BRCA1 antibody (Breast Cancer 1) (AA 1314-1864)

Details for Product anti-BRCA1 Antibody No. ABIN253014, Supplier: Log in to see
Antigen
  • BRCAI
  • BRCC1
  • BROVCA1
  • IRIS
  • PNCA4
  • PPP1R53
  • PSCP
  • RNF53
  • breast cancer 1
  • breast cancer 1, early onset
  • Brca1
  • BRCA1
Alternatives
anti-Human BRCA1 antibody for Biochemical Assay
Epitope
AA 1314-1864
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Reactivity
Human
558
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This BRCA1 antibody is un-conjugated
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Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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Options
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Immunogen Human BRCA1 corresponding to residues 1314-1864 [UniProt# P38398]
Clone RAY
Isotype IgG2a
Purification Protein G purified
Alternative Name BRCA1 (BRCA1 Antibody Abstract)
Background Gene Symbol: BRCA1
Molecular Weight Theoretical MW: 230 kDa
Gene ID 672
UniProt P38398
Research Area DNA/RNA, Proteolysis / Ubiquitin, Transcription Factors, Cancer
Pathways Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus
Application Notes Western Blot 2-4 μg/mL, Flow Cytometry 1 μg per million cells, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, CyTOF-readyThis BRCA1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot a band was observed ~220-240 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for BRCA1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS + 0.5 % BSA for 1 hour.
. Dilute the mouse anti-BRCA1 primary antibody in blocking buffer and incubate 2-2.5 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 2.76 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 1314-1864) (ABIN253014) Detection of BRCA1 in MCF-7 whole cell lysate using ABIN152030. 1 minute ECL detection.
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 1314-1864) (ABIN253014) Detection of BRCA1 using ABIN152030.
Flow Cytometry (FACS) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 1314-1864) (ABIN253014) Flow Cytometry: BRCA1 Antibody (RAY) [ABIN253014] - Intracellular flow cytometric sta...
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 1314-1864) (ABIN253014) Western Blot: BRCA1 Antibody (RAY) [ABIN253014] - Analysis of BRCA1 on MCF7 lysate.
Immunofluorescence (IF) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 1314-1864) (ABIN253014) Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (RAY) [ABIN253014] - BRCA (RAY...
Product cited in: Vanderperre, Lucier, Bissonnette, Motard, Tremblay, Vanderperre, Wisztorski, Salzet, Boisvert, Roucou: "Direct detection of alternative open reading frames translation products in human significantly expands the proteome." in: PLoS ONE, Vol. 8, Issue 8, pp. e70698, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Yin, Wei, Li, Cao, Guo: "Identification and molecular characterization of a new member of the peritrophic membrane proteins from the meadow moth, loxostege sticticalis." in: International journal of biological sciences, Vol. 6, Issue 5, pp. 491-8, 2010 (PubMed).

Kluk, Fu, Formolo, Zhang, Hindle, Man, Siegel, Berg, Deng, McCaffrey, Fu: "BP1, an isoform of DLX4 homeoprotein, negatively regulates BRCA1 in sporadic breast cancer." in: International journal of biological sciences, Vol. 6, Issue 5, pp. 513-24, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting

Okada, Ouchi: "Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization." in: The Journal of biological chemistry, Vol. 278, Issue 3, pp. 2015-20, 2003 (PubMed).