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DYKDDDDK Tag (C-Term) antibody

Details for Product No. ABIN254301, Supplier: Log in to see
Antigen
Epitope
C-Term
15
9
6
1
Reactivity
All Species
251
98
36
18
18
7
5
4
3
2
2
1
1
1
Host
Rabbit
179
111
34
31
17
Clonality
Polyclonal
Conjugate
Un-conjugated
39
21
18
8
8
8
8
6
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
299
141
127
99
85
59
39
10
9
9
7
6
4
4
4
4
3
3
3
3
2
1
1
1
1
1
1
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Immunogen Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Sequence DYKDDDDK
Specificity This antibody is specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. This antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays. In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.
Purification IgG purified
Alternative Name DYKDDDDK Epitope Tag
Research Area Tags/Labels
Application Notes Western Blot 1:2000-1:10000, ELISA 1:9000-1:450000In Western blotting of bacterial extracts, this antibody has been shown not to cross-react with any endogenous proteins.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer No buffer
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Product cited in: Heir, Srikumar, Bikopoulos, Bunda, Poon, Lee, Raught, Ohh: "Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling." in: The Journal of biological chemistry, Vol. 291, Issue 14, pp. 7357-72, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Heir, Sufan, Greer, Poon, Lee, Ohh: "DCNL1 functions as a substrate sensor and activator of cullin 2-RING ligase." in: Molecular and cellular biology, Vol. 33, Issue 8, pp. 1621-31, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Lee, Qian, Rey, Wei, Liu, Semenza: "Anthracycline chemotherapy inhibits HIF-1 transcriptional activity and tumor-induced mobilization of circulating angiogenic cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 7, pp. 2353-8, 2009 (PubMed).

Slootstra, Kuperus, Plückthun, Meloen: "Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5." in: Molecular diversity, Vol. 2, Issue 3, pp. 156-64, 1997 (PubMed).