Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (HRP)

Details for Product No. ABIN258130
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Antigen
Synonyms
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, gl ... show more
CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapd, Gapdh, Gapdh-2, Gapdh13F, GAPD, g3pd, gapd, GB14798, GAPC-2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, T6J4.17, T6J4_17, glyceraldehyde-3-phosphate dehydrogenase C2, BEST:GH12586, CG12055, Dmel\\CG12055, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, KNC-NDS6, G3PD, cb609, mg:bb02e05, wu:fb33a10, wu:ft80f05, G3PDH show less
Reactivity
Bird (Avian), Cow (Bovine), Dog (Canine), Chicken, Human, Mammalian, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Mustelid
(401), (199), (168), (90), (42), (32), (29), (26), (18), (18), (17), (14), (12), (10), (9), (9), (8), (8), (7), (6), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(271), (193), (34), (18), (16)
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
(31), (28), (11), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(468), (211), (135), (83), (75), (65), (31), (30), (29), (19), (15), (10), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 0.05 mL
Shipping to United States (Change)
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Catalog No. ABIN258130
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Immunogen rabbit GAPDH
Clone 71-1
Isotype IgM
Specificity GAPDH (71.1)
Cross-Reactivity Turkey, Monkey, Dog (Canine), Chicken, Human, Cow (Bovine), Rat (Rattus), Mouse (Murine), Mink, Rabbit, Hamster
No Cross-Reactivity Prokaryotes
Purification affinity purified
Alternative Name GAPDH
Background The enzyme glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) is a tetramer ofidentical chains that catalyzes the reversible oxidative phosphorylation ofglyceraldehyde-phosphate in the presence of inorganic phosphate and nicotinamideadenine dinucleotide (NAD). This is an important energy-yielding step in carbohydratemetabolism. GAPDH is found in almost all species with a low rate of evolutionarychanges.1 GAPDH was found also to bind to several proteins such as: actin, tubulin,amyloid precursor, polyglutamine peptides, DRPLA, and huntingtin. In human embryonickidney and mouse neuroblastoma cell lines, it was shown that nuclear translocation andassociated neurotoxicity of mutant huntingtin is mediated by a ternary complex ofhuntingtin, GAPDH, and a ubiquitin E3 ligase named SIAH1. Over-expression ofGAPDH or SIAH1 enhances nuclear translocation of mutant huntingtin and cytotoxicity.2GAPDH was also found to be part of the multicomponent OCT1 coactivator complex,OCA-S. This complex is essential for the S phase-dependent histone H2B transcription.This association links the H2B transcriptional machinery to cell cycle regulation andpossibly to the cellular metabolic state (redox status).3. Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.
Gene Symbol: GAPDH
Gene ID 2597
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes Recommended dilutions: Western Blot 1:35000-1:70000
Restrictions For Research Use only
Format Liquid
Buffer 0.01 PBS, pH 7.4, Thimerosal
Preservative Thimerosal (Merthiolate)
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (HRP) anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (HRP)
General Zheng, Roeder, Luo: "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component." in: Cell, Vol. 114, Issue 2, pp. 255-66, 2003 (PubMed).

Bae, Hara, Cascio et al.: "Mutant huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 9, pp. 3405-9, 2006 (PubMed).

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