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BRCA1 antibody (Breast Cancer 1) (AA 1314-1864)

Details for Product anti-BRCA1 Antibody No. ABIN253015, Supplier: Log in to see
Antigen
  • BRCAI
  • BRCC1
  • BROVCA1
  • IRIS
  • PNCA4
  • PPP1R53
  • PSCP
  • RNF53
Alternatives
anti-Human BRCA1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 1314-1864
69
34
30
26
24
21
20
16
15
15
15
15
13
11
10
7
6
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
484
44
36
4
1
Host
Mouse
368
120
4
3
Clonality (Clone)
Monoclonal ()
Conjugate
This BRCA1 antibody is un-conjugated
18
17
15
9
9
6
6
6
6
6
6
6
6
6
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
313
127
119
106
105
71
69
54
8
7
4
4
2
1
1
1
Supplier
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Immunogen Human BRCA1 corresponding to residues 1314-1864 [UniProt# P38398]
Clone KEN
Isotype IgG3 kappa
Purification Protein A purified
Alternative Name BRCA1 (BRCA1 Antibody Abstract)
Background Gene Symbol: BRCA1
Molecular Weight Theoretical MW: 240 kDa
UniProt P38398
Research Area DNA/RNA, Proteolysis / Ubiquitin, Transcription Factors, Cancer
Pathways Cell Division Cycle, DNA Damage Repair
Application Notes Western Blot 2-4 μg/mL, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for BRCA1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS + 0.5 % BSA for 1 hour.
. Dilute the mouse anti-BRCA1 primary antibody in blocking buffer and incubate 2-2.5 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1.67 mg/mL
Buffer Tris-glycine, 150 mM NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 1314-1864) (ABIN253015) Detection of BRCA1 in U2OS whole cell lysate using ABIN152031. 1 minute ECL detection.
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 1314-1864) (ABIN253015) Detection of BRCA1 using ABIN152031.
Product cited in: Okada, Ouchi: "Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization." in: The Journal of biological chemistry, Vol. 278, Issue 3, pp. 2015-20, 2003 (PubMed).