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alpha Tubulin / TUBA4A / TUBA1 (N-Term) antibody

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Human, Mouse (Murine), Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide corresponding to residues near the N-term of human Alpha-Tubulin was used as immunogen.
Clone EP1332Y
Isotype IgG
Purification Tissue culture supernatant
Background Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis.It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulinbinds to alpha tubulin to form tubulin heterodimer which is post-translationally modified(1). The tubulin dimer complex binds to GTP and assembles onto the positive ends ofmicrotubules. After incorporation into the microtubules, bound GTP is hydrolyzed bybeta tubulin. The stability of the dimer in the microtubules is depended on presence ofbeta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubuleincorporation (2). For both type of tubulin, the carboxy-terminal tail is pos-translationallymodified to regulate associated protein binding at microtubule surface (3). A mutation onalpha tubulin has been linked to abnormal neuronal migration and neurodevelopmentaldisorders in human (4). Alternate Names: anti-Alpha tubulin ubiquitous antibody, anti-H2 ALPHA antibody, anti-TUBA1 antibody,anti-Tubulin alpha 1 chain antibody, anti-Tubulin alpha antibody, anti-Tubulin alpha ubiquitous chain antibody, anti-Tubulin K alpha 1 antibody.
Gene Symbol: TUBA1B
Gene ID 10376, 22143, 500929
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 50kDa.
Recommended dilutions: Flow Cytometry 1:10-100, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:250-500, Immunoprecipitation 1:10-100, Western Blot 1:1000-10000
Protocol Protocol specific for alpha Tubulin 4a Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
Western Blotting (WB) image for anti-alpha Tubulin / TUBA4A / TUBA1 (N-Term) antibody (ABIN258507) Western blot analysis on HeLa cell lysate using anti- ?-Tubulin RabMAb (cat. # 110-57...
Immunohistochemistry (IHC) image for anti-alpha Tubulin / TUBA4A / TUBA1 (N-Term) antibody (ABIN258507) Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-...
Immunofluorescence (IF) image for anti-alpha Tubulin / TUBA4A / TUBA1 (N-Term) antibody (ABIN258507) Imunofluorescent staining of HeLa cells using anti- ?-Tubulin RabMAb (cat# 110-57609)
Product cited in: Lee, Deng, Wang et al.: "MicroRNA-378 promotes cell survival, tumor growth, and angiogenesis by targeting SuFu and Fus-1 expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 51, pp. 20350-5, 2007 (PubMed).