His Tag antibody

Details for Product No. ABIN255788, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
24
22
14
3
2
Host
Mouse
434
184
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
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4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
554
385
166
107
98
76
69
25
13
11
9
7
7
6
6
5
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen PAX6 transcription factor linked to histidine tag
Clone AD1-1-10
Isotype IgG1
Purification Protein G purified
Alternative Name 6xHistidine Epitope Tag (HHHHHH)
Target Type Tag
Research Area Tags/Labels
Application Notes Western Blot 1:1000, Simple Western 1:250, Flow Cytometry 1:10-1:50, ELISA 1:100-1:500, Immunohistochemistry 1:100-1:300, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:100-1:300This 6-Histidine Epitope Tag Antibody (AD1.1.10) is useful for Western blot, Immunohistochemistry on frozen sections, ELISA, Flow Cytometry and Immunoprecipitation. This antibody is routinely tested in Western blot using Histidine-tagged recombinant proteins. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN255788) anti-His Tag antibody
Simple Western (SimWes) image for anti-His Tag antibody (ABIN255788) Simple Western: 6-Histidine Epitope Tag Antibody (AD1.1.10) [ABIN2557888] - Simple We...
Western Blotting (WB) image for anti-His Tag antibody (ABIN255788) Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [ABIN2557888] - Analysis us...
Western Blotting (WB) image for anti-His Tag antibody (ABIN255788) Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [ABIN2557888] - Analysis us...
Flow Cytometry (FACS) image for anti-His Tag antibody (ABIN255788) Flow Cytometry: 6-Histidine Epitope Tag Antibody (AD1.1.10) [ABIN2557888] - Analysis ...
Flow Cytometry (FACS) image for anti-His Tag antibody (ABIN255788) Flow Cytometry: 6-Histidine Epitope Tag Antibody (AD1.1.10) [ABIN2557888] - Analysis ...
Flow Cytometry (FACS) image for anti-His Tag antibody (ABIN255788) Flow Cytometry: 6-Histidine Epitope Tag Antibody (AD1.1.10) [ABIN2557888] - Analysis ...
Western Blotting (WB) image for anti-His Tag antibody (ABIN255788) Western Blot: His Tag Antibody (AD1.1.10) - Drosophila, 20 ug whole cell lysate
Product cited in: Tseng, Liu, Chung, Lin, Chen, Wang, Chen, Tsai, Cheng, Pan, Hu, Lee, Liao: "A fusion protein composed of receptor binding domain of vascular endothelial growth factor-A and constant region fragment of antibody: angiogenesis antagonistic activity." in: Cytotechnology, Vol. 63, Issue 3, pp. 285-93, 2011 (PubMed). Further details: ELISA

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