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Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1) antibody

Details for Product No. ABIN259557, Supplier: Log in to see
Antigen
  • 11-DH
  • 11-beta-HSD1
  • CORTRD2
  • HDL
  • HSD11
  • HSD11B
  • HSD11L
  • SDR26C1
  • hsd11
  • hsd11b
  • LRRGT00065
Reactivity
Human, Monkey, Baboon
90
41
30
4
4
4
3
1
1
1
Host
Mouse
72
28
7
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
89
52
19
18
12
10
4
4
2
1
1
1
1
1
Supplier
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Immunogen JEG Choriocarcinoma cells
Clone FDO66Q
Isotype IgG1
Purification Protein G purified
Alternative Name 3-beta-HSD1 / HSD3B1 (HSD11B1 Antibody Abstract)
Background 3-beta hydroxysteroid dehydrogenase (HSD3B1) is a bifunctional enzyme involved inthe oxidative conversion of ketosteroids that plays an important role in the synthesis ofall steroid hormones. There are two HSD3B1 proteins, designated type I and type II, thatare expressed by different genes and function in different areas of the body. HSD3B1has also been shown to be a highly specific and sensitive trophoblast-associated marker(Mao et. al). Alternate Names: anti-3-beta-HSD I antibody, anti-Trophoblast antigen FDO161G antibody, anti-HSD3B1antibody, anti-3BH antibody, anti-HSDB3A antibody, anti-3BHSD antibody.
Gene Symbol: HSD3B1
Gene ID 3283
Pathways
Application Notes This HSD3B1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections and frozen sections. There has been negative feedback with the use of this antibody in Western blot.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 10-20 µg/mL, Immunohistochemistry 10-20 µg/mL, Immunohistochemistry-Frozen 10-20 µg/mL, Immunohistochemistry-Paraffin 10-20 µg/mL, Western Blot
Protocol Protocol specific for HSD3B1 Antibody Immunohistochemistry-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds betweenchanges.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Immunohistochemistry (IHC) image for anti-Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1) antibody (ABIN259557) Staining of placental villi using ABIN259556.
Product cited in: Mao, Kurman, Jeng et al.: "HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions." in: The American journal of surgical pathology, Vol. 32, Issue 2, pp. 236-42, 2008 (PubMed).

Background publications Mueller, Hawes, Wright et al.: "Isolation of fetal trophoblast cells from peripheral blood of pregnant women." in: Lancet, Vol. 336, Issue 8709, pp. 197-200, 1990 (PubMed).