Neutrophil Cytosolic Factor 4, 40kDa (NCF4) antibody

Details for Product No. ABIN253305, Supplier: Log in to see
Antigen
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
Reactivity
Human
117
58
44
7
7
5
4
4
3
3
Host
Goat
91
19
11
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
74
40
32
26
22
5
4
3
3
2
2
1
Options
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Immunogen Peptide with sequence C-KDFPEEDDPTN corresponding to internal region according to NP_000622.2, NP_038202.1.
Specificity This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Purification Immunogen affinity purified
Alternative Name NCF4 (NCF4 Antibody Abstract)
Background Gene Symbol: NCF4
Gene ID 4689
NCBI Accession NP_000622
Research Area Metabolism
Application Notes Western Blot 0.3 - 1 μg/mL, Immunohistochemistry 2 - 4 μg/mL, Immunohistochemistry-Paraffin 2 - 4 μg/mL, Peptide ELISA Detection limit 1:32000WB: Approx. 38 kDa band observed in Daudi lysates (calculated MW of 39.0 kDa band according to NP_000622.2 and 38.8 kDa band according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human tonsil shows staining of the cytoplasm in some cells.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) antibody (ABIN253305) ABIN253305 (0.5ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Pr...
Western Blotting (WB) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) antibody (ABIN253305) Western Blot: NCF4 Antibody [ABIN2533056] - Analysis of NCF4 antibody using ABIN25330...
Western Blotting (WB) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) antibody (ABIN253305) Western Blot: NCF4 Antibody [ABIN2533056] - HEK293 overexpressing P40PHOX and probed ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) antibody (ABIN253305) Immunohistochemistry-Paraffin: NCF4 Antibody [ABIN2533056] - (2.5ug/ml) staining of p...
Product cited in: Rioux, Xavier, Taylor, Silverberg, Goyette, Huett, Green, Kuballa, Barmada, Datta, Shugart, Griffiths, Targan, Ippoliti, Bernard, Mei, Nicolae, Regueiro, Schumm, Steinhart, Rotter, Duerr, Cho, Daly et al.: "Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. ..." in: Nature genetics, Vol. 39, Issue 5, pp. 596-604, 2007 (PubMed).