GFP antibody (Green Fluorescent Protein) (Biotin)

Details for Product anti-GFP Antibody No. ABIN258271, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
639
5
5
2
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2
1
1
Host
Mouse
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171
80
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16
9
8
2
Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is conjugated to Biotin
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27
14
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11
8
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5
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4
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3
2
2
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2
1
1
1
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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337
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96
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57
26
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2
1
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1
Options
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Immunogen The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Clone 9F9-F9
Isotype IgG2a
Specificity Reactivity is observed against wild type and recombinant forms of GFP. No reaction is seen against RFP.
Purification Protein A purified
Alternative Name GFP
Background Gene Symbol: GFP
Molecular Weight Theoretical MW: 27 kDa
Research Area Tags/Labels
Application Notes Western Blot 1:2000-1:10000, ELISA 1:10000-1:50000, Immunohistochemistry 1:1000-1:5000, Immunohistochemistry-Paraffin 1:10-1:500Use in In vitro assay reported in scientific literature (PMID 23125142) Use in dot blot reported in scientific literature (PMID 24497578) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein)  (Biotin) (ABIN258271) anti-Green Fluorescent Protein (GFP) antibody (Biotin)
Immunohistochemistry (IHC) image for anti-GFP antibody (Green Fluorescent Protein)  (Biotin) (ABIN258271) Immunohistochemistry: GFP Antibody (9F9.F9) [Biotin] [ABIN2582710] - Used 1:5000 As r...
Product cited in: Della Pia, Martinez: "Single domain antibodies as a powerful tool for high quality surface plasmon resonance studies." in: PLoS ONE, Vol. 10, Issue 3, pp. e0124303, 2015 (PubMed).

Sampson, Leyland, Dart: "Direct interaction between the actin-binding protein filamin-A and the inwardly rectifying potassium channel, Kir2.1." in: The Journal of biological chemistry, Vol. 278, Issue 43, pp. 41988-97, 2003 (PubMed).