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APOE antibody (Apolipoprotein E)

Details for Product anti-APOE Antibody No. ABIN258786, Supplier: Log in to see
Antigen
  • AD2
  • ad2
  • AI255918
  • Apo-E
  • apoe
  • APOEA
  • apoprotein
  • im:7036787
  • LDLCQ5
  • LPG
  • wu:fb69a05
  • zgc:110064
Reactivity
Human, Mouse (Murine)
297
80
39
7
5
2
1
Host
Mouse
149
108
92
Clonality (Clone)
Monoclonal ()
Conjugate
This APOE antibody is un-conjugated
25
19
13
5
5
5
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
249
206
92
58
24
16
15
14
14
9
8
5
5
4
4
3
2
2
2
2
2
2
2
1
1
1
Supplier
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Immunogen Purified human ApoE [UniProt# P02649]
Clone WUE-4
Isotype IgG1 kappa
No Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) It does not appear to react with rat,brain tissue.
Purification Protein G purified
Alternative Name Apolipoprotein E/ApoE (APOE Antibody Abstract)
Background Gene Symbol: APOE
Molecular Weight Theoretical MW: 36 kDa
Gene ID 348
UniProt P02649
Application Notes Western Blot 2 μg/mL, Flow Cytometry 1 μg per million cells, ELISA 1:100-1:2000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for ApoE Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS, 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the mouse anti-ApoE primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer'sinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-APOE antibody (Apolipoprotein E) (ABIN258786) anti-Apolipoprotein E (APOE) antibody
Flow Cytometry (FACS) image for anti-APOE antibody (Apolipoprotein E) (ABIN258786) Flow Cytometry: ApoE Antibody (WUE-4) [ABIN2587851] - Intracellular flow cytometric s...
Western Blotting (WB) image for anti-APOE antibody (Apolipoprotein E) (ABIN258786) Western Blot: ApoE Antibody (WUE-4) [ABIN2587851] - 50 ug protein per lane for liver ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-APOE antibody (Apolipoprotein E) (ABIN258786) Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [ABIN2587851] -...
Immunofluorescence (IF) image for anti-APOE antibody (Apolipoprotein E) (ABIN258786) Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [ABIN2...
Product cited in: Tambini, Pera, Kanter, Yang, Guardia-Laguarta, Holtzman, Sulzer, Area-Gomez, Schon: "ApoE4 upregulates the activity of mitochondria-associated ER membranes." in: EMBO reports, Vol. 17, Issue 1, pp. 27-36, 2016 (PubMed). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Jung, Kim, Park, Luo, Chang: "Roles of human apolipoprotein E in the infectivity and replication of hepatitis C virus genotype 2a." in: Journal of microbiology (Seoul, Korea), Vol. 54, Issue 6, pp. 451-8, 2016 (PubMed). (Sample species: Human).

Pinçon, Coulombe, Chouinard-Watkins, Plourde: "Human apolipoprotein E allele and docosahexaenoic acid intake modulate peripheral cholesterol homeostasis in mice." in: The Journal of nutritional biochemistry, Vol. 34, pp. 83-8, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Chouinard-Watkins, Pinçon, Coulombe, Spencer, Massenavette, Plourde: "A Diet Rich in Docosahexaenoic Acid Restores Liver Arachidonic Acid and Docosahexaenoic Acid Concentrations in Mice Homozygous for the Human Apolipoprotein E ε4 Allele." in: The Journal of nutrition, Vol. 146, Issue 7, pp. 1315-21, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Fukuhara, Wada, Nakamura, Ono, Shiokawa, Yamamoto, Motomura, Okamoto, Okuzaki, Yamamoto, Saito, Wakita, Koike, Matsuura: "Amphipathic ?-helices in apolipoproteins are crucial to the formation of infectious hepatitis C virus particles." in: PLoS pathogens, Vol. 10, Issue 12, pp. e1004534, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting,ELISA

Lee, Tse, Smith, Landreth: "Apolipoprotein E promotes β-amyloid trafficking and degradation by modulating microglial cholesterol levels." in: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 2032-44, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: ELISA

Hirsch-Reinshagen, Donkin, Stukas, Chan, Wilkinson, Fan, Parks, Kuivenhoven, Lütjohann, Pritchard, Wellington: "LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins." in: Journal of lipid research, Vol. 50, Issue 5, pp. 885-93, 2009 (PubMed). (Sample species: Mouse (Murine)). Further details: ELISA

Wahrle, Jiang, Parsadanian, Hartman, Bales, Paul, Holtzman: "Deletion of Abca1 increases Abeta deposition in the PDAPP transgenic mouse model of Alzheimer disease." in: The Journal of biological chemistry, Vol. 280, Issue 52, pp. 43236-42, 2005 (PubMed).

Fryer, Demattos, McCormick, ODell, Spinner, Bales, Paul, Sullivan, Parsadanian, Bu, Holtzman: "The low density lipoprotein receptor regulates the level of central nervous system human and murine apolipoprotein E but does not modify amyloid plaque pathology in PDAPP mice." in: The Journal of biological chemistry, Vol. 280, Issue 27, pp. 25754-9, 2005 (PubMed). (Sample species: Human). Further details: ELISA

Krul, Tikkanen, Schonfeld: "Heterogeneity of apolipoprotein E epitope expression on human lipoproteins: importance for apolipoprotein E function." in: Journal of lipid research, Vol. 29, Issue 10, pp. 1309-25, 1989 (PubMed). (Sample species: Human). Further details: Western Blotting