Tenascin C (TNC) (Domain B) antibody

Details for Product No. ABIN259023, Supplier: Log in to see
Antigen
  • tn
  • 150-225
  • GMEM
  • GP
  • HXB
  • JI
  • TN
  • TN-C
  • AI528729
  • C130033P17Rik
  • Hxb
  • Ten
  • cytotactin
  • tenascin-C
  • P230
  • tenc
  • wu:fk04d02
  • tenascin C
  • tnc
  • TNC
  • MCYG_00667
  • Tnc
Alternatives
anti-Human Tenascin C antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
Domain B
18
8
7
6
5
5
5
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3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
269
82
71
5
3
2
1
Host
Mouse
148
119
13
4
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
20
18
14
5
5
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3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
155
120
102
66
60
42
38
38
27
26
7
5
5
5
1
Options
Supplier
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Supplier Product No.
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Immunogen Recombinant human Tenascin C [Swiss-Prot# P24821].
Clone 4C8MS
Isotype IgG1
Specificity This antibody specifically reacts with Domain B on FNIII repeats of Tenascin C.
Characteristics Tenascin C is an extracellular matrix protein that is expressed in various tissues during development, disease or injury. Tenascin C inhibits HIV infection in immune cells by binding to a chemokine coreceptor site on the HIV-1 envelope protein, thereby blocking the virus' entry into the host cells. Tenascin C expression in the stroma of certain tumors is often associated with a poor prognosis.
Purification Protein A purified
Alternative Name Tenascin C (TNC Antibody Abstract)
Background Gene Symbol: TNC
Gene ID 3371
UniProt P24821
Research Area Stem Cells, Extracellular Matrix
Pathways Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
Application Notes Western Blot 5 μg/mL, Flow Cytometry 1 μg per million cells, ELISA, Immunohistochemistry 1:25 - 1:50, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:25 - 1:50, Immunohistochemistry-Frozen, CyTOF-readySee PMID: 21871891 for the ICC/IF use of thsi product. For its use in IHC-P, it is recommended to incubate primary antibody for atleast 2 hours at room temperature followed by ON incubation at 4C.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-Paraffin Embedded Sections Protocol Specific for NB110-68136: Tenascin C Antibody (4C8MS)Immunohistochemistry-Paraffin Embedded Sections for NB110-68136Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Tenascin C (TNC) (Domain B) antibody (ABIN259023) anti-Tenascin C (TNC) (Domain B) antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tenascin C (TNC) (Domain B) antibody (ABIN259023) Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [ABIN2590236] - IHC analys...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tenascin C (TNC) (Domain B) antibody (ABIN259023) Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [ABIN2590236] - IHC analys...
Flow Cytometry (FACS) image for anti-Tenascin C (TNC) (Domain B) antibody (ABIN259023) Flow Cytometry: Tenascin C Antibody (4C8MS) [ABIN2590236] - Intracellular flow cytome...
 image for anti-Tenascin C (TNC) (Domain B) antibody (ABIN259023) Flow (Intracellular): Tenascin C Antibody (4C8MS) .
Product cited in: Bhattacharyya, Wang, Morales-Nebreda, Feng, Wu, Zhou, Lafyatis, Lee, Hinchcliff, Feghali-Bostwick, Lakota, Budinger, Raparia, Tamaki, Varga: "Tenascin-C drives persistence of organ fibrosis." in: Nature communications, Vol. 7, pp. 11703, 2016 (PubMed). Further details: Western Blotting,Immunoprecipitation

Janune, Abd El Kader, Aoyama, Nishida, Tabata, Kubota, Takigawa: "Novel role of CCN3 that maintains the differentiated phenotype of articular cartilage." in: Journal of bone and mineral metabolism, 2016 (PubMed).

de Sousa, Gurgel, Ramos, Trindade, de Faro Valverde, Carneiro, Cangussú, Pinheiro, Dos Santos: "Infrared LED light therapy influences the expression of fibronectin and tenascin in skin wounds of malnourished rats--a preliminary study." in: Acta histochemica, Vol. 116, Issue 7, pp. 1185-91, 2014 (PubMed). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Ago, Kemmochi, Morita, Yafune, Shiraki, Mitsumori, Shibutani: "Direct progression of capsular invasive carcinomas from subcapsular focal hyperplasias induced by hypothyroidism-mediated tumor promotion in a rat two-stage thyroid carcinogenesis model." in: Journal of cancer research and clinical oncology, Vol. 139, Issue 3, pp. 395-401, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

