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Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 400-500), (Internal Region) antibody

Details for Product No. ABIN258876, Supplier: Log in to see
Antigen
  • 4.1B
  • DAL-1
  • Dal1
  • DAL1
  • DAL1P
  • EPB4.1L3
  • Epb4.1l3
  • EPB41L1
  • Epb41l3
  • Kiaa0987
  • NBL3
Epitope
Internal Region, AA 400-500
15
15
14
10
5
3
2
2
1
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus), Xenopus laevis
55
21
20
17
11
3
1
1
1
1
Host
Rabbit
24
16
15
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
2
2
2
1
1
1
1
1
Application
Simple Western (SimWes), Western Blotting (WB)
51
24
3
3
2
2
1
Supplier
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Immunogen A synthetic peptide made to an internal region of human EPB41L3 (within residues 400-500). [Swiss-Prot# Q9Y2J2]
Purification Immunogen affinity purified
Alternative Name EPB41L3 (EPB41L3 Antibody Abstract)
Background Gene Symbol: EPB41L3
Gene ID 23136, 13823, 116724
UniProt Q9Y2J2
Research Area Cancer, Cytoskeleton, Microfilaments
Application Notes Western Blot 2 μg/mL, Simple Western 1:50
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for EPB41L3 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 38 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-Band 4.1 like-protein 3 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1.15 mg/mL
Buffer PBS, 30 % glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 400-500), (Internal Region) antibody (ABIN258876) Detection of Band 4.1 in HeLa whole cell lysate using ABIN258876.
Western Blotting (WB) image for anti-Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 400-500), (Internal Region) antibody (ABIN258876) Detection of Band 4.1 in mouse brain lysate using ABIN258876.
Simple Western (SimWes) image for anti-Erythrocyte Membrane Protein Band 4.1 Like 3 (EPB41L3) (AA 400-500), (Internal Region) antibody (ABIN258876) Simple Western: EPB41L3 Antibody [ABIN2588767] - Simple Western lane view shows a spe...
Product cited in: Wong, Haack, Kissil, Barry, Bronson, Shen, Whittaker, Crowley, Hynes: "Protein 4.1B suppresses prostate cancer progression and metastasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 31, pp. 12784-9, 2007 (PubMed). (Sample species: Human). Further details: Western Blotting

McCarty, Cook, Hynes: "An interaction between {alpha}v{beta}8 integrin and Band 4.1B via a highly conserved region of the Band 4.1 C-terminal domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 38, pp. 13479-83, 2005 (PubMed). (Sample species: Primate). Further details: Western Blotting