Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) antibody

Details for Product No. ABIN252906, Supplier: Log in to see
Antigen
  • APPL
  • DIP13alpha
  • 2900057D21Rik
  • 7330406P05Rik
  • AI585782
  • AW209077
  • BB022931
  • C88264
  • DIP13
  • RGD1309388
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
  • APPL1
  • Appl1
Reactivity
Human
64
33
21
7
5
3
3
3
3
3
1
1
Host
Goat
44
19
3
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
60
32
17
12
3
3
1
1
1
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Immunogen Peptide with sequence C-NRYSRLSKKRENDK corresponding to internal region according to NP_036228.1.
Purification Immunogen affinity purified
Alternative Name APPL (APPL1 Antibody Abstract)
Background Gene Symbol: APPL1
Molecular Weight Theoretical MW: 80 kDa
Gene ID 26060
UniProt Q9UKG1
Research Area Apoptosis/Necrosis, Organogenesis
Application Notes Western Blot 0.3 - 1 μg/mL, Peptide ELISA Detection limit 1:64000WB: Approx. 80 kDa band observed in (lane 1) rat aortic smooth skeletal muscle cells, (lane 2) intensity increased after infection with human APPL1 adenovirus for 48h (calculated MW of 79.7 kDa band according to NP_036228.1). The blot was co-labeled with Tubulin-specific antibodies as a loading control. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) antibody (ABIN252906) Western Blot: APPL1 Antibody [ABIN252906] - APPL1 expression level after adenovirus t...
Western Blotting (WB) image for anti-Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) antibody (ABIN252906) Western Blot: APPL Antibody [ABIN2529061] - Staining of 1) Rat Aortic Smooth Muscle c...
Product cited in: Mao, Kikani, Riojas, Langlais, Wang, Ramos, Fang, Christ-Roberts, Hong, Kim, Liu, Dong: "APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function." in: Nature cell biology, Vol. 8, Issue 5, pp. 516-23, 2006 (PubMed).