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LMO2 antibody (LIM Domain Only 2 (Rhombotin-Like 1))

Details for Product anti-LMO2 Antibody No. ABIN259553, Supplier: Log in to see
Antigen
  • Rbtn-2
  • Rbtn2
  • Rhom-2
  • Ttg2
  • RBTN2
  • RBTNL1
  • RHOM2
  • TTG2
  • wu:fc83a08
  • zgc:111930
  • LMO-2
  • lmo2-A
  • xlmo2
  • rhombotin-2
  • Rhombotin-2
  • LIM domain only 2 (rhombotin-like 1)
  • LIM domain only 2
  • rbtn2
  • LOC100232598
  • Lmo2
  • LMO2
  • lmo2
Alternatives
anti-Human LMO2 antibody for Chromatin Immunoprecipitation
Reactivity
Human
133
24
23
21
4
4
4
4
4
3
3
2
2
2
1
Host
Mouse
75
55
3
Clonality (Clone)
Monoclonal ()
Conjugate
This LMO2 antibody is un-conjugated
5
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
115
47
47
37
31
18
9
8
6
2
1
Options
Supplier
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Immunogen Recombinant human LMO2. [UniProt# P25791]
Clone 1A9-3B11
Isotype IgG1 kappa
Purification Protein G purified
Alternative Name LMO2 (LMO2 Antibody Abstract)
Background Gene Symbol: LMO2
Molecular Weight Theoretical MW: 24 kDa
Gene ID 4005
UniProt P25791
Research Area Cardiovascular
Pathways Chromatin Binding
Application Notes Western Blot 0.5 μg/mL, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:500This LMO2 antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Chromatin Immunoprecipitation and Immunohistochemistry on paraffin-embedded sections. In WB a band can be seen at ~24 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for LMO2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-LMO2 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-LMO2 antibody (LIM Domain Only 2 (Rhombotin-Like 1)) (ABIN259553) Detection of LMO2 in Ramos cell lysate using ABIN259552.
Immunofluorescence (IF) image for anti-LMO2 antibody (LIM Domain Only 2 (Rhombotin-Like 1)) (ABIN259553) Immunofluorescent labeling of LMO2 (green) in tonsil tissue using ABIN259552.
Product cited in: Natkunam, Zhao, Mason, Chen, Taidi, Jones, Hammer, Hamilton Dutoit, Lossos, Levy: "The oncoprotein LMO2 is expressed in normal germinal-center B cells and in human B-cell lymphomas." in: Blood, Vol. 109, Issue 4, pp. 1636-42, 2007 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)