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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (HRP)

Details for Product anti-GAPDH Antibody No. ABIN258130, Supplier: Log in to see
Antigen
  • CG8893
  • Dmel\\CG8893
  • GA3PDH
  • GADPH
  • GAP
  • GAPDH
  • GAPDH II
  • GAPDH2
  • Gapd
  • Gapdh
  • Gapdh-2
  • Gapdh13F
  • GAPD
  • g3pd
  • gapd
  • GB14798
  • GAPC-2
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • T6J4.17
  • T6J4_17
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • BEST:GH12586
  • CG12055
  • Dmel\\CG12055
  • GAPDH I
  • GAPDH-1
  • GAPDH1
  • GAPDHI
  • Gapdh-1
  • Gapdh43E
  • gadph
  • gapdh
  • gapdh-1
  • gh12586
  • KNC-NDS6
  • G3PD
  • cb609
  • mg:bb02e05
  • wu:fb33a10
  • wu:ft80f05
  • G3PDH
Reactivity
Bird (Avian), Cow (Bovine), Dog (Canine), Chicken, Human, Mammalian, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Mustelid
543
220
193
111
56
51
44
31
29
25
18
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13
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8
8
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4
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3
3
2
2
1
1
1
1
1
1
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1
Host
Mouse
335
229
58
31
20
Clonality (Clone)
Monoclonal ()
Conjugate
This GAPDH antibody is conjugated to HRP
42
39
18
6
6
6
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4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
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1
Application
Western Blotting (WB)
587
275
147
127
86
55
44
26
21
17
10
9
7
6
5
5
4
4
2
2
2
2
1
1
1
1
Supplier
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Immunogen rabbit GAPDH
Clone 71-1
Isotype IgM
Specificity GAPDH (71.1)
Cross-Reactivity Turkey, Monkey, Dog (Canine), Chicken, Human, Cow (Bovine), Rat (Rattus), Mouse (Murine), Mink, Rabbit, Hamster
No Cross-Reactivity Prokaryotes
Purification affinity purified
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background The enzyme glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) is a tetramer ofidentical chains that catalyzes the reversible oxidative phosphorylation ofglyceraldehyde-phosphate in the presence of inorganic phosphate and nicotinamideadenine dinucleotide (NAD). This is an important energy-yielding step in carbohydratemetabolism. GAPDH is found in almost all species with a low rate of evolutionarychanges.1 GAPDH was found also to bind to several proteins such as: actin, tubulin,amyloid precursor, polyglutamine peptides, DRPLA, and huntingtin. In human embryonickidney and mouse neuroblastoma cell lines, it was shown that nuclear translocation andassociated neurotoxicity of mutant huntingtin is mediated by a ternary complex ofhuntingtin, GAPDH, and a ubiquitin E3 ligase named SIAH1. Over-expression ofGAPDH or SIAH1 enhances nuclear translocation of mutant huntingtin and cytotoxicity.2GAPDH was also found to be part of the multicomponent OCT1 coactivator complex,OCA-S. This complex is essential for the S phase-dependent histone H2B transcription.This association links the H2B transcriptional machinery to cell cycle regulation andpossibly to the cellular metabolic state (redox status).3. Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.
Gene Symbol: GAPDH
Gene ID 2597
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Pathways
Application Notes Recommended dilutions: Western Blot 1:35000-1:70000
Restrictions For Research Use only
Format Liquid
Buffer 0.01 PBS, pH 7.4, Thimerosal
Preservative Thimerosal (Merthiolate)
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase)  (HRP) (ABIN258130) anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (HRP)
Product cited in: Bae, Hara, Cascio et al.: "Mutant huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 9, pp. 3405-9, 2006 (PubMed).

Zheng, Roeder, Luo: "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component." in: Cell, Vol. 114, Issue 2, pp. 255-66, 2003 (PubMed).