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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (HRP)

Antigen

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Synonyms
G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, G ... show more
Clonality Monoclonal (71-1)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Application
Alternatives Western Blotting (WB)
2 references available
Catalog no. ABIN258130
Quantity 50 µl  (2.7 mg/ml)
Price Product not available in this region.
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (HRP)

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name GAPDH
Gene ID 2597
Immunogen rabbit GAPDH.
Cross-Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
Isotype IgM
Clone 71-1
Description The enzyme glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) is a tetramer ofidentical chains that catalyzes the reversible oxidative phosphorylation ofglyceraldehyde-phosphate in the presence of inorganic phosphate and nicotinamideadenine dinucleotide (NAD). This is an important energy-yielding step in carbohydratemetabolism. GAPDH is found in almost all species with a low rate of evolutionarychanges.1 GAPDH was found also to bind to several proteins such as: actin, tubulin,amyloid precursor, polyglutamine peptides, DRPLA, and huntingtin. In human embryonickidney and mouse neuroblastoma cell lines, it was shown that nuclear translocation andassociated neurotoxicity of mutant huntingtin is mediated by a ternary complex ofhuntingtin, GAPDH, and a ubiquitin E3 ligase named SIAH1. Over-expression ofGAPDH or SIAH1 enhances nuclear translocation of mutant huntingtin and cytotoxicity.2GAPDH was also found to be part of the multicomponent OCT1 coactivator complex,OCA-S. This complex is essential for the S phase-dependent histone H2B transcription.This association links the H2B transcriptional machinery to cell cycle regulation andpossibly to the cellular metabolic state (redox status).3. Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.
Specificity Species Reactivity: Also cross-reacts with Mink and Turkey. Does not cross-react with non-vertebrate andprokaryote GAPDH.
Synonyms G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Application Details
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Application Notes Suggested working dilutions: Western Blot 1/35000-1/70000
Concentration 2.7 mg/ml
Buffer 0.01 PBS, pH 7.4. Preservative: 0.01% thimerosal.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Restrictions For Research Use only

Publications
Application Details Images Alternatives back to top

Zheng, Roeder, Luo: "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component." in: Cell, Vol. 114, Issue 2, pp. 255-66, 2003 (PubMed).

Bae, Hara, Cascio et al.: "Mutant huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 9, pp. 3405-9, 2006 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)", type "Antibodies"
Hosts Rabbit (185), Mouse (105), Goat (28), Sheep (16), Chicken (8)
Reactivities Human (239), Mouse (Murine) (147), Rat (Rattus) (146), Rabbit (35), Pig (Porcine) (31), Cow (Bovine) (21), Dog (Canine) (21), Chicken (13), Monkey (13), Cat (Feline) (8), Goat (8), Fish (7), Mammalian (7), Yeast (Saccharomyces cerevisiae) (6), Hamster (5), Bird (Avian) (4), Fruit Fly (Drosophila melanogaster) (4), Escherichia coli (E. coli) (3), Insect (3), Macaque (3), Xenopus laevis (3), Yeast (Saccharomyces Cerevisiae) (2), Amphibian (1), Bacteria (1), Chimpanzee (1), General (1), Saccharomyces cerevisiae (S. cerevisiae) (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (257), ELISA (151), Immunofluorescence (IF) (148), Immunocytochemistry (ICC) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Immunohistochemistry (IHC) (42), Immunoprecipitation (IP) (30), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Dot Blot (DB) (6), Immunodiffusion (ID) (6), Radioimmunoassay (RIA) (5), Immunoelectron Microscopy (IEM) (4), Dot Blot (Dot) (3), Flow Cytometry (FACS) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Fluorescence Microscopy (FM) (1), Immunostaining (ISt) (1), Purification (Purif) (1)
Conjugates Biotin (17), HRP (11), FITC (6), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), Gold (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy7 (4)
Epitopes C-Term (32), N-Term (16), Internal Region (8), AA 73-87 (2), AA 1-150 (1), AA 1-154 (1), AA 1-335 (1), AA 150-200 (1), AA 200-250 (1), AA 250-300 (1), AA 51-290 (1), C-Term, Arg248 (1), N-Term,AA 30-44 (1)