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Nanog Homeobox (NANOG) antibody

Details for Product No. ABIN258263, Supplier: Log in to see
Antigen
  • 2410002E02Rik
  • ecat4
  • ENK
Reactivity
Human, Pig (Porcine)
332
91
37
6
4
2
2
2
1
Host
Goat
220
103
41
2
Clonality
Polyclonal
Conjugate
Un-conjugated
16
16
15
15
12
12
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
274
201
109
70
59
52
38
37
24
14
8
4
4
3
2
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-QNQRMKSKRWQKNN corresponding to internal region according to NP_079141.2.
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Dog
Purification Immunogen affinity purified
Alternative Name Nanog (NANOG Antibody Abstract)
Background Gene Symbol: NANOG
Molecular Weight Theoretical MW: 35 kDa
Gene ID 79923, 71950, 414065
UniProt Q9H9S0
Research Area Embryogenesis, Cell Cycle, Embryonic stem cells
Application Notes Western Blot 0.03 - 0.1 μg/mL, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Peptide ELISA Detection limit 1:64000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 with 0.5 % BSA.
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Nanog Homeobox (NANOG) antibody (ABIN258263) (0.03ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary inc...
Immunofluorescence (IF) image for anti-Nanog Homeobox (NANOG) antibody (ABIN258263) (5ug/ml) staining (green) parts of a colony of induced pluriform stem cells derived f...
Product cited in: Vassena, Montserrat, Carrasco Canal, Aran, de Oñate, Veiga, Izpisua Belmonte: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Salisbury, Rodenberg, Sonnet, Hipp, Gannon, Vadakkan, Dickinson, Olmsted-Davis, Davis: "Sensory nerve induced inflammation contributes to heterotopic ossification." in: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2748-58, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Vackova, Novakova, Krylov, Okada, Kott, Fulka, Motlik: "Analysis of marker expression in porcine cell lines derived from blastocysts produced in vitro and in vivo." in: The Journal of reproduction and development, Vol. 57, Issue 5, pp. 594-603, 2011 (PubMed). (Sample species: Pig (Porcine)). Further details: Immunocytochemistry,Immunofluorescence

Monaco-Shawver, Schwartz, Tuluc, Guo, Lai, Gunnam, Kilpatrick, Banerjee, Douglas, Orange: "Substance P inhibits natural killer cell cytotoxicity through the neurokinin-1 receptor." in: Journal of leukocyte biology, Vol. 89, Issue 1, pp. 113-25, 2011 (PubMed).

Raya, Rodríguez-Pizà, Guenechea, Vassena, Navarro, Barrero, Consiglio, Castellà, Río, Sleep, González, Tiscornia, Garreta, Aasen, Veiga, Verma, Surrallés, Bueren, Izpisúa Belmonte: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, 2009 (PubMed).

Aasen, Raya, Barrero, Garreta, Consiglio, Gonzalez, Vassena, Bili?, Pekarik, Tiscornia, Edel, Boué, Izpisúa Belmonte: "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes." in: Nature biotechnology, Vol. 26, Issue 11, pp. 1276-84, 2008 (PubMed).

Hyslop, Stojkovic, Armstrong, Walter, Stojkovic, Przyborski, Herbert, Murdoch, Strachan, Lako: "Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages." in: Stem cells (Dayton, Ohio), Vol. 23, Issue 8, pp. 1035-43, 2005 (PubMed).