SLC9A3 antibody (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3) (C-Term)

Details for Product anti-SLC9A3 Antibody No. ABIN258888, Supplier: Log in to see
Antigen
  • SLC9A3
  • 9030624O13Rik
  • AI930210
  • NHE-3
  • NHE3
  • Nhe3
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3
  • sodium/hydrogen exchanger 3 (nhe3)
  • Sodium/hydrogen exchanger 3
  • Na/H exchanger 3
  • solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3
  • SLC9A3
  • AaeL_AAEL001503
  • GL50803_102647
  • Smp_045680
  • NHE-3
  • Slc9a3
Alternatives
anti-Rabbit SLC9A3 antibody for Immunohistochemistry (Frozen Sections)
Epitope
C-Term
31
20
19
2
2
2
2
1
1
1
1
Reactivity
Rabbit, Rat (Rattus)
136
84
62
39
38
36
4
1
1
1
1
Host
Mouse
79
72
1
Clonality (Clone)
Monoclonal ()
Conjugate
This SLC9A3 antibody is un-conjugated
10
8
7
6
6
6
5
5
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
144
111
79
51
37
25
21
16
2
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Fusion protein representing the C-terminal 131 amino acids of rabbit NHE3. [UniProt# P26432]
Clone 19F5
Isotype IgG1
Specificity This antibody is specific for NHE3. It does not show any cross-reactivity with any other NHE isoforms.
No Cross-Reactivity Human
Purification Protein G purified
Alternative Name NHE3/SLC9A3 (SLC9A3 Antibody Abstract)
Background Gene Symbol: SLC9A3
Molecular Weight Theoretical MW: 93 kDa
Gene ID 6550
UniProt P26432
Pathways Proton Transport
Application Notes Western Blot 0.5 μg/mL, ELISA, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500This NHE3 antibody is useful for Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections and Western blot, where a band can be seen at ~93 kDa. Immunocytochemistry/Immunofluorescence and ELISA were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Sodium / Hydrogen Exchanger 3 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the mouse anti-NHE3 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3) (C-Term) antibody (ABIN258888) Detection of NHE3 in rat kidney lysate using ABIN258888.
Product cited in: Saad, Zhang, Yong, Yaghobian, Wong, Kelly, Chen, Pollock: "Role of the EGF receptor in PPARγ-mediated sodium and water transport in human proximal tubule cells." in: Diabetologia, Vol. 56, Issue 5, pp. 1174-82, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Kocinsky, Girardi, Biemesderfer, Nguyen, Mentone, Orlowski, Aronson: "Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites." in: American journal of physiology. Renal physiology, Vol. 289, Issue 2, pp. F249-58, 2005 (PubMed).

Sabolić, Herak-Kramberger, Ljubojević, Biemesderfer, Brown: "NHE3 and NHERF are targeted to the basolateral membrane in proximal tubules of colchicine-treated rats." in: Kidney international, Vol. 61, Issue 4, pp. 1351-64, 2002 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Bagnis, Marsolais, Biemesderfer, Laprade, Breton: "Na+/H+-exchange activity and immunolocalization of NHE3 in rat epididymis." in: American journal of physiology. Renal physiology, Vol. 280, Issue 3, pp. F426-36, 2001 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Girardi, Titan, Malnic, Rebouças: "Chronic effect of parathyroid hormone on NHE3 expression in rat renal proximal tubules." in: Kidney international, Vol. 58, Issue 4, pp. 1623-31, 2000 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunohistochemistry

Biemesderfer, Nagy, DeGray, Aronson: "Specific association of megalin and the Na+/H+ exchanger isoform NHE3 in the proximal tubule." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17518-24, 1999 (PubMed). (Sample species: Rabbit). Further details: Immunoprecipitation

Zhang, Norian, Magyar, Holstein-Rathlou, Mircheff, McDonough: "In vivo PTH provokes apical NHE3 and NaPi2 redistribution and Na-K-ATPase inhibition." in: The American journal of physiology, Vol. 276, Issue 5 Pt 2, pp. F711-9, 1999 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Biemesderfer, DeGray, Aronson: "Membrane topology of NHE3. Epitopes within the carboxyl-terminal hydrophilic domain are exoplasmic." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12391-6, 1998 (PubMed). (Sample species: Rabbit). Further details: Western Blotting,Immunoprecipitation,ELISA

Biemesderfer, Rutherford, Nagy, Pizzonia, Abu-Alfa, Aronson: "Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney." in: The American journal of physiology, Vol. 273, Issue 2 Pt 2, pp. F289-99, 1997 (PubMed). Further details: Immunocytochemistry,Western Blotting,ELISA,Immunofluorescence,Immunohistochemistry (Frozen Sections)

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