COL2A1 antibody (AA 231-330)

Catalog No. ABIN2559415

Product Details anti-COL2A1 Antibody

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Binding Specificity: AA 231-330

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Collagen alpha-1(II) chain

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-COL2A1 antibody (ABIN2559415)

Yang, Zhao, Wang, Liu, Ma, Huang, Liu: "Isolation and biological characterization of tendon-derived stem cells from fetal bovine." in: In vitro cellular & developmental biology. Animal, Vol. 52, Issue 8, pp. 846-56, (2017) (PubMed).

Shi, Ma, Zhang, Li, Jiang, Qin: "Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes." in: Integrative biology : quantitative biosciences from nano to macro, (2015) (PubMed).

Ren, Liu, Ma, Liu, Diao, Yang, Zhang, Xi, Hu: "Treatment of rabbit intervertebral disc degeneration with co-transfection by adeno-associated virus-mediated SOX9 and osteogenic protein-1 double genes in vivo." in: International journal of molecular medicine, Vol. 32, Issue 5, pp. 1063-8, (2014) (PubMed).

Xu, Liu, Liu, Lim, Chen, Lin: "Treatment with SiMiaoFang, an anti-arthritis chinese herbal formula, inhibits cartilage matrix degradation in osteoarthritis rat model." in: Rejuvenation research, Vol. 16, Issue 5, pp. 364-76, (2014) (PubMed).

Target Details for COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Alternative Name: Collagen alpha-1(II) chain (COL2A1 Products)

Background:

Synonyms: AOM, ANFH, SEDC, STL1, COL11A3, Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1

Background: Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Gene ID: 1280

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding