Defensin, alpha 5, Paneth Cell-Specific (DEFA5) antibody

Details for Product No. ABIN258713, Supplier: Log in to see
Antigen
  • DEFA5
  • Defcr24
  • Defcr4
  • Rd5
  • defensin 6
  • defensin 7
  • defensin-5
  • DEF5
  • HD-5
  • Defa
  • DEFA1
  • Defa29
  • Defcr29
  • Defcr5
  • defensin, alpha 5, Paneth cell-specific
  • alpha-defensin 1A
  • defensin alpha 24
  • defensin, alpha, 5
  • DEFA5
  • MNP1A
  • Defa24
  • Defa5
Reactivity
Human
55
2
2
2
Host
Mouse
36
21
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
47
37
31
25
22
18
17
5
2
1
1
1
1
Options
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Immunogen Full-length human Defensin alpha 5 [Swiss-Prot# Q01523]. The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine).
Clone 8C8
Isotype IgG2b
Specificity This is specific for HD5. It does not cross-react with HD6, lysozyme or sPLA2.
Purification Protein G purified
Alternative Name Defensin alpha 5 (DEFA5 Antibody Abstract)
Background Gene Symbol: DEFA5
Molecular Weight Theoretical MW: 10 kDa
Gene ID 1670
UniProt Q01523
Application Notes Western Blot 1:1000, Flow Cytometry 1 μg per million cells, ELISA, Immunohistochemistry 5-10 μg/mL, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunohistochemistry-Paraffin 5-10 μg/mL, Dot BlotThis Defensin alpha 5 antibody is useful for Immunohistochemistry paraffin embedded sections, Western blot and Immunocytochemistry/Immunofluorescence. In Western blot a band is observed ~10 kDa. Dot Blot and ELISA were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 2.35 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Defensin, alpha 5, Paneth Cell-Specific (DEFA5) antibody (ABIN258713) Human skeletal muscle myocytes showing moderate nuclear and faint cytoplasmic stainin...
Flow Cytometry (FACS) image for anti-Defensin, alpha 5, Paneth Cell-Specific (DEFA5) antibody (ABIN258713) Flow Cytometry: Defensin alpha 5 Antibody (8C8) [ABIN2587122] - Intracellular flow cy...
Product cited in: Liu, Naito, Liu, VanDussen, Haritunians, Li, Endo, Kawai, Nagasaki, Kinouchi, McGovern, Shimosegawa, Kakuta, Stappenbeck: "LRRK2 but not ATG16L1 is associated with Paneth cell defect in Japanese Crohn's disease patients." in: JCI insight, Vol. 2, Issue 6, pp. e91917, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Jung, Kim, Yeom, Yoo, Shin, Yoo, Kang, Lee: "Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer." in: Journal of gastroenterology, Vol. 47, Issue 1, pp. 37-48, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Kim, Kang, Lee, Jung, Lee, Yu, Kim, Lee: "Profiling of differentially expressed proteins in stage IV colorectal cancers with good and poor outcomes." in: Journal of proteomics, Vol. 75, Issue 10, pp. 2983-97, 2012 (PubMed).

Bukurova, Nikitina, Khankin, Krasnov, Lisitsin, Karpov, Beresten: "[Identification of protein markers for serum diagnosis of cancer based on microRNA expression profiling]." in: Molekuliarnaia biologiia, Vol. 45, Issue 2, pp. 376-81, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Wehkamp, Chu, Shen, Feathers, Kays, Lee, Bevins: "Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues." in: FEBS letters, Vol. 580, Issue 22, pp. 5344-50, 2006 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Wehkamp, Salzman, Porter, Nuding, Weichenthal, Petras, Shen, Schaeffeler, Schwab, Linzmeier, Feathers, Chu, Lima, Fellermann, Ganz, Stange, Bevins: "Reduced Paneth cell alpha-defensins in ileal Crohn's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 50, pp. 18129-34, 2005 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry

Shen, Porter, Reynoso, Shen, Ghosh, Connor, Drazba, Rho, Gramlich, Li, Ormsby, Sy, Ganz, Bevins: "Human defensin 5 expression in intestinal metaplasia of the upper gastrointestinal tract." in: Journal of clinical pathology, Vol. 58, Issue 7, pp. 687-94, 2005 (PubMed). (Sample species: Human). Further details: Dot Blot,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),ELISA,Immunohistochemistry