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Sphingosine Kinase 1 (SPHK1) (C-Term) antibody

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Synonyms CG1747, Dmel\\CG1747, SK1, Sphk1, SPHK1, F18E5.160, F18E5_160, sphingosine kinase 1, SPHK, 1110006G24Rik, Sk1, Spk1
(46), (25), (11), (10), (9), (7), (6), (6), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Mouse (Murine)
(162), (43), (37), (5), (4), (3), (2), (1), (1), (1), (1), (1)
(148), (17), (10)
(7), (6), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), ELISA
(122), (58), (35), (30), (22), (11), (10), (10), (9), (7), (2), (2), (1)
Pubmed 2 references available
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Quantity 0.1 mg
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Immunogen Synthetic peptide with sequence SWKPQQMPPPEEPL, representing the C-Terminus of the human protein according to NP_068807.
Isotype IgG
Specificity This antibody is expected to recognize all reported isoforms (NP_068807.2, NP_892010.2, NP_001136073.1). The variants NP_001136073.1 and NP_001136074.1 represent identical protein.
Purification affinity purified
Background SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate(SPP), a lipid mediator which has both intra- and extracellular functions. Intracellularly,SPP regulates proliferation and survival. Extracellularly it functions as a ligand forEDG1. SPHK1 also acts on D-erythro-sphingosine and to a lesser extent sphinganine,but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine,diacylglycerol, ceramide, or phosphatidylinositol. Competitive inhibitors of SPHK blockformation of SPP and selectively inhibit cellular proliferation induced by a variety offactors, including platelet-derived growth factor and serum. SPHK1 has also been foundto mediate TRAF2 activation (Xia et al 2002). TRAF2 is a member of the TNF receptorassociated family and is required for TNF-alpha activation of MAPK8/JNK and NFkB.SPHK1 is widely expressed, with highest levels in adult liver, kidney, heart and skeletalmuscle. Alternate Names: anti-Sphingosine Kinase 1 antibody, anti-SPHK antibody.
Gene Symbol: SPHK1
Gene ID 8877, 20698
NCBI Accession NP_068807
Application Notes Recommended dilutions: Peptide ELISA detection limit 1:16000, Western Blot 1:1000
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline containing [pH 7.3] with 0.5 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Sphingosine Kinase 1 (SPHK1) (C-Term) antibody (ABIN257695) Western Blot: SPHK1 Antibody [ABIN257695] - ABIN257695 staining (1ug/ml) of Human Col...
Product cited in: Igarashi, Okada, Hayashi et al.: "Sphingosine kinase 2 is a nuclear protein and inhibits DNA synthesis." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46832-9, 2003 (PubMed).

Background publications Kohama, Olivera, Edsall et al.: "Molecular cloning and functional characterization of murine sphingosine kinase." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 23722-8, 1998 (PubMed).

Catalog No. ABIN257695
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