James, Ross, Nicola, Steele, Ambalavanan: "VARA attenuates hyperoxia-induced impaired alveolar development and lung function in newborn mice." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 304, Issue 11, pp. L803-12, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Kemmochi, Shimamoto, Shiraki, Onda, Hasumi, Suzuki, Mitsumori, Shibutani: "Promoting effects of carminic acid-enriched cochineal extracts on capsular invasive thyroid carcinomas through targeting activation of angiogenesis in rats." in: The Journal of toxicological sciences, Vol. 37, Issue 3, pp. 475-82, 2012 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Kemmochi, Yamamichi, Shimamoto, Onda, Hasumi, Suzuki, Mitsumori, Shibutani: "Lac color inhibits development of rat thyroid carcinomas through targeting activation of plasma hyaluronan-binding protein." in: Experimental biology and medicine (Maywood, N.J.), Vol. 237, Issue 6, pp. 728-38, 2012 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Yoshimura, Michishita, Ohkusu-Tsukada, Takahashi: "Increased presence of stromal myofibroblasts and tenascin-C with malignant progression in canine mammary tumors." in: Veterinary pathology, Vol. 48, Issue 1, pp. 313-21, 2011 (PubMed).

Balasenthil, Chen, Lott, Chen, Carter, Grizzle, Frazier, Sen, Killary: "A migration signature and plasma biomarker panel for pancreatic adenocarcinoma." in: Cancer prevention research (Philadelphia, Pa.), Vol. 4, Issue 1, pp. 137-49, 2011 (PubMed). (Sample species: Human). Further details: ELISA

Yamamoto, Okui, Tatebe, Shinohara, Hirata: "Regeneration of the perineurium after microsurgical resection examined with immunolabeling for tenascin-C and alpha smooth muscle actin." in: Journal of anatomy, Vol. 218, Issue 4, pp. 413-25, 2011 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Janune, Kubota, Nishida, Kawaki, Perbal, Iida, Takigawa: "Novel effects of CCN3 that may direct the differentiation of chondrocytes." in: FEBS letters, Vol. 585, Issue 19, pp. 3033-40, 2011 (PubMed).

Guttery, Shaw, Lloyd, Pringle, Walker: "Expression of tenascin-C and its isoforms in the breast." in: Cancer metastasis reviews, Vol. 29, Issue 4, pp. 595-606, 2010 (PubMed).

Richter, Tost, Franz, Altendorf-Hofmann, Junker, Borsi, Neri, Kosmehl, Wunderlich, Berndt: "B and C domain containing tenascin-C: urinary markers for invasiveness of urothelial carcinoma of the urinary bladder?" in: Journal of cancer research and clinical oncology, Vol. 135, Issue 10, pp. 1351-8, 2009 (PubMed). (Sample species: Human). Further details: ELISA,Immunohistochemistry (Frozen Sections),Immunohistochemistry

El-Karef, Kaito, Tanaka, Ikeda, Nishioka, Fujita, Inada, Adachi, Kawada, Nakajima, Imanaka-Yoshida, Yoshida: "Expression of large tenascin-C splice variants by hepatic stellate cells/myofibroblasts in chronic hepatitis C." in: Journal of hepatology, Vol. 46, Issue 4, pp. 664-73, 2007 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Kääriäinen, Nummela, Soikkeli, Yin, Lukk, Jahkola, Virolainen, Ora, Ukkonen, Saksela, Hölttä: "Switch to an invasive growth phase in melanoma is associated with tenascin-C, fibronectin, and procollagen-I forming specific channel structures for invasion." in: The Journal of pathology, Vol. 210, Issue 2, pp. 181-91, 2006 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Tamaoki, Imanaka-Yoshida, Yokoyama, Nishioka, Inada, Hiroe, Sakakura, Yoshida: "Tenascin-C regulates recruitment of myofibroblasts during tissue repair after myocardial injury." in: The American journal of pathology, Vol. 167, Issue 1, pp. 71-80, 2005 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Tsunoda, Inada, Kalembeyi, Imanaka-Yoshida, Sakakibara, Okada, Katsuta, Sakakura, Majima, Yoshida: "Involvement of large tenascin-C splice variants in breast cancer progression." in: The American journal of pathology, Vol. 162, Issue 6, pp. 1857-67, 2003 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

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