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Actin, beta (ACTB) antibody
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB), ELISA
|15 references available|
|Quantity||0.1 ml (2.0 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Alternative name||Actin beta / ACTB|
|Gene ID||60, 11461, 81822|
|Immunogen||Synthetic peptide conjugated to KLH, corresponding to amino acids 1-14 of Xenopuslaevis Actin (beta).|
|Cross-Reactivity||Cow (Bovine), Dog (Canine), Chicken, Cat (Feline), Fish, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)|
|Description||This is an ideal reagent for use as a cell extract loading control on Western blot. It hasbeen used as such in most of the references below. Molecular weight of beta actin is42kD. Alternate Names: Anti-A X actin like protein antibody, Anti-Actx antibody, Anti-Beta cytoskeletal actinantibody, Anti-Melanoma X actin antibody, Anti-PS1TP5BP1 antibody, Anti-Beta-actinantibody, Anti-Beta actin antibody, Anti-Actin, beta antibody.|
|Specificity||Species Reactivity: Cross-reacts with Cat, Chicken, Cow, Dog, Drosophila melanogaster, Fish (carp andgoldfish), Guinea Pig, Human, Leech, Mouse, Pig, Rabbit, Rat, and Sheep. Detectsbeta actin in a wide variety of tissues and species. Does not cross react with adultcardiac or skeletal muscle or amoeba beta actin. Not yet tested in other species.|
|Synonyms||PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1|
|Application Notes||Western Blot 0.5 - 1 ug/ml,Immunocytochemistry 10 - 40 ug/ml,Acetone Fixation. Positive Controls: Cultured human or chicken fibroblasts, as described in Liao et al.|
|Purification||Aff - Purified|
|Buffer||0.01 M PBS, pH 7.4. Preservative: 15mm sodium azide|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Restrictions||For Research Use only|
|anti-Actin, beta (ACTB) antibody|
North, Gimona, Cross et al.: "Calponin is localised in both the contractile apparatus and the cytoskeleton of smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 437-44, 1994 (PubMed).
North, Gimona, Lando et al.: "Actin isoform compartments in chicken gizzard smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 445-55, 1994 (PubMed).
Gimona, Vandekerckhove, Goethals et al.: "Beta-actin specific monoclonal antibody." in: Cell motility and the cytoskeleton, Vol. 27, Issue 2, pp. 108-16, 1994 (PubMed).
Liao, Xu, Wargovich: "Direct reprobing with anti-beta-actin antibody as an internal control for western blotting analysis." in: BioTechniques, Vol. 28, Issue 2, pp. 216-8, 2000 (PubMed).
Adler, Huang, Wolin et al.: "Oxidant stress leads to impaired regulation of renal cortical oxygen consumption by nitric oxide in the aging kidney." in: Journal of the American Society of Nephrology : JASN, Vol. 15, Issue 1, pp. 52-60, 2003 (PubMed).
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Watters, Kraja, Meucci et al.: "Genome-wide discovery of loci influencing chemotherapy cytotoxicity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 32, pp. 11809-14, 2004 (PubMed).
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Chong, Costain, Marriott et al.: "Differential display polymerase chain reaction reveals increased expression of striatal rat glia-derived nexin following chronic clozapine treatment." in: The pharmacogenomics journal, Vol. 4, Issue 6, pp. 379-87, 2004 (PubMed).
Adler, Huang: "Oxidant stress in kidneys of spontaneously hypertensive rats involves both oxidase overexpression and loss of extracellular superoxide dismutase." in: American journal of physiology. Renal physiology, Vol. 287, Issue 5, pp. F907-13, 2004 (PubMed).
Sung, Yang, Uryu et al.: "Modulation of nuclear factor-kappa B activity by indomethacin influences A beta levels but not A beta precursor protein metabolism in a model of Alzheimer's disease." in: The American journal of pathology, Vol. 165, Issue 6, pp. 2197-206, 2004 (PubMed).
Abramovich, Parborell, Tesone: "Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats." in: Biology of reproduction, Vol. 75, Issue 3, pp. 434-41, 2006 (PubMed).