Defensin, alpha 5, Paneth Cell-Specific (DEFA5) antibody

Antigen: Defensin, alpha 5, Paneth Cell-Specific (DEFA5)

Synonyms: DEF5, HD-5, MGC129728, DEFA5

Clonality: Monoclonal (8C8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN258712

Additional Information

Alternative name: Defensin alpha 5

Gene ID: 1670

Immunogen: Full-length proHD5 protein. [Swiss-Prot# Q01523] The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63(alanine).

Cross-Reactivity: Human

Isotype: IgG2b

Clone: 8C8

Description: Human neutrophil alpha-defensins (also designated HNPs) are small, cationic,cysteine-rich antimicrobial proteins that play important roles in innate immunity againstinfectious microbes such as bacteria, fungi and enveloped viruses. Alpha-defensins aresynthesized as inactive precursors and are activated by proteolytic cleavage by MMP-7.Paneth cells in small intestinal crypts secrete the alpha-defensins, which are alsotermed cryptidins in mice. Alpha-defensins 5 and 6 probably contribute to innatedefense of the GI mucosal surface by protecting against microbial invasion in states ofintestinal inflammation. Alternate Names: anti-HD5 antibody, anti-Defensin 5 antibody, anti-Defensin, alpha 5 antibody,anti-DEFA5 antibody, anti-DEF5 antibody. Related Diseases: Crohn',s Disease

Specificity: This antibody is specific for HD5. It does not cross-react with HD6, lysozyme or sPLA2. Species Reactivity: This antibody reacts with human HD5 protein. Other species have not been tested. Localization: Secreted protein.

Application Details

Application Notes: Suggested working dilutions: Immunohistochemistry-Paraffin 5-10 ug/ml

Concentration: 2.35 mg/ml

Purification: Purified

Buffer: Tris-glycine, 150mM NaCl. Preservative: 0.05% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

Human skeletal muscle myocytes showing moderate nuclear and faint cytoplasmic staining using ABIN258712.

Publications

Wehkamp, Salzman, Porter et al.: "Reduced Paneth cell alpha-defensins in ileal Crohn's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 50, pp. 18129-34, 2005 (PubMed).