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Details for Product No. ABIN258983

Actin, beta (ACTB) antibody

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ACTA1, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Bap47, CG4027, Dmel\\CG4027, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin, beta-acti ... show more
»Alternatives Cow (Bovine), Dog (Canine), Chicken, Cat (Feline), Fish, Guinea Pig, Human, Leech, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
»Alternatives Un-conjugated
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 15 references available
Catalog no. ABIN258983
Quantity 0.1 mL
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Immunogen Synthetic peptide conjugated to KLH, corresponding to amino acids 1-14 of Xenopuslaevis Actin (beta).
Clone AC-15
Isotype IgG1
Specificity Species Reactivity: Cross-reacts with Cat, Chicken, Cow, Dog, Drosophila melanogaster, Fish (carp andgoldfish), Guinea Pig, Human, Leech, Mouse, Pig, Rabbit, Rat, and Sheep. Detectsbeta actin in a wide variety of tissues and species. Does not cross react with adultcardiac or skeletal muscle or amoeba beta actin. Not yet tested in other species.
Cross-Reactivity Cow (Bovine), Dog (Canine), Chicken, Cat (Feline), Fish, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
Purification Affinity Purified
Alternative Name Actin beta / ACTB
Background This is an ideal reagent for use as a cell extract loading control on Western blot. It hasbeen used as such in most of the references below. Molecular weight of beta actin is42kD. Alternate Names: Anti-A X actin like protein antibody, Anti-Actx antibody, Anti-Beta cytoskeletal actinantibody, Anti-Melanoma X actin antibody, Anti-PS1TP5BP1 antibody, Anti-Beta-actinantibody, Anti-Beta actin antibody, Anti-Actin, beta antibody.
Gene ID 60, 11461, 81822
Application Notes Western Blot 0.5 - 1 ug/ml,Immunocytochemistry 10 - 40 ug/ml,Acetone Fixation. Positive Controls: Cultured human or chicken fibroblasts, as described in Liao et al.
Restrictions For Research Use only
Format Liquid
Concentration 2.0 mg/ml
Buffer 0.01 M PBS, pH 7.4. Preservative: 15mm sodium azide
Preservative Sodium azide
Storage 4 °C
North, Gimona, Cross et al.: "Calponin is localised in both the contractile apparatus and the cytoskeleton of smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 437-44, 1994 (PubMed).

North, Gimona, Lando et al.: "Actin isoform compartments in chicken gizzard smooth muscle cells." in: Journal of cell science, Vol. 107 ( Pt 3), pp. 445-55, 1994 (PubMed).

Gimona, Vandekerckhove, Goethals et al.: "Beta-actin specific monoclonal antibody." in: Cell motility and the cytoskeleton, Vol. 27, Issue 2, pp. 108-16, 1994 (PubMed).

Liao, Xu, Wargovich: "Direct reprobing with anti-beta-actin antibody as an internal control for western blotting analysis." in: BioTechniques, Vol. 28, Issue 2, pp. 216-8, 2000 (PubMed).

Adler, Huang, Wolin et al.: "Oxidant stress leads to impaired regulation of renal cortical oxygen consumption by nitric oxide in the aging kidney." in: Journal of the American Society of Nephrology : JASN, Vol. 15, Issue 1, pp. 52-60, 2003 (PubMed).

Thornton, Kudo, Rayman et al.: "Degradation of NF-kappa B in T cells by gangliosides expressed on renal cell carcinomas." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 6, pp. 3480-90, 2004 (PubMed).

Hawkins, Fischer: "Inhibition of insulin synthesis by cyproheptadine: effects on translation." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 79, Issue 2, pp. 258-65, 2004 (PubMed).

Dietz, Souan, Knutson et al.: "Imatinib mesylate inhibits T-cell proliferation in vitro and delayed-type hypersensitivity in vivo." in: Blood, Vol. 104, Issue 4, pp. 1094-9, 2004 (PubMed).

Watters, Kraja, Meucci et al.: "Genome-wide discovery of loci influencing chemotherapy cytotoxicity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 32, pp. 11809-14, 2004 (PubMed).

Laughlin, McGugan, Powell et al.: "Involvement of raft-like plasma membrane domains of Entamoeba histolytica in pinocytosis and adhesion." in: Infection and immunity, Vol. 72, Issue 9, pp. 5349-57, 2004 (PubMed).

Mukherjee, Abate, Seyfried: "Antiangiogenic and proapoptotic effects of dietary restriction on experimental mouse and human brain tumors." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 16, pp. 5622-9, 2004 (PubMed).

Chong, Costain, Marriott et al.: "Differential display polymerase chain reaction reveals increased expression of striatal rat glia-derived nexin following chronic clozapine treatment." in: The pharmacogenomics journal, Vol. 4, Issue 6, pp. 379-87, 2004 (PubMed).

Adler, Huang: "Oxidant stress in kidneys of spontaneously hypertensive rats involves both oxidase overexpression and loss of extracellular superoxide dismutase." in: American journal of physiology. Renal physiology, Vol. 287, Issue 5, pp. F907-13, 2004 (PubMed).

Sung, Yang, Uryu et al.: "Modulation of nuclear factor-kappa B activity by indomethacin influences A beta levels but not A beta precursor protein metabolism in a model of Alzheimer's disease." in: The American journal of pathology, Vol. 165, Issue 6, pp. 2197-206, 2004 (PubMed).

Abramovich, Parborell, Tesone: "Effect of a vascular endothelial growth factor (VEGF) inhibitory treatment on the folliculogenesis and ovarian apoptosis in gonadotropin-treated prepubertal rats." in: Biology of reproduction, Vol. 75, Issue 3, pp. 434-41, 2006 (PubMed).

Alternatives for antigen "Actin, beta (ACTB)", type "Antibodies"
Hosts (207), (125), (21), (2), (2)
Reactivities (323), (194), (176), (72), (58), (57), (44), (30), (29), (25), (23), (19), (16), (12), (11), (6), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (314), (134), (98), (68), (52), (51), (33), (20), (18), (16), (10), (4), (4), (3), (2), (2), (1), (1)
Conjugates (14), (11), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (95), (23), (12), (9), (6), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1)