going, but researchers are encouraged to optimize. Species Reactivity: Cross reacts to Human and Mouse Not yet tested in other species.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 1/50,Western Blot 1-3 ug/m,Immunocytochemistry 1/10. Positive Controls: Recombinant protein

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin. Preservative: 0.05% Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Signaling, Immunology, Inflammation

Restrictions: For Research Use only

Images

Western Blot: TLR4 Antibody [ABIN252535] - Western blot analysis of TLR4 using ABIN252535 at 2 ug/ml on partial recombinant mouse TLR4 protein.

Immunohistochemistry: TLR4 Antibody [ABIN252535] - IHC analysis of TLR4 in formalin-fixed, paraffin-embedded normal (a) and tumor (b) prostate sections using ABIN252535 at 1:50 dilution. Data courtesy of Dr. Regina Gandour-Edwards

Immunocytochemistry: TLR4 Antibody [ABIN252535] - ICC analysis of TLR4 in formalin-fixed Ramos (a) and 293 (b) cells using ABIN252535 at 1:10 dilution. Data courtesy of Dr. Regina Gandour-Edwards

Publications

Medzhitov, Preston-Hurlburt, Janeway: "A human homologue of the Drosophila Toll protein signals activation of adaptive immunity." in: Nature, Vol. 388, Issue 6640, pp. 394-7, 1997 (PubMed).

Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Takeuchi, Hoshino, Kawai et al.: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, 2001 (PubMed).

Carrithers, Tandon, Canosa et al.: "Enhanced susceptibility to endotoxic shock and impaired STAT3 signaling in CD31-deficient mice." in: The American journal of pathology, Vol. 166, Issue 1, pp. 185-96, 2005 (PubMed).