Nucleus Accumbens Associated 1, BEN and BTB (POZ) Domain Containing (NACC1) antibody

Details for Product No. ABIN259549, Supplier: Log in to see
Antigen
  • BEND8
  • BTBD14B
  • BTBD30
  • NAC-1
  • NAC1
  • NACC1
  • 2010001H03Rik
  • 4930511N13Rik
  • Btbd14b
  • Nac1
  • Nac-1
  • EIEE6
  • FEB3
  • FEB3A
  • FHM3
  • GEFSP2
  • HBSCI
  • Nav1.1
  • SCN1
  • SMEI
  • nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
  • sodium channel, voltage-gated, type I, alpha subunit
  • NACC1
  • Nacc1
  • SCN1A
Reactivity
Human, Mouse (Murine), Rat (Rattus)
87
44
40
8
6
6
5
4
2
1
1
1
Host
Mouse
40
35
16
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
68
33
31
28
24
21
14
13
8
1
1
Options
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Immunogen Recombinant human Nac1 protein. [UniProt# Q96RE7]
Clone 3
Purification Protein G purified
Alternative Name NAC1 (NACC1 Antibody Abstract)
Background Gene Symbol: NACC1
Molecular Weight Theoretical MW: 57 kDa
Gene ID 112939
UniProt Q96RE7
Application Notes Western Blot 0.3 μg/mL, Flow Cytometry 1 μg per million cells, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunoprecipitation Assay dependent, Immunohistochemistry-Paraffin 1:100This Nac1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin,Immunoprecipitation and Flow Cytometry. In WB a band is seen at ~57 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Nac1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 20 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-NAC1 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Nucleus Accumbens Associated 1, BEN and BTB (POZ) Domain Containing (NACC1) antibody (ABIN259549) Detection of NAC-1 in HeLa nuclear extracts using ABIN259549.
Immunohistochemistry (IHC) image for anti-Nucleus Accumbens Associated 1, BEN and BTB (POZ) Domain Containing (NACC1) antibody (ABIN259549) Nuclear staining of paraffin-embedded ovarian cancer cells using ABIN259549.
Flow Cytometry (FACS) image for anti-Nucleus Accumbens Associated 1, BEN and BTB (POZ) Domain Containing (NACC1) antibody (ABIN259549) Flow Cytometry: Nac1 Antibody (3) [ABIN2595495] - Intracellular flow cytometric stain...
Immunofluorescence (IF) image for anti-Nucleus Accumbens Associated 1, BEN and BTB (POZ) Domain Containing (NACC1) antibody (ABIN259549) Immunocytochemistry/Immunofluorescence: Nac1 Antibody (3) [ABIN2595495] - Staining in...
Product cited in: Zhang, Ren, Guo, Ji, Hu, Zhang, Xu, Zhu, Ming, Yuan, Ren, Song, Yang: "Nucleus accumbens-associated protein-1 promotes glycolysis and survival of hypoxic tumor cells via the HDAC4-HIF-1α axis." in: Oncogene, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Nakayama, Rahman, Rahman, Nakamura, Sato, Katagiri, Ishibashi, Ishikawa, Iida, Razia, Ishikawa, Kyo: "Nucleus accumbens-1/GADD45GIP1 axis mediates cisplatin resistance through cellular senescence in ovarian cancer." in: Oncology letters, Vol. 13, Issue 6, pp. 4713-4719, 2017 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Yap, Sysa-Shah, Bolon, Wu, Gao, Herlinger, Wang, Faiola, Huso, Gabrielson, Wang, Wang, Shih: "Loss of NAC1 expression is associated with defective bony patterning in the murine vertebral axis." in: PLoS ONE, Vol. 8, Issue 7, pp. e69099, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Korutla, Furlong, Mackler: "NAC1, A POZ/BTB protein interacts with Parkin and may contribute to Parkinson's disease." in: Neuroscience, Vol. 257, pp. 86-95, 2013 (PubMed). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Rahman, Nakayama, Rahman, Katagiri, Katagiri, Ishibashi, Ishikawa, Iida, Nakayama, Otsuki, Nakayama, Miyazaki: "Fatty acid synthase expression associated with NAC1 is a potential therapeutic target in ovarian clear cell carcinomas." in: British journal of cancer, Vol. 107, Issue 2, pp. 300-7, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Rahman, Nakayama, Ishikawa, Rahman, Katagiri, Katagiri, Ishibashi, Iida, Yamada, Miyazaki: "NAC1, a BTB/POZ protein overexpressed in uterine sarcomas." in: Anticancer research, Vol. 32, Issue 9, pp. 3841-5, 2012 (PubMed).

Scofield, Korutla, Jackson, Kalivas, Mackler: "Nucleus Accumbens 1, a Pox virus and Zinc finger/Bric-a-brac Tramtrack Broad protein binds to TAR DNA-binding protein 43 and has a potential role in Amyotrophic Lateral Sclerosis." in: Neuroscience, Vol. 227, pp. 44-54, 2012 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Shih, Nakayama, Wu, Nakayama, Zhang, Wang: "Amplification of the ch19p13.2 NACC1 locus in ovarian high-grade serous carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 24, Issue 5, pp. 638-45, 2011 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Wu, Hung, Ren, Shih, Tseng: "Cell cycle-dependent alteration in NAC1 nuclear body dynamics and morphology." in: Physical biology, Vol. 8, Issue 1, pp. 15005, 2011 (PubMed).

Constantin, McCullough, Mahajan, Greenhaff: "Novel events in the molecular regulation of muscle mass in critically ill patients." in: The Journal of physiology, Vol. 589, Issue Pt 15, pp. 3883-95, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Nakayama, Nakayama, Davidson, Sheu, Jinawath, Santillan, Salani, Bristow, Morin, Kurman, Wang, Shih: "A BTB/POZ protein, NAC-1, is related to tumor recurrence and is essential for tumor growth and survival." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 49, pp. 18739-44, 2006 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence