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Defensin, alpha 5, Paneth Cell-Specific (DEFA5) (cleaved) antibody

Details for Product No. ABIN258713, Supplier: Log in to see
Antigen
  • DEFA5
  • Defcr24
  • Defcr4
  • Rd5
  • defensin 6
  • defensin 7
  • defensin-5
  • DEF5
  • HD-5
  • Defa
  • DEFA1
  • Defa29
  • Defcr29
  • Defcr5
Epitope
cleaved
9
7
4
3
1
1
1
1
1
Reactivity
Human
39
3
3
2
Host
Mouse
21
20
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
2
2
1
1
1
1
Application
Dot Blot (DB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
28
23
15
7
6
6
3
2
2
1
Supplier
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Immunogen Full-length human Defensin alpha 5. The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine).
Clone 8C8
Isotype IgG2b
Specificity This is specific for HD5. It does not cross-react with HD6, lysozyme or sPLA2.
Purification Protein G purified
Alternative Name Defensin alpha 5 (DEFA5 Antibody Abstract)
Background Human neutrophil alpha-defensins (also designated HNPs) are small, cationic,cysteine-rich antimicrobial proteins that play important roles in innate immunity againstinfectious microbes such as bacteria, fungi and enveloped viruses. Alpha-defensins aresynthesized as inactive precursors and are activated by proteolytic cleavage by MMP-7.Paneth cells in small intestinal crypts secrete the alpha-defensins, which are alsotermed cryptidins in mice. Alpha-defensins 5 and 6 probably contribute to innatedefense of the GI mucosal surface by protecting against microbial invasion in states ofintestinal inflammation. Alternate Names: anti-HD5 antibody, anti-Defensin 5 antibody, anti-Defensin, alpha 5 antibody,anti-DEFA5 antibody, anti-DEF5 antibody. Related Diseases: Crohn',s Disease
Gene Symbol: DEFA5
Gene ID 1670
UniProt Q01523
Pathways
Application Notes This Defensin alpha 5 antibody is useful for Immunohistochemistry paraffin embedded sections, Western blot and Immunocytochemistry/Immunofluorescence. In Western blot a band is observed ~10 kDa. Dot Blot and ELISA were reported in scientific literature.
Recommended dilutions: Dot Blot, ELISA, Flow Cytometry 1 µg per million cells, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunohistochemistry 5-10 µg/mL, Immunohistochemistry-Paraffin 5-10 µg/mL, Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2.35 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Defensin, alpha 5, Paneth Cell-Specific (DEFA5) (cleaved) antibody (ABIN258713) Human skeletal muscle myocytes showing moderate nuclear and faint cytoplasmic stainin...
Product cited in: Wehkamp, Salzman, Porter et al.: "Reduced Paneth cell alpha-defensins in ileal Crohn's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 50, pp. 18129-34, 2005 (PubMed).