Apolipoprotein E (APOE) antibody

Details for Product No. ABIN258786
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Antigen
Synonyms ad2, apoprotein, im:7036787, wu:fb69a05, zgc:110064, apoe, AI255918, AD2, LDLCQ5, LPG, APOEA, Apo-E
Reactivity
Human
(181), (44), (31), (5), (5), (1), (1), (1)
Host
Mouse
(109), (68), (46)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(13), (8), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(157), (127), (50), (42), (20), (19), (16), (10), (9), (8), (8), (8), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 0.025 mL
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Catalog No. ABIN258786
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Immunogen Purified human ApoE
Clone WUE-4
Isotype IgG1 kappa
No Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) It does not appear to react with rat,brain tissue.
Purification Protein G purified
Alternative Name Apolipoprotein E (Apo E)
Background Lipoproteins are responsible for carrying cholesterol and other fats through thebloodstream as little packages and are essential for the normal breakdown of thesemolecules. In particular, apolipoprotein E is a major component of specific lipoproteinscalled very low-density lipoproteins (VLDL). A major function of VLDLs is to removeexcess cholesterol from the blood and carry it to the liver for processing. Maintainingnormal levels of cholesterol is essential for the prevention of cardiovascular diseases,including heart attacks and strokes.Apolipoprotein E is a type of lipoprotein. Apolipoprotein E is associated with severalcardiovascular disorders. There are three isoforms (slightly different forms) of the ApoElipoprotein. These three isoforms are known as ApoE2, E3 and E4. ApoE2 seems to beone of several genetic factors that plays a part in increased risk of heart attacks andstrokes and ApoE4 is a risk factor for coronary artery disease. As well, ApoE4 has beenbeen suggested to play a role in type 2 Alzheimer disease. Alternate Names: anti-Apolipoprotein E antibody, anti-Apo-E antibody. Related Diseases: Alzheimer',s Disease, Atherosclerosis, Coronary Artery Disease
Gene Symbol: APOE
Gene ID 348
UniProt P02649
Research Area Metabolism
Application Notes This ApoE antibody is useful for Western blot, ELISA, Immunohistochemistry and Immunoprecipitation. In Western blot a band is observed at ~36 kDa, representing the ApoE protein.
Recommended dilutions: ELISA 1:100-1:2000, Flow Cytometry 1 µg per million cells, Immunohistochemistry 1:50-1:200, Immunoprecipitation 1:10-1:500, Western Blot 2 µg/mL
Protocol Protocol specific for ApoE Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS, 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the mouse anti-ApoE primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer'sinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.85 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-Apolipoprotein E (APOE) antibody anti-Apolipoprotein E (APOE) antibody
General Krul, Tikkanen, Schonfeld: "Heterogeneity of apolipoprotein E epitope expression on human lipoproteins: importance for apolipoprotein E function." in: Journal of lipid research, Vol. 29, Issue 10, pp. 1309-25, 1989 (PubMed).

Fryer, Demattos, McCormick et al.: "The low density lipoprotein receptor regulates the level of central nervous system human and murine apolipoprotein E but does not modify amyloid plaque pathology in PDAPP mice." in: The Journal of biological chemistry, Vol. 280, Issue 27, pp. 25754-9, 2005 (PubMed).

Wahrle, Jiang, Parsadanian et al.: "Deletion of Abca1 increases Abeta deposition in the PDAPP transgenic mouse model of Alzheimer disease." in: The Journal of biological chemistry, Vol. 280, Issue 52, pp. 43236-42, 2005 (PubMed).

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