DYKDDDDK Epitope Tag antibody

Antigen: DYKDDDDK Epitope Tag

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN254301

Additional Information

Immunogen: Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptideDYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at theC-terminal end.

Isotype: IgG

Description: This is a useful tool for the localisation and characterisation of DDDDK tagged proteins. Alternate Names: Anti-DDDDK epitope tag antibody, Anti-DYKDDDDK epitope tag antibody, Anti-ECSepitope tag antibody, Anti-ECS tag antibody, Anti-Enterokinase Cleavage Site epitopetag antibody, Anti-Enterokinase Cleavage Site tag antibody, Anti-FLAG antibody.

Specificity: ABIN254301 is specific for the hydrophilic epitope tagging octapeptide sequenceDYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- orcarboxyl- terminus of target proteins.ABIN254301 has been tested against both the immunogen and recombinant proteinscontaining the DYKDDDDK sequence in ELISA and Western blotting and shows greaterbinding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequencethan the clones M1, M2 and M5, in the majority of assays.In Western blotting of bacterial extracts this antibody has been shown not to cross-reactwith any endogenous proteins. Species Reactivity: Cross reacts with synthetic peptide.

Application Details

Concentration: 0.5mg/ml

Purification: Aff - Purified

Buffer: 0.02M Potassium Phosphate and 0.15M Sodium Chloride. Preservative: Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Research Area: Tags/Labels

Restrictions: For Research Use only

Publications

Slootstra, Kuperus, Plueckthun et al.: "Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5." in: Molecular diversity, Vol. 2, Issue 3, pp. 156-64, 1997 (PubMed).

Lee, Qian, Rey et al.: "Anthracycline chemotherapy inhibits HIF-1 transcriptional activity and tumor-induced mobilization of circulating angiogenic cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 7, pp. 2353-8, 2009 (PubMed).