HSD3B1 antibody (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1)

Details for Product anti-HSD3B1 Antibody No. ABIN259557, Supplier: Log in to see
Antigen
  • HSD3B
  • HSD3B1
  • MGC131206
  • 3BETAHSD
  • HSDB3
  • HSDB3A
  • I
  • SDR11E1
  • 3B-HSD
  • D3Ertd383e
  • 3b-HSD
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
  • HSD3B1
  • hsd3b1
  • Hsd3b1
  • HSD3B2
Alternatives
anti-Rat (Rattus) HSD3B1 antibody for ELISA
Reactivity
Baboon, Cow (Bovine), Human, Primate, Rat (Rattus)
102
37
24
19
18
18
2
2
1
1
Host
Mouse
51
37
15
Clonality (Clone)
Monoclonal ()
Conjugate
This HSD3B1 antibody is un-conjugated
6
6
6
5
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
77
40
35
24
23
23
18
11
3
Options
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Immunogen JEG Choriocarcinoma cells
Clone FDO66Q
Isotype IgG1
Purification Protein G purified
Alternative Name HSD3B1 (HSD3B1 Antibody Abstract)
Background Gene Symbol: HSD3B1
Gene ID 3283
Research Area Hormones, Cancer
Pathways Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis
Application Notes Western Blot, Immunohistochemistry 10-20 μg/mL, Immunocytochemistry/Immunofluorescence 10-20 μg/mL, Immunohistochemistry-Paraffin 10-20 μg/mL, Immunohistochemistry-Frozen 10-20 μg/mLThis HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for HSD3B1 Antibody Immunohistochemistry-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds betweenchanges.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-HSD3B1 antibody (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1) (ABIN259557) Staining of placental villi using ABIN259556.
Product cited in: Palaniappan, Menon: "Luteinizing hormone/human chorionic gonadotropin-mediated activation of mTORC1 signaling is required for androgen synthesis by theca-interstitial cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 26, Issue 10, pp. 1732-42, 2012 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Mao, Kurman, Jeng, Huang, Shih: "HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions." in: The American journal of surgical pathology, Vol. 32, Issue 2, pp. 236-42, 2008 (PubMed).

Irving-Rodgers, Krupa, Rodgers et al.: "Cholesterol side-chain cleavage cytochrome P450 and 3beta-hydroxysteroid dehydrogenase expression and the concentrations of steroid hormones in the follicular fluids of different phenotypes of ..." in: Biology of reproduction, Vol. 69, Issue 6, pp. 2022-8, 2003 (PubMed).

Irving-Rodgers, Bathgate, Ivell, Domagalski, Rodgers et al.: "Dynamic changes in the expression of relaxin-like factor (INSL3), cholesterol side-chain cleavage cytochrome p450, and 3beta-hydroxysteroid dehydrogenase in bovine ovarian follicles during growth and ..." in: Biology of reproduction, Vol. 66, Issue 4, pp. 934-43, 2002 (PubMed). (Sample species: Cow (Bovine)). Further details: Immunohistochemistry (Frozen Sections)

Mueller, Hawes, Wright, Petropoulos, DeBoni, Firgaira, Morley, Turner, Jones: "Isolation of fetal trophoblast cells from peripheral blood of pregnant women." in: Lancet, Vol. 336, Issue 8709, pp. 197-200, 1990 (PubMed). Further details: Immunocytochemistry,Immunofluorescence