Breast Cancer 1 (BRCA1) (AA 1314-1864) antibody

Details for Product No. ABIN253016
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Antigen
Synonyms BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PPP1R53, PSCP, RNF53
Epitope
AA 1314-1864
(44), (39), (24), (22), (17), (17), (15), (14), (14), (14), (12), (9), (7), (7), (7), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(378), (45), (25), (13), (1), (1), (1), (1)
Host
Mouse
(336), (60), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(17), (15), (12), (9), (9), (9), (9), (9), (9), (9), (9), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(230), (100), (90), (90), (74), (74), (58), (15), (9), (3), (2), (1)
Pubmed 1 reference available
Quantity 0.025 mL
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Catalog No. ABIN253016
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Immunogen Human BRCA1 corresponding to residues 1314-1864
Clone MU
Isotype IgG2b kappa
Purification Protein G purified
Alternative Name BRCA1 / RNF53
Background BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger proteincontaining a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms.The full length protein has a reported molecular weight of 208 kD. BRCA1 localizes tothe mitotic spindle microtubules, centriole walls, pericentriolar fibers at centrosomes.Unphosphorylated BRCA1 localizes on chromosomes from metaphase throughtelophase, phosphorylated BRCA1 resides in inner chromosomal structure, centrosome,cleavage furrow during prophase through telophase, and relocalizes to the perinuclearregion when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as atumor suppressor and can function as a secreted growth inhibitory protein, participate intranscription coupled repair of oxidative DNA damage, X-chromosome inactivation, andcan function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated byEts-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination andphosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has beenreported to interact with RNA polymerase II holoenzyme and BARD1. BRCA1 containsat least two nuclear localization signals and is proposed to be a tumor suppressorprotein. It is a serine phosphoprotein that undergoes hyperphosphorylation during lateG1 and S phases of the cell cycle and is transiently dephosphorylated early after Mphase. BRCA1 protein alters in a qualitative and quantitative manner during cell cycleprogression. The amount of BRCA1 protein is highest during S phase and remainselevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA1function confers an increased susceptibility for both breast and ovarian cancer. Alternate Names: anti-Breast Cancer 1 antibody, anti-Breast cancer 1 early onset antibody, anti-Breastcancer type 1 susceptibility protein antibody, anti-Breast Ovarian Cancer Susceptibilityantibody, anti-Papillary serous carcinoma of the peritoneum antibody, anti-PSCPantibody, anti-RNF53 antibody.
Gene Symbol: BRCA1
Gene ID 672
UniProt P38398
Application Notes In Western blot a band was observed ~220-240 kDa.
Recommended dilutions: Flow Cytometry 1 µg per million cells, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Western Blot 2-4 µg/mL
Protocol Protocol specific for BRCA1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS + 0.5 % BSA for 1 hour.
. Dilute the mouse anti-BRCA1 primary antibody in blocking buffer and incubate 2-2.5 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1.9 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Breast Cancer 1 (BRCA1) (AA 1314-1864) antibody Detection of BRCA1 using ABIN152032.
General Okada, Ouchi: "Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization." in: The Journal of biological chemistry, Vol. 278, Issue 3, pp. 2015-20, 2003 (PubMed).

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