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Details for Product No. ABIN258130

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (HRP)

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Antigen
Synonyms
G3PD, GAPD, Gapd, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, G3PDH, CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh, Gapdh-2, Gapdh13F, BEST:GH12586, CG12055,  ... show more
Reactivity
»Alternatives Bird (Avian), Cow (Bovine), Dog (Canine), Chicken, Human, Mammalian, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Mustelid
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
Application
»Alternatives Western Blotting (WB)
Pubmed 2 references available
Catalog no. ABIN258130
Quantity 50 µL
Price
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Immunogen rabbit GAPDH.
Clone 71-1
Isotype IgM
Specificity Species Reactivity: Also cross-reacts with Mink and Turkey. Does not cross-react with non-vertebrate andprokaryote GAPDH.
Cross-Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
Alternative Name GAPDH
Background The enzyme glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) is a tetramer ofidentical chains that catalyzes the reversible oxidative phosphorylation ofglyceraldehyde-phosphate in the presence of inorganic phosphate and nicotinamideadenine dinucleotide (NAD). This is an important energy-yielding step in carbohydratemetabolism. GAPDH is found in almost all species with a low rate of evolutionarychanges.1 GAPDH was found also to bind to several proteins such as: actin, tubulin,amyloid precursor, polyglutamine peptides, DRPLA, and huntingtin. In human embryonickidney and mouse neuroblastoma cell lines, it was shown that nuclear translocation andassociated neurotoxicity of mutant huntingtin is mediated by a ternary complex ofhuntingtin, GAPDH, and a ubiquitin E3 ligase named SIAH1. Over-expression ofGAPDH or SIAH1 enhances nuclear translocation of mutant huntingtin and cytotoxicity.2GAPDH was also found to be part of the multicomponent OCT1 coactivator complex,OCA-S. This complex is essential for the S phase-dependent histone H2B transcription.This association links the H2B transcriptional machinery to cell cycle regulation andpossibly to the cellular metabolic state (redox status).3. Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.
Gene ID 2597
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes Suggested working dilutions: Western Blot 1/35000-1/70000
Restrictions For Research Use only
Format Liquid
Concentration 2.7 mg/ml
Buffer 0.01 PBS, pH 7.4. Preservative: 0.01% thimerosal.
Preservative Thimerosal (Merthiolate)
Storage 4 °C
Zheng, Roeder, Luo: "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component." in: Cell, Vol. 114, Issue 2, pp. 255-66, 2003 (PubMed).

Bae, Hara, Cascio et al.: "Mutant huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 9, pp. 3405-9, 2006 (PubMed).

Alternatives for antigen "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)", type "Antibodies"
Hosts (250), (199), (37), (22), (20)
Reactivities (398), (244), (211), (107), (59), (38), (37), (35), (27), (25), (24), (20), (16), (14), (12), (11), (9), (9), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Applications (446), (238), (140), (81), (79), (60), (50), (37), (17), (13), (7), (6), (5), (5), (4), (2), (2), (1), (1)
Conjugates (30), (23), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1)
Epitopes (82), (34), (11), (10), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
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