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Sphingosine Kinase 1 (SPHK1) antibody

Antigen

Sphingosine Kinase 1 (SPHK1)
Synonyms SPHK, Sk1, Spk1, 1110006G24Rik, SK1, Sphk1, DmelCG1747, CG1747, SPHK1, F18E5.160, F18E5_160, SPHINGOSINE KINASE 1
Clonality Polyclonal
Host
Reactivity
Alternatives

Mouse (Murine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA
2 references available
Catalog no. ABIN257695
Quantity 0.1 mg  (0.5 mg/ml)  (Variants)
Price Product not available in this region.
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anti-Sphingosine Kinase 1 (SPHK1) antibody

Additional Information
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Gene ID 8877, 20698
NCBI Acession NP_068807
Immunogen Synthetic peptide with sequence SWKPQQMPPPEEPL, representing the C-Terminus ofthe human protein according to NP_068807. Reference Sequence: NP_068807.
Cross-Reactivity Mouse (Murine)
Isotype IgG
Description SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate(SPP), a lipid mediator which has both intra- and extracellular functions. Intracellularly,SPP regulates proliferation and survival. Extracellularly it functions as a ligand forEDG1. SPHK1 also acts on D-erythro-sphingosine and to a lesser extent sphinganine,but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine,diacylglycerol, ceramide, or phosphatidylinositol. Competitive inhibitors of SPHK blockformation of SPP and selectively inhibit cellular proliferation induced by a variety offactors, including platelet-derived growth factor and serum. SPHK1 has also been foundto mediate TRAF2 activation (Xia et al 2002). TRAF2 is a member of the TNF receptorassociated family and is required for TNF-alpha activation of MAPK8/JNK and NFkB.SPHK1 is widely expressed, with highest levels in adult liver, kidney, heart and skeletalmuscle. Alternate Names: anti-Sphingosine Kinase 1 antibody, anti-SPHK antibody.
Specificity Species Reactivity: Cross-reacts with Human. Not yet tested in other species.

Application Details
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Application Notes This antibody can be used in Western Blot. Recommended Starting Dilutions*:WB: 1-3 ug/ml
Concentration 0.5 mg/ml
Purification Aff - Purified
Buffer Tris saline containing [pH7.3] with 0.5% BSA. Preservative: 0.02% Na azide.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Restrictions For Research Use only

Publications
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Kohama, Olivera, Edsall et al.: "Molecular cloning and functional characterization of murine sphingosine kinase." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 23722-8, 1998 (PubMed).

Igarashi, Okada, Hayashi et al.: "Sphingosine kinase 2 is a nuclear protein and inhibits DNA synthesis." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46832-9, 2003 (PubMed).

Alternatives
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Alternatives for antigen "Sphingosine Kinase 1 (SPHK1)", type "Antibodies"
Hosts Rabbit (66), Goat (10)
Reactivities Human (56), Mouse (Murine) (29), Rat (Rattus) (29), Cow (Bovine) (21), Dog (Canine) (18), Primate (1)
Applications Western Blotting (WB) (42), ELISA (24), Immunofluorescence (IF) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (IHC) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Paraffin-embedded Sections) pro (IHC (p)+) (1)
Conjugates Biotin (2), FITC (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes N-Term (12), C-Term (5), Internal Region (3), Center (2), Long Isoform (2), pSer225 (2), AA 150-200 (1), AA 18-29 (1), C-Term,Arg414 (1), Long Form (1), Middle Region (1), N-Term,Pro74 (1